Protein Variants | Comment | Organism |
---|---|---|
A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S | mutant constructed fo expression of soluble and active enzyme | Flavobacterium sp. |
L271/Y309A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, 2fold increase in reactivity with substrate demeton-S-methyl | Flavobacterium sp. |
L271A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, similar soluble expression | Flavobacterium sp. |
L271A/Y309A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, exhibits a 150fold higher catalytic efficiency for V-type nerve agents such as S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate than the wild-type | Flavobacterium sp. |
T172A/L271A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, decrease in activity | Flavobacterium sp. |
T172A/L271A/Y309A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, decrease in activity | Flavobacterium sp. |
T172A/Y309A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, 2fold increase in reactivity with substrate demeton-S-methyl | Flavobacterium sp. |
Y309A | mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, increase in soluble expression | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication | Flavobacterium sp. | |
5.87 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | mutant L271A/Y309A, pH 8.5, temperature not specified in the publication | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
demeton-S-methyl + H2O | - |
Flavobacterium sp. | 2-ethylsulfanyl-ethanethiol + dimethyl phosphate | - |
? | |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | i.e. nerve agent VX | Flavobacterium sp. | O-ethyl hydrogen methylphosphonothioate + 2-[di(propan-2-yl)amino]ethanol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication | Flavobacterium sp. | |
0.15 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | mutant L271A/Y309A, pH 8.5, temperature not specified in the publication | Flavobacterium sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00486 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication | Flavobacterium sp. | |
0.0249 | - |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate | mutant L271A/Y309A, pH 8.5, temperature not specified in the publication | Flavobacterium sp. |