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Stimulated by Mg2+ or Mn2+. The enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 22.214.171.124, malyl-CoA lyase) or other CoA esters.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
(S)-malyl-CoA + H2O = (S)-malate + CoA
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