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Literature summary for 3.1.1.7 extracted from

  • Joshi, P.; Singh, B.P.
    Purification and characterization of a cholinesterase from Haemonchus contortus (2000), Indian J. Biochem. Biophys., 37, 192-197.
No PubMed abstract available
Search for more literature for 3.1.1.7

Inhibitors

Inhibitors Comment Organism Structure
acetylcholine iodide substrate inhibition above 4 mM Haemonchus contortus
neostigmine about 60% inhibition at 0.02 mM Haemonchus contortus
phenylmethylsulfonyl fluoride about 60% inhibition at 0.5 mM Haemonchus contortus
physostigmine about 50% inhibition at 0.02 mM Haemonchus contortus
Sodium fluoride about 60% inhibition at 0.5 mM Haemonchus contortus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ omission leads to a 20% decrease in activity Haemonchus contortus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
144000
-
 SDS-PAGE Haemonchus contortus

Organism

Organism UniProt Comment Textmining
Haemonchus contortus
-
 blood-sucking stomach worm of domestic animals
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemonchus contortus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2293
-
-
 Haemonchus contortus

Storage Stability

Storage Stability Organism
4°C, stable for several weeks Haemonchus contortus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine iodide + H2O
-
 Haemonchus contortus acetate + choline iodide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 60% loss of activity after 5 min Haemonchus contortus