Protein Variants | Comment | Organism |
---|---|---|
additional information | constructions of chimeric lysozymes are carried out by swapping the N-terminal and C-terminal domains between phage T7 and K11 lysozymes. This technique generates two chimeras, T7K11-lysozyme (N-terminal T7 domain and C-terminal K11 domain) and K11T7-lysozyme (N-terminal K11 domain and C-terminal T7 domain), which are both enzymatically active. The amidase activity of T7K11-lysozyme is comparable with the parental enzymes while K11T7-lysozyme exhibits an activity that is approximately 45% greater than the wild-type lysozymes. The chimeric constructs have optimum pH of 7.2-7.4 similar to the parental lysozymes but exhibit greater thermal stabilities. The chimeras inhibit transcription comparable with the parental lysozymes depending on the source of their N-terminals. Domain swapping technique localizes the N-terminal region as the domain responsible for the transcription inhibition specificity of the wild type T7 and K11 lysozymes | Escherichia phage T7 |
additional information | constructions of chimeric lysozymes are carried out by swapping the N-terminal and C-terminal domains between phage T7 and K11 lysozymes. This technique generates two chimeras, T7K11-lysozyme (N-terminal T7 domain and C-terminal K11 domain) and K11T7-lysozyme (N-terminal K11 domain and C-terminal T7 domain), which are both enzymatically active. The amidase activity of T7K11-lysozyme is comparable with the parental enzymes while K11T7-lysozyme exhibits an activity that is approximately 45% greater than the wild-type lysozymes. The chimeric constructs have optimum pH of 7.2-7.4 similar to the parental lysozymes but exhibit greater thermal stabilities. The chimeras inhibit transcription comparable with the parental lysozymes depending on the source of their N-terminals. Domain swapping technique localizes the N-terminal region as the domain responsible for the transcription inhibition specificity of the wild type T7 and K11 lysozymes | Enterobacteria phage K11 |
Organism | UniProt | Comment | Textmining |
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Enterobacteria phage K11 | - |
- |
- |
Escherichia phage T7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
rapid purification scheme in purifying both the wild-type and chimeric lysozymes | Escherichia phage T7 |
rapid purification scheme in purifying both the wild-type and chimeric lysozymes | Enterobacteria phage K11 |