Protein Variants | Comment | Organism |
---|---|---|
D229N/N120D | site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant | Loligo vulgaris |
D229N/N175D | site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant | Loligo vulgaris |
E21Q/N120D | site-directed mutagenesis, inactive mutant | Loligo vulgaris |
E21Q/N175D | site-directed mutagenesis, inactive mutant | Loligo vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O,O-dicyclopentylphosphoroamidate | substrate analogue, competitive inhibition, binding structure, direct coordination of the phosphoryl oxygen by the active site catalytic calcium ion, overview | Loligo vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for catalysis, bound at the active site, coordinating residues are E21 and D229 | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | catalytic mechanism | Loligo vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarin + H2O | a highly toxic organophosphorus nerve agent | Loligo vulgaris | methylphosphonofluoride acid + ? | - |
? | |
soman + H2O | i.e. pinacolyl methylphosphonofluoridate, a highly toxic organophosphorus nerve agent | Loligo vulgaris | methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol | - |
? | |
tabun + H2O | a highly toxic organophosphorus nerve agent | Loligo vulgaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFPase | - |
Loligo vulgaris |
diisopropyl fluorophosphatase | - |
Loligo vulgaris |
phosphotriesterase | - |
Loligo vulgaris |