Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.193 extracted from

  • Li, L.; Shin, S.Y.; Lee, S.J.; Moon, J.S.; Im, W.T.; Han, N.S.
    Production of ginsenoside F2 by using Lactococcus lactis with enhanced expression of beta-glucosidase gene from Paenibacillus mucilaginosus (2016), J. Agric. Food Chem., 64, 2506-2512 .
    View publication on PubMed
Search for more literature for 3.2.1.193

Application

Application Comment Organism
synthesis production of the pharmacologically active minor ginsenoside F2 from the major ginsenosides Rb1 and Rd by using the recombinant Lactococcus lactis strain expressing heterologous the beta-glucosidase gene Paenibacillus mucilaginosus

Cloned(Commentary)

Cloned (Comment) Organism
gene BglPm, recombinant expression of C-terminally His6-tagged enzyme in Lactococcus lactis strain NZ9000, method optimization Paenibacillus mucilaginosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rb1 + H2O Paenibacillus mucilaginosus
-
 ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb1 + H2O Paenibacillus mucilaginosus KCTC 3870
-
 ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rd + H2O Paenibacillus mucilaginosus
-
 ginsenoside F2 + D-glucose
-
 ?
ginsenoside Rd + H2O Paenibacillus mucilaginosus KCTC 3870
-
 ginsenoside F2 + D-glucose
-
 ?
ginsenoside Rg3 + H2O Paenibacillus mucilaginosus
-
 ginsenoside Rh2 + D-glucose
-
 ?
ginsenoside Rg3 + H2O Paenibacillus mucilaginosus KCTC 3870
-
 ginsenoside Rh2 + D-glucose
-
 ?
additional information Paenibacillus mucilaginosus bioconversion of major ginsenosides to F2 during milk fermentation ?
-
 ?
additional information Paenibacillus mucilaginosus KCTC 3870 bioconversion of major ginsenosides to F2 during milk fermentation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus mucilaginosus
-
-
-
Paenibacillus mucilaginosus KCTC 3870
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Lactococcus lactis strain NZ9000 by nickel affinity chromatography Paenibacillus mucilaginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rb1 + H2O
-
 Paenibacillus mucilaginosus ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb1 + H2O
-
 Paenibacillus mucilaginosus KCTC 3870 ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rd + H2O
-
 Paenibacillus mucilaginosus ginsenoside F2 + D-glucose
-
 ?
ginsenoside Rd + H2O
-
 Paenibacillus mucilaginosus KCTC 3870 ginsenoside F2 + D-glucose
-
 ?
ginsenoside Rg3 + H2O
-
 Paenibacillus mucilaginosus ginsenoside Rh2 + D-glucose
-
 ?
ginsenoside Rg3 + H2O
-
 Paenibacillus mucilaginosus KCTC 3870 ginsenoside Rh2 + D-glucose
-
 ?
additional information bioconversion of major ginsenosides to F2 during milk fermentation Paenibacillus mucilaginosus ?
-
 ?
additional information recombinant His-tagged enzyme BGL hydrolyzes the outer glucose moiety of C-3 and C-20 positions of the major ginsenosides (Rb1 and Rd) and produces the minor ginsenoside F2: the pathway is Rb1 -> Rd -> F2. Furthermore, the enzyme transforms the minor ginsenoside Rg3 to Rh2: pathway Rg3 -> Rh2 Paenibacillus mucilaginosus ?
-
 ?
additional information bioconversion of major ginsenosides to F2 during milk fermentation Paenibacillus mucilaginosus KCTC 3870 ?
-
 ?
additional information recombinant His-tagged enzyme BGL hydrolyzes the outer glucose moiety of C-3 and C-20 positions of the major ginsenosides (Rb1 and Rd) and produces the minor ginsenoside F2: the pathway is Rb1 -> Rd -> F2. Furthermore, the enzyme transforms the minor ginsenoside Rg3 to Rh2: pathway Rg3 -> Rh2 Paenibacillus mucilaginosus KCTC 3870 ?
-
 ?

Synonyms

Synonyms Comment Organism
Bgl
-
 Paenibacillus mucilaginosus
BglPm
-
 Paenibacillus mucilaginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paenibacillus mucilaginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Paenibacillus mucilaginosus