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Literature summary for 3.1.2.30 extracted from

  • Erb, T.J.; Frerichs-Revermann, L.; Fuchs, G.; Alber, B.E.
    The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)-Malyl-CoA thioesterase (2010), J. Bacteriol., 192, 1249-1258.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
EDTA reversible inhibition Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 (S)-malyl-CoA pH 7.5, 30°C, in the presence of divalent cations (Mg2+ or Mn2+) Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, stimulated by Mg2+ or Mn2+ Cereibacter sphaeroides
Mn2+ 10 mM, stimulated by Mg2+ or Mn2+ Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33400
-
 2 * or 3 * x * 33400, calculated from sequence Cereibacter sphaeroides
80000
-
 gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malyl-CoA + H2O Cereibacter sphaeroides the enzyme is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation (S)-malate + CoA
-
 ?
(S)-malyl-CoA + H2O Cereibacter sphaeroides DSM 158 the enzyme is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation (S)-malate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides D3JV05
-
-
Cereibacter sphaeroides DSM 158 D3JV05
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged proteins Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
 thioester cleavage, pH 7.5, 30°C Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malyl-CoA + H2O the enzyme is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation Cereibacter sphaeroides (S)-malate + CoA
-
 ?
(S)-malyl-CoA + H2O (S)-malyl-CoA i.e. (3S)-3-carboxy-3-hydroxypropanoyl-CoA, the enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 4.1.3.24, malyl-CoA lyase) or other CoA esters Cereibacter sphaeroides (S)-malate + CoA
-
 ?
(S)-malyl-CoA + H2O the enzyme is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation Cereibacter sphaeroides DSM 158 (S)-malate + CoA
-
 ?
(S)-malyl-CoA + H2O (S)-malyl-CoA i.e. (3S)-3-carboxy-3-hydroxypropanoyl-CoA, the enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 4.1.3.24, malyl-CoA lyase) or other CoA esters Cereibacter sphaeroides DSM 158 (S)-malate + CoA
-
 ?

Subunits

Subunits Comment Organism
? 2 * or 3 * x * 33400, calculated from sequence Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
Mcl2
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
110
-
 (S)-malyl-CoA pH 7.5, 30°C Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Cereibacter sphaeroides

General Information

General Information Comment Organism
physiological function the enzyme is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation Cereibacter sphaeroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
 (S)-malyl-CoA pH 7.5, 30°C Cereibacter sphaeroides