Application | Comment | Organism |
---|---|---|
additional information | the organophosphorus-hydrolyzing enzymes such as Brevundimonas diminuta phosphotriesterase (PTE) are used for development of alternative catalytic bioscavengers to treat organophosphorus agents, e.g. pesticides and warfare agents, intoxications of humans | Brevundimonas diminuta |
Cloned (Comment) | Organism |
---|---|
gene opd, recombinant expression of wild-type and mutant MBP-tagged enzymes in Escherichia coli strain BL21(DE3) | Brevundimonas diminuta |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a PTE mutant dubbed C23 is engineered, exhibiting reversed stereoselectivity and high catalytic efficiency (kcat/KM) for the hydrolysis of the toxic enantiomers of nerve agents VX, CVX, and VR. The other mutants A53, IV-A1, IV-H3, B141, and RD1-G83 are less effective, activities and mutant-substrate docking, overview. The only exception is IV-A1 with amiton. Most variants are highly efficient when hydrolyzing N,N-diisopropyl substrates and were 2fold less efficient with N,N-di-ethyl substrates and 5fold less efficient with N,N-di-methyl substrates | Brevundimonas diminuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics of wild-type and mutant enzymes, overview | Brevundimonas diminuta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | P0A434 | i.e. Pseudomonas diminuta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-propan-2-yl methylphosphonofluoridate + H2O | i.e. sarin | Brevundimonas diminuta | isopropyl phosphate methylphosphonate + fluoride | - |
? | |
2-methoxycarbonyl-1-methylvinyl dimethyl phosphate + H2O | i.e. mevinphos | Brevundimonas diminuta | ? | - |
? | |
3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O | i.e. soman | Brevundimonas diminuta | Pinacolyl methylphosphonate + fluoride | - |
? | |
cyclohexylmethylphosphonofluoridate + H2O | i.e. cyclosarin | Brevundimonas diminuta | cyclohexyl methylphosphonate + fluoride | - |
? | |
diethyl (3,5,6-trichloropyridin-2-yl) phosphate + H2O | i.e. chlorpyrifos-oxon | Brevundimonas diminuta | ? | - |
? | |
diethyl 2-(dimethoxyphosphorylsulfanyl)butanedioate + H2O | i.e. malaoxon | Brevundimonas diminuta | ? | - |
? | |
ethyl dimethylphosphoramidocyanidate + H2O | i.e. tabun | Brevundimonas diminuta | ? | - |
? | |
additional information | the enzyme also exhibits activity of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview | Brevundimonas diminuta | ? | - |
? | |
O,O-diethyl-S-[2-diethylaminoethyl]thiophosphate + H2O | i.e. amiton or VG | Brevundimonas diminuta | ? | - |
? | |
O,O-diethyl-S-[2-diisopropylaminoethyl]thiophosphate + H2O | i.e. diisopropyl-amiton | Brevundimonas diminuta | ? | - |
? | |
O,O-diethyl-S-[2-dimethylaminoethyl]thiophosphate + H2O | i.e. dimethyl-amiton | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-di-n-propylaminoethyl)methylphosphonothiolate + H2O | i.e. n-propyl-VX | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-diethylaminoethyl)ethylphosphonothiolate + H2O | i.e. VE | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O | i.e. VM | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-diisopropylaminoethyl)ethylphosphonothiolate + H2O | i.e. isopropyl-VE | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | i.e. VX | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-dimethylaminoethyl)ethylphosphonothiolate + H2O | i.e. methyl-VE | Brevundimonas diminuta | ? | - |
? | |
O-ethyl-S-(2-dimethylaminoethyl)methylphosphonothiolate + H2O | i.e. dimethyl-VX | Brevundimonas diminuta | ? | - |
? | |
O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O | i.e. VR or Russian VX | Brevundimonas diminuta | ? | - |
? | |
O-methyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O | i.e. methyl-VM | Brevundimonas diminuta | ? | - |
? | |
O-n-butyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O | i.e. CVX or Chinese VX | Brevundimonas diminuta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.8.2 | Brevundimonas diminuta |
OP-hydrolyzing enzyme | - |
Brevundimonas diminuta |
opd | - |
Brevundimonas diminuta |
organophosphorus-hydrolyzing enzyme | - |
Brevundimonas diminuta |
phosphotriesterase | - |
Brevundimonas diminuta |
PTE | - |
Brevundimonas diminuta |
PTE S5 | - |
Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brevundimonas diminuta |