Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cefazolin | inhibits the serum and liver PON1, competitive inhibition | Homo sapiens | |
gentamycin sulfate | inhibits the serum and liver PON1, noncompetitive inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.16 | - |
paraoxon | pH 8.0, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lipid particle | the enzyme is associated to HDL | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 227fold by ammonium sulfate fractionation and L-tyrosine-1-naphthylamine hydrophobic interaction chromatography to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
hepatoma cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
serum | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1730 | - |
purified serum enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
paraoxon + H2O | - |
Homo sapiens | diethylphosphate + 4-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
paraoxonase | - |
Homo sapiens |
PON1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in vivo and in vitro inhibition kinetic analysis, overview | Homo sapiens | |
0.0012 | - |
cefazolin | pH 8.0, 37°C, serum PON1, versus paraoxon | Homo sapiens | |
0.026 | - |
gentamycin sulfate | pH 8.0, 37°C, serum PON1 | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
- |
Homo sapiens | additional information | |
0.0084 | - |
pH 8.0, 37°C, serum PON1 | Homo sapiens | cefazolin | |
0.887 | - |
pH 8.0, 37°C, serum PON1 | Homo sapiens | gentamycin sulfate |