Literature summary for 3.1.3.67 extracted from

Hsu, F.; Zhu, W.; Brennan, L.; Tao, L.; Luo, Z.Q.; Mao, Y.
Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase (2012), Proc. Natl. Acad. Sci. USA, 109, 13567-13572.

Search for more literature for 3.1.3.67

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of SidF and its complex with bound short-chain (dibutanoyl) derivative of PI(3,4)P2 is shown. The structures show that the conserved CX5R catalytic motif is located in a large cationic groove	Legionella pneumophila

Protein Variants

Protein Variants	Comment	Organism
C645S	inactive mutant	Legionella pneumophila
additional information	deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery	Legionella pneumophila
additional information	deletion of the putative transmembrane domains does not affect its enzymatic activity	Legionella pneumophila

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	ectopically expressed GFP-SidF localizes to the ER membrane in mammalian cells. Deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery	Legionella pneumophila	-	-

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila	Q5ZSD5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylinositol 3,4,5-trisphosphate + H2O	-	Legionella pneumophila	phosphatidylinositol 4,5-bisphosphate + phosphate	-	?
phosphatidylinositol 3,4-bisphosphate + H2O	preferred substrate	Legionella pneumophila	phosphatidylinositol 4-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SidF	-	Legionella pneumophila

General Information

General Information	Comment	Organism
malfunction	deletion of SidF results in the reduced recruitment of effector proteins that anchor on the Legionella-containing vacuole (LCV) via binding to PI(4)P	Legionella pneumophila

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Release 2023.1 (January 2023)