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(GlcNAc)6 + H2O
(GlcNAc)3
-
-
and smaller amounts of (GlcNAc)2 and (GlcNAc)4 endo-splitting, hydrolyzes preferentially the third glycosidic linkage from the nonreducing end
-
?
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O
4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
cell wall of Bacillus subtilis + H2O
?
-
-
-
-
?
cell wall of Escherichia coli + H2O
?
cell wall of Micrococcus luteus + H2O
?
cell wall of Micrococcus lysodeikticus + H2O
?
cell wall of Streptococcus agalactiae + H2O
?
-
-
-
-
?
cell wall of Vibrio alginolyticus + H2O
?
-
-
-
-
?
chito-oligosaccharide + H2O
?
oligosaccharides with a degree of polymerization between three and six units, product analysis by mass and NMR spectrometry
-
-
?
chitohexaose + H2O
?
-
-
-
?
chitohexaose + H2O
chitobiose + chitotetraose
chitopentaose + H2O
N-acetyl-D-glucosamine + chitobiose + chitotetraose + chitotriose
-
amount in descending order, binding kinetics with chitotriose, overview
-
?
chitopentaose + H2O
N-acetyl-D-glucosamine + chitobiose + chitotriose + chitotetraose
-
amount in descending order, binding kinetics with chitotriose, overview
-
?
chitosan + H2O
N-acetyl-D-glucosamine + ?
DA48
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
colloidal chitin + H2O
?
-
-
-
-
?
ethylene glycol chitin + H2O
?
ethylene glycol chitin + H2O
sugars
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + (GlcNAc)2
-
-
main products
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + ?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta
-
-
-
?
glycol chitin + H2O
chitin oligosaccharides
L-gamma-glutamine-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
substrate for isopeptidase activity of isoform cLys3
-
-
?
lyophilized cell wall of Micrococcus luteus + H2O
?
lyophilized cell walls of Micrococcus lysodiekticus + H2O
?
-
-
-
-
?
Micrococcus lysodeikticus cell wall + H2O
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
?
eight amino acid residues interact with the N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose oligomer: Arg73, Gly102, Asn103, Leu56, Ala107, Val109, Ala110, and Lys33
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose
-
wild-type protein hydrolyzes N,N',N'',N''',N''''-pentaacetylchitopentaose almost completely on 140 min reaction. N,N',N'',N'''-tetraacetylchitotetraose is is hydrolyzed mainly to N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose with much less cleavage into GlcNAc + N,N',N'',N'''-tetraacetylchitotetraose
-
-
?
NodRm-IV + H2O
NodRm-II + NodRm-III
-
-
-
?
NodRm-IV(Ac,S) + H2O
NodRm-II + NodRm-III
-
-
-
?
NodRm-IV(S) + H2O
NodRm-II + NodRm-III
-
-
-
?
NodRm-V(S) + H2O
NodRm-II + NodRm-III
-
-
-
?
p-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + H2O
?
-
-
-
-
?
p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
?
peptidoglycan + H2O
N-acetylaminosaccharides
additional information
?
-
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O
4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose
-
a synthetic fluorogenic substrate
-
-
?
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O
4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose
-
a synthetic fluorogenic substrate
-
-
?
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O
4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose
-
a synthetic fluorogenic substrate
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
-
?
cell wall of Escherichia coli + H2O
?
-
-
-
-
?
cell wall of Escherichia coli + H2O
?
-
-
-
-
?
cell wall of Micrococcus luteus + H2O
?
-
-
-
?
cell wall of Micrococcus luteus + H2O
?
-
-
-
?
cell wall of Micrococcus luteus + H2O
?
-
substrate for muramidase activity of isoform cLys3
-
-
?
cell wall of Micrococcus luteus + H2O
?
-
-
-
?
cell wall of Micrococcus luteus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
cell wall of Micrococcus lysodeikticus + H2O
?
-
-
-
-
?
chitin + H2O
sugars
-
-
-
-
?
chitin + H2O
sugars
-
-
reducing
?
chitohexaose + H2O
chitobiose + chitotetraose
-
-
mass spectrometry analysis
-
?
chitohexaose + H2O
chitobiose + chitotetraose
-
-
mass spectrometry analysis
-
?
chitohexaose + H2O
chitobiose + chitotetraose
-
-
mass spectrometry analysis
-
?
chitopentaose + H2O
?
-
-
-
?
chitopentaose + H2O
?
-
-
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
-
-
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
-
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
-
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
-
-
-
?
chitotetraose + H2O
chitotriose + N-acetylglucosamine
-
-
-
?
colloidal chitin
sugars
-
-
-
-
?
colloidal chitin
sugars
-
-
-
-
?
3.2.1.17 lysozyme
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