Cloned (Comment) | Organism |
---|---|
gene ptpA, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D126A | site-directed mutagenesis, mutant PtpA D126A behaves similarly to the PtpA wild-type through the whole expression and purification procedure, suggesting that this mutation does not significantly alter the enzyme structure, but there is a small rearrangement in the protein conformation of the mutant. PtpA wild-type and PtpA D126A mutant present similar values of Km , while the kcat for PtpA D126A is 40fold lower compared to wild-type PtpA, mutant D126A is barely active. PtpA D126A is covalently immobilized on a CM5 sensor chip to assess by surface plasmon resonance (SPR) its ability to bind specifically to macrophage components, SPR analysis of protein-protein interaction, overview | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium ortho-vanadate | - |
Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[a protein]-tyrosine + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
[a protein]-tyrosine + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | Mycobacterium tuberculosis ATCC 25618 | - |
[a protein]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[ATP synthase]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
[ATP synthase]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis ATCC 25618 | - |
[ATP synthase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
[cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis ATCC 25618 | - |
[cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
[sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | Mycobacterium tuberculosis ATCC 25618 | - |
[sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[trifunctional enzyme]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
[trifunctional enzyme]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | Mycobacterium tuberculosis ATCC 25618 | - |
[trifunctional enzyme]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIA1 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WIA1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIA1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [a protein]-tyrosine + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | [a protein]-tyrosine + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [a protein]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [ATP synthase]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | [ATP synthase]-tyrosine + phosphate | - |
? | |
[ATP synthase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [ATP synthase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | [cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [cytosolic 6-phosphofructokinase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | [sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[sulfide quinone oxidoreductase]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [sulfide quinone oxidoreductase]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [trifunctional enzyme]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | also dephosphorylates the human trifunctional enzyme | Mycobacterium tuberculosis | [trifunctional enzyme]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | [trifunctional enzyme]-tyrosine + phosphate | - |
? | |
[trifunctional enzyme]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | [trifunctional enzyme]-tyrosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
probable low molecular weight protein-tyrosine-phosphatase | UniProt | Mycobacterium tuberculosis |
protein tyrosine phosphatase | - |
Mycobacterium tuberculosis |
PtpA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.6 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is active on protein substrates that play a relevant role in cell energy metabolism. PtpA-mediated dephosphorylation may affect pathways involved in cell energy metabolism, particularly the beta oxidation of fatty acids through modulation of TFP activity and/or cell distribution | Mycobacterium tuberculosis |