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Literature summary for 3.1.3.62 extracted from

  • Cho, J.; King, J.S.; Qian, X.; Harwood, A.J.; Shears, S.B.
    Dephosphorylation of 2,3-bisphosphoglycerate by MIPP expands the regulatory capacity of the Rapoport-Luebering glycolytic shunt (2008), Proc. Natl. Acad. Sci. USA, 105, 5998-6003.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
H89A catalytically compromised mutant of MIPP1 that shows only 1% of the 2,3-diphosphoglycerate phosphatase activity of the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 2,3-bisphosphoglycerate recombinant enzyme, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-
plasma membrane
-
 Rattus norvegicus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 deglycosylated recombinant protein, SDS-PAGE Homo sapiens
55000
-
 deglycosylated recombinant protein, SDS-PAGE Rattus norvegicus
70000
-
 glycosylated recombinant protein, SDS-PAGE Homo sapiens
70000
-
 glycosylated recombinant protein, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Homo sapiens
Ni-Sepharose column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
erythrocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.009
-
 using 2,3-bisphosphoglycerate as substrate, at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-bisphosphoglycerate + H2O
-
 Homo sapiens 2-phosphoglycerate + phosphate
-
 ?
2,3-bisphosphoglycerate + H2O
-
 Rattus norvegicus 2-phosphoglycerate + phosphate
-
 ?
2,3-bisphosphoglycerate + H2O
-
 Dictyostelium discoideum 2-phosphoglycerate + phosphate
-
 ?
inositol 1,3,4,5,6-pentakisphosphate + H2O
-
 Homo sapiens inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?
inositol 1,3,4,5,6-pentakisphosphate + H2O
-
 Rattus norvegicus inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?
inositol 1,3,4,5,6-pentakisphosphate + H2O
-
 Dictyostelium discoideum inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MIPP
-
 Homo sapiens
MIPP
-
 Rattus norvegicus
MIPP
-
 Dictyostelium discoideum
multiple inositol polyphosphate phosphatase
-
 Homo sapiens
multiple inositol polyphosphate phosphatase
-
 Rattus norvegicus
multiple inositol polyphosphate phosphatase
-
 Dictyostelium discoideum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 MIPP1 is active at 4°C, albeit considerably less active than at 37°C Homo sapiens
4 37 MIPP1 is active at 4°C, albeit considerably less active than at 37°C Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.4 MIPP1 activity decreases 50% when pH rises from 7.0 to 7.4 Homo sapiens
7 7.4 MIPP1 activity decreases 50% when pH rises from 7.0 to 7.4 Rattus norvegicus