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Information on EC 3.1.1.7 - acetylcholinesterase
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3.1.1.7 acetylcholinesteraseIUBMB Comments
Acts on a variety of acetic esters; also catalyses transacetylations.
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Word Map
- 3.1.1.7
-
cholinergic
- alzheimer
- nerve
- butyrylcholinesterase
- cortex
-
organophosphate
-
pesticide
- hippocampus
- fiber
-
insecticide
-
muscarinic
- nervous
-
organophosphorus
- acetyltransferase
- erythrocyte
- deficit
-
histochemical
- cerebral
- dementia
-
neurotoxicity
-
innervation
- donepezil
- synaptic
- atropine
- cholinesterases
-
poison
-
nicotinic
-
neurotransmitter
-
neuroprotective
-
maze
- neuromuscular
-
amyloid
-
histochemistry
- carbamate
- oxime
-
chat
-
intoxication
-
neurochemical
- synapses
-
postsynaptic
- forebrain
- striatum
-
neurotransmission
- chlorpyrifos
- paraoxon
-
morris
-
parasympathetic
- carboxylesterase
-
antidote
- biotechnology
- diagnostics
- analysis
- synthesis
- drug development
- pharmacology
- environmental protection
-
anticholinergic
- medicine
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
AcCholE, ACE, ACE-1, AcE1, ace1a, ace1b, ACE2, ace2-type acetylcholinesterase, ace3, ace4, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AcCholE
-
-
-
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AcE1
ACE2
acetyl beta-methylcholinesterase
-
-
-
-
acetylcholine esterase
acetylcholine hydrolase
acetylcholinesterase
acetylcholinesterase 1
acetylcholinesterase 2
acetylthiocholinesterase
-
-
-
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AChE
AChE1
AChE1A
AChE2
AChEAII
AChEBI
Acylcholine acylhydrolase
ChE
ChE2
choline esterase I
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-
-
-
cholinesterase
esterase, acetyl choline
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-
-
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true cholinesterase
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type VI-S AChE
additional information
acetylcholine esterase
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-
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acetylcholine hydrolase
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AChE
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AChE
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AChE
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AChE
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677724, 677953, 678092, 678096, 678228, 678404, 678608, 679202, 680235, 681108, 681518, 682017, 682920, 682921, 691246, 691746, 691820, 691870, 691944, 692175, 693391, 693553, 694152, 695202, 706828, 707706, 707742, 708092, 708093, 708096, 708206, 708843, 708896, 709298, 709565, 710183, 710187, 710330, 710373, 710620, 710622, 713996, 714110, 714701, 714704, 714852, 715182, 716950, 716958, 728999, 729173, 729380, 729388, 729391, 730993, 730995, 730996, 730997, 749984
AChE
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AChE
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AChE
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AChE
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cholinesterase
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additional information
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the enzyme belongs to the superfamily of alpha/beta-hydrolase fold proteins
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetylcholine + H2O = choline + acetate
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-
-
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acetylcholine + H2O = choline + acetate
mathematical modelling of mechanism
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acetylcholine + H2O = choline + acetate
computer-modelled comparison of ordered uni bi and ki kinetic mechanism. ki model is accurate at equilibrium and when the inhibitor concentration is well below its Kd value
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acetylcholine + H2O = choline + acetate
formation of the trigonal bipyramidal transition state
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acetylcholine + H2O = choline + acetate
reaction mechanism, structure-function relationship analysis, binding structure and mechanism of substrate and product at both the peripheral and active sites, overview
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acetylcholine + H2O = choline + acetate
reaction mechanism, active site structure, the catalytic triad comprises the residues Ser203, His447, Glu334, residues Ser203 and His447 are directly involved in the reaction, serving as nucleophilic attacking group and general acid base catalytic elements, respectively, at the acylation stage of the enzymatic reaction. Modern molecular modeling using tools including molecular docking, molecular dynamics (MD), quantum chemistry and the combined quantum mechanical-molecular mechanical method, overview
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acetylcholine + H2O = choline + acetate
the mechanism for AChE-catalyzed hydrolysis of substrate is formation of the first tetrahedral intermediate via nucleophilic attack of the active site serine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
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transacetylation
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
acetylcholine acetylhydrolase
Acts on a variety of acetic esters; also catalyses transacetylations.
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CAS REGISTRY NUMBER
COMMENTARY
9000-81-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,1-dimethyl-4-acetylthiomethylpiperidinium + H2O
?
-
i.e. MATP+, paper spray mass spectrometry is used to monitor the activity of acetylcholinesterase, both in-solution and on modified hydrophobic paper surface. Hydrophobic paper surfaces are prepared using vaporized trichloro(3,3,3-trifluoropropyl)silane. In both approaches, mixtures of diluted human whole blood with and without inhibitor ethyl ((2-[bis(propan-2-yl)amino]ethyl)sulfanyl)(methyl)phosphonate (VX) are mixed with a non-endogenous AChE specific substrate, 1,1-dimethyl-4-acetylthiomethylpiperidinium (MATP+). Formation of the cleaved MATP+ product is monitored over time and compared to MATP+ to determine relative AChE activity. This on-substrate assay is effective at determining AChE activity and identifying the toxicant, while determination of AChE activity in-solution proceeds at a slower rate. Method evaluation, overview
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
a chromogenic substrate for detection of erythrocyte acetylcholinesterase activity
-
-
?
2,6-dichlorophenolindophenol acetate + H2O
2,6-dichlorophenolindophenol + acetate
-
2,6-dichlorophenolindophenol acetate computational molecular docking study using the enzyme's crystal structure, PDB ID 4YE7
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-
?
3',5'-dichloro-2'-methylindophenylacetate + H2O
3',5'-dichloro-2'-methylindophenol + acetate
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weak substrate
-
?
3',5'-dichlorophenolindophenylacetate + H2O
3',5'-dichlorophenolindophenol + acetate
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weak substrate
-
?
acetyl(beta-methyl)thiocholine iodide + H2O
1-methyl(thio)choline iodide + acetate
-
-
-
-
?
acetyl-(beta-methyl)thiocholine + H2O
(beta-methyl)thiocholine + acetate
-
-
-
?
acetyl-(beta-methyl)thiocholine iodide + H2O
acetate + methylthiocholine iodide
-
-
-
-
?
acetyl-beta-methyl-thiocholine + H2O
beta-methyl-thiocholine + acetate
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-
-
?
acetylthiocholine chloride + H2O
thiocholine chloride + acetate
-
-
-
-
?
alkyl methylphosphonfluoridate + H2O
?
-
oxime-assisted catalysis of organophosphates by AChE, mechanism, reaction system, overview
-
-
?
benzoylthiocholine + H2O
choline + benzoate
-
13% and 9% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively
-
-
?
benzoylthiocholine iodide + H2O
thiocholine iodide + benzoate
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22.2% activity compared to acetylthiocholine
-
-
?
propionylcholine chloride + H2O
choline chloride + propionate
-
-
-
-
?
propionylthiocholine chloride + H2O
thiocholine chloride + propionate
-
-
-
-
?
S-butyrylthiocholine iodide + H2O
thiocholine iodide + butyrate
-
-
-
-
?
2,6-dichloro-3'-methylindophenylacetate + H2O
2,6-dichloro-3'-methylindophenol + acetate
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weak substrate
-
?
2,6-dichloro-3'-methylindophenylacetate + H2O
2,6-dichloro-3'-methylindophenol + acetate
-
-
-
?
2,6-dichlorophenolindophenylacetate + H2O
2,6-dichlorophenolindophenol + acetate
-
-
-
?
2,6-dichlorophenolindophenylacetate + H2O
2,6-dichlorophenolindophenol + acetate
-
-
-
?
2-methoxyacetylthiocholine + H2O
thiocholine + 2-methoxyacetate
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recombinant enzyme shows about 12% activity compared to acetylthiocholine
-
-
?
2-methoxyacetylthiocholine + H2O
thiocholine + 2-methoxyacetate
recombinant enzyme shows about 65% activity compared to acetylthiocholine
-
-
?
3-methylbutyrylthiocholine + H2O
thiocholine + 3-methylbutyrate
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recombinant enzyme shows about 1% activity compared to acetylthiocholine
-
-
?
3-methylbutyrylthiocholine + H2O
thiocholine + 3-methylbutyrate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
acetyl-beta-methylthiocholine + H2O
beta-methylthiocholine + acetate
-
-
-
?
acetyl-beta-methylthiocholine + H2O
beta-methylthiocholine + acetate
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-
-
-
?
acetyl-beta-methylthiocholine + H2O
beta-methylthiocholine + acetate
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-
-
-
?
acetyl-beta-methylthiocholine + H2O
beta-methylthiocholine + acetate
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preferred substrate
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-
?
acetyl-homo-thiocholine + H2O
?
-
recombinant enzyme shows less than 10% activity compared to acetylthiocholine
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-
?
acetyl-homo-thiocholine + H2O
?
recombinant enzyme shows less than 10% activity compared to acetylthiocholine
-
-
?
acetyl-[beta-methyl]thiocholine + H2O
acetate + [beta-methyl]thiocholine
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-
-
-
?
acetyl-[beta-methyl]thiocholine + H2O
acetate + [beta-methyl]thiocholine
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-
-
-
?
acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
?
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synaptic transmission, termination of impulse transmission at cholinergic synapses by hydrolysis
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acetylcholine + H2O
?
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inactivation of transmitter substance operating in cholinergic neurotransmission
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acetylcholine + H2O
acetate + choline
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691156, 692653, 692785, 703783, 707691, 707743, 707747, 708106, 708130, 708139, 708326, 708829, 709593
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-
?
acetylcholine + H2O
acetate + choline
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acetylcholinesterase plays in vivo a key role in cholinergic transmission by catalysing the rapid hydrolysis of the neurotransmitter acetylcholine into acetate and choline
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?
acetylcholine + H2O
acetate + choline
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?
acetylcholine + H2O
acetate + choline
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acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion
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?
acetylcholine + H2O
acetate + choline
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acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion
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?
acetylcholine + H2O
choline + acetate
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-
-
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?
acetylcholine + H2O
choline + acetate
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-
-
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?
acetylcholine + H2O
choline + acetate
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-
-
-
?
acetylcholine + H2O
choline + acetate
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?
acetylcholine + H2O
choline + acetate
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677724, 682017, 713996, 714110, 714704, 716950, 716958, 728999, 729173, 729380, 729388, 729391, 730993, 730995, 730996, 730997, 749984, 716021, 749746, 749759, 750108, 750291, 750310, 750472, 750796, 750919, 750997, 751211, 751284, 751379, 751817, 752242, 752296, 752315
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-
?
acetylcholine + H2O
choline + acetate
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key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate
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?
acetylcholine + H2O
choline + acetate
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the enzyme might be involved in slowing of neurodegeneration in Alzheimer’s and Parkinson’s diseases, overview
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-
?
acetylcholine + H2O
choline + acetate
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esteratic and anionic subsites act together to achieve substrate binding specificity
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?
acetylcholine + H2O
choline + acetate
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-
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?
acetylcholine + H2O
choline + acetate
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94442, 114136, 114150, 114159, 114160, 682128, 682902, 729516, 730995, 749758, 749869, 750070, 752242
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?
acetylcholine + H2O
choline + acetate
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substrate traffic in AChE involves at least two binding sites, the catalytic and peripheral anionic sites, which are allosterically related and involved in substrate inhibition, structure-function relationship analysis, overview
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?
acetylcholine + H2O
choline + acetate
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-
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?
acetylthiocholine + H2O
acetate + thiocholine
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most efficiently hydrolyzed substrate
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-
?
acetylthiocholine + H2O
acetate + thiocholine
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most efficiently hydrolyzed substrate
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-
?
acetylthiocholine + H2O
acetate + thiocholine
-
-
-
-
?
acetylthiocholine + H2O
acetate + thiocholine
-
-
-
-
?
acetylthiocholine + H2O
acetate + thiocholine
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detection using 5,5'-dithiobis(2-nitrobenzoic acid)
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-
?
acetylthiocholine + H2O
acetate + thiocholine
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most efficiently hydrolyzed substrate
-
-
?
acetylthiocholine + H2O
acetate + thiocholine
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most efficiently hydrolyzed substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
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isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1
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-
?
acetylthiocholine + H2O
thiocholine + acetate
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acetylthiocholine iodide
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-
?
acetylthiocholine + H2O
thiocholine + acetate
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-
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
-
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
best substrate for both isozymes AChEA and AChEB
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
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-
664578, 677724, 677953, 678096, 678101, 678228, 678404, 679202, 680235, 681108, 682017, 682920, 682921, 714852, 728999, 729388, 729391, 730993, 730995, 730996, 730997, 749984, 749746, 749759, 750041, 750919, 751379, 752242, 752296
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-
?
acetylthiocholine + H2O
thiocholine + acetate
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activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
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substrate is acetylthiocholine iodide
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
substrate is acetylthiocholine iodide, activity detection using 5,5'-dithio-bis(2-nitrobenzoic acid) colorimetric assay and measurement of the absorbance at 412 nm
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
best substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
best substrate
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
preferred substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
preferred substrate, isozyme PpAChE5 shows the highest catalytic efficiency (Vmax/KM) for substrate acetylthiocholine, which is about 5.4 and 8.6 times compared to butyrylthiocholine and propionylthiocholine, respectively
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
preferred substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
-
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
i.e. acetyl-beta-(methyl)thiocholine iodide (AbMSCh) as substrate
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
catalytic residues are Ser200 and Trp84
-
-
?
acetylthiocholine + H2O
thiocholine + acetate
-
substrate is acetylthiocholine iodide, activity detection using 5,5'-dithio-bis(2-nitrobenzoic acid) colorimetric assay and measurement of the absorbance at 412 nm
-
-
?
acetylthiocholine iodide + H2O
acetate + thiocholine iodide
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
acetylthiocholine iodide + H2O
thiocholine iodide + acetate
-
highly preferred substrate
-
-
?
acetylthiocholine iodide + H2O
thiocholine iodide + acetate
-
preferred substrate
-
-
?
acetylthiocholine iodide + H2O
thiocholine iodide + acetate
-
-
?
benzoylthiocholine + H2O
thiocholine + benzoate
-
recombinant enzyme shows about 1% activity compared to acetylthiocholine
-
-
?
benzoylthiocholine + H2O
thiocholine + benzoate
recombinant enzyme shows about 1% activity compared to acetylthiocholine
-
-
?
butyrylthiocholine + H2O
thiocholine + butanoate
-
-
very bad substrate
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
48% and 41% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
recombinant enzyme shows about 3% activity compared to acetylthiocholine
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
worst substrate
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
native enzyme shows about 50% activity and recombinant enzyme shows about 45% activity compared to acetylthiocholine
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
very low activity
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
-
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
lower activity
-
-
?
butyrylthiocholine iodide + H2O
thiocholine iodide + butyrate
-
low activity
-
-
?
butyrylthiocholine iodide + H2O
thiocholine iodide + butyrate
-
41.7% activity compared to acetylthiocholine
-
-
?
cyclopentylcarbonylthiocholine + H2O
thiocholine + cyclopentanecarboxylate
-
recombinant enzyme shows about 4% activity compared to acetylthiocholine
-
-
?
cyclopentylcarbonylthiocholine + H2O
thiocholine + cyclopentanecarboxylate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
cyclopropylcarbonylthiocholine + H2O
thiocholine + cyclopropanecarboxylate
-
recombinant enzyme shows about 4% activity compared to acetylthiocholine
-
-
?
cyclopropylcarbonylthiocholine + H2O
thiocholine + cyclopropanecarboxylate
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
diisopropyl phosphofluoridate + H2O
?
-
mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme
-
-
?
diisopropyl phosphofluoridate + H2O
?
-
mutant G117H, no activity with the wild-type enzyme
-
-
?
echothiophate + H2O
?
-
mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme
-
-
?
echothiophate + H2O
?
-
mutant G117H, no activity with the wild-type enzyme
-
-
?
hexanoylthiocholine + H2O
thiocholine + hexanoate
-
recombinant enzyme shows about 6% activity compared to acetylthiocholine
-
-
?
hexanoylthiocholine + H2O
thiocholine + hexanoate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
isobutyrylthiocholine + H2O
thiocholine + isobutyrate
-
recombinant enzyme shows about 2% activity compared to acetylthiocholine
-
-
?
isobutyrylthiocholine + H2O
thiocholine + isobutyrate
recombinant enzyme shows about 15% activity compared to acetylthiocholine
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
mutant G117H, no activity with the wild-type enzyme
-
-
?
pentanoylthiocholine + H2O
thiocholine + pentanoate
-
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
pentanoylthiocholine + H2O
thiocholine + pentanoate
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
phenylacetylthiocholine + H2O
thiocholine + phenylacetate
-
recombinant enzyme shows about 7% activity compared to acetylthiocholine
-
-
?
phenylacetylthiocholine + H2O
thiocholine + phenylacetate
recombinant enzyme shows about 3% activity compared to acetylthiocholine
-
-
?
pivaloylthiocholine + H2O
thiocholine + pivalate
-
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
pivaloylthiocholine + H2O
thiocholine + pivalate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
propionylthiocholine + H2O
propanoate + thiocholine
-
preferred over acetylthiocholine
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
71% and 64% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
recombinant enzyme shows about 52% activity compared to acetylthiocholine
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
native and recombinant enzyme show about 70% activity compared to acetylthiocholine
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
good substrate
-
?
propionylthiocholine + H2O
thiocholine + propionate
-
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
low activity
-
-
?
propionylthiocholine iodide + H2O
thiocholine iodide + propionate
-
-
-
?
propionylthiocholine iodide + H2O
thiocholine iodide + propionate
-
preferred substrate
-
-
?
propionylthiocholine iodide + H2O
thiocholine iodide + propionate
-
74.6% activity compared to acetylthiocholine
-
-
?
propionylthiocholine iodide + H2O
thiocholine iodide + propionate
-
-
-
-
?
S-acetylthiocholine + H2O
acetate + thiocholine
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine + H2O
acetate + thiocholine
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine + H2O
acetate + thiocholine
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine + H2O
acetate + thiocholine
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine + H2O
acetate + thiocholine
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB, the enzyme contains a Ser, Glu, and His catalytic triad, overview
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
S-acetylthiocholine iodide + H2O
acetate + thiocholine iodide
-
detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB
-
-
?
additional information
?
-
-
AChE1 is also activity with propionylthiocholine iodide and butyrylthiocholine iodide
-
-
-
additional information
?
-
-
acetylcholinesterase also shows activity with butyrylthiocholine, butyrylcholine, and propionylthiocholine, cf. EC 3.1.1.8
-
-
-
additional information
?
-
-
not: beta-endorphin, angiotensin, oxytoxin, vasopressin
-
-
-
additional information
?
-
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview
-
-
-
additional information
?
-
Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview
-
-
-
additional information
?
-
Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview
-
-
-
additional information
?
-
Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview
-
-
-
additional information
?
-
-
Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview
-
-
-
additional information
?
-
-
isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes
-
-
-
additional information
?
-
isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes
-
-
-
additional information
?
-
isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes
-
-
-
additional information
?
-
active site structure and substrate channels of wild-type and mutant enzymes, overview
-
-
-
additional information
?
-
-
the Dugesia japonica nezyme DjChE has intermediate features of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE, EC 3.1.1.8). Two candidate genes, Djche1 and Djche2, are responsible for DjChE activity
-
-
-
additional information
?
-
-
complex with fasciculin-2 exhibits hydrolytic activity in a buryrylcholinesterase-like kinetics, the switch appears to be a consequence of steric obstruction, but also the consequence of subtle rapid conformational changes around the catalytic site
-
?
additional information
?
-
-
development of an assay method for quantification of the product of enzymatic reaction using substrate as internal standard, thus eliminating the need for addition of an internal standard, mass spectrometric analysis, overview
-
-
-
additional information
?
-
-
activity detection using a capillary electrophoresis, EMMA, method, screening method development and optimization of experimental conditions, overview
-
-
-
additional information
?
-
thiols block the enzymatic etching of gold nanorods(thiocholine on the rate of horseradish peroxidase-catalyzed AuNR etching), application to colorimetric detection of acetylcholinesterase activity and its inhibitors, overview
-
-
-
additional information
?
-
-
lethal effects due to enzyme inhibition in vivo appear at inhibition levels over 50%
-
-
-
additional information
?
-
-
all substrate are used as iodides
-
-
-
additional information
?
-
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
calcium-activated BuchE, EC 3.1.1.8, acts as a major protective mechanism against suicide inhibition of AchE by organophosphates in this non-neuronal tissue, overview
-
-
-
additional information
?
-
-
the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview
-
-
-
additional information
?
-
-
acetylcholinesterase is a serine hydrolase that catalyze the hydrolysis of acetylcholine, thus regulating cholinergic neurotransmission
-
-
-
additional information
?
-
-
inhibition of AChE, along with the butyrylcholinesterase, and restoration of acetylcholine is a therapeutic strategy in treatment of Alzheimer's disease and other forms of dementia
-
-
-
additional information
?
-
-
bioassay for inhibition of fibril formation, i.e. Thioflavin T assay
-
-
-
additional information
?
-
-
the ensemble of compound 1 and compound 2 can be used for the AChE activity assay and the inhibitor-screening by making use of the aggregation-induced emission feature of tetraphenyl ethylene compounds, method development, overview
-
-
-
additional information
?
-
-
interactions between acetylcholinesterase, toxic organophosphorus compounds and a short series of structurally related non-oxime reactivators, analysis of reactivation and inhibition kinetics in vitro, overview
-
-
-
additional information
?
-
-
nonspecific inhibitor neostigmine is used to inhibit hemolysate AChE activity in order to understand the specificity of AChE for 1-naphthyl acetate or acetylthiocholine, substrate docking with AChE, BChE (EC 3.1.1.8), albumin and esterase D, acetylcholine docks with AChE, BChE, albumin and esterase D, overview
-
-
-
additional information
?
-
-
substrate specificities of AChE compared to butyrylcholinesterase (EC 3.1.1.8) and cocaine esterase (EC 3.1.1.84)
-
-
-
additional information
?
-
-
the acetylcholinesterase (AChE) enzyme activity is detected using the 5,5'-dithio-bis(2-nitrobenzoic acid)-based colorimetric assay with measurement of the absorbance at 412 nm. 2,6-Dichloroindophenol acetate is performed as a chromogenic substrate also suitable for AChE activity examination
-
-
-
additional information
?
-
-
activity detection with chromogenic reagent 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB)
-
-
-
additional information
?
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
the enzyme is involved in the morphogenetic processes of neuronal and non-neuronal tissues
-
-
-
additional information
?
-
-
substrate and product trafficking through the active center gorge of the enzyme analyzed by crystallography and equilibrium binding, binding structures, overview
-
-
-
additional information
?
-
-
brain acetylcholinesterase activity controls systemic cytokine levels through the cholinergic anti-inflammatory pathway, overview
-
-
-
additional information
?
-
-
presenilin 1, PS1, is required for enzyme activity in the brain, PS1/A246E transgenic mice have altered AChE activity in several regions also vulnerable in Alzheimer pathology, overview
-
-
-
additional information
?
-
-
Mytilus galloprovincialis ChE degrades preferentially acetylthiocholine (AcSCh), followed by acetyl-beta-methylthiocholine (AbSCh) and propionylthiocholine (PrSCh), while butyrylthiocholine (BuSCh) is poorly hydrolysed. About 9% to 10% of the ChE activity observed, with AcSCh as substrate, is probably due to other esterases (CoEs) activity
-
-
-
additional information
?
-
-
Perforatus perforatus ChE degrades preferentially acetylthiocholine (AcSCh), and acetyl-beta-methylthiocholine (AbSCh), followed by propionylthiocholine (PrSCh), and shows only low activity with butyrylthiocholine (BuSCh)
-
-
-
additional information
?
-
-
Pollicipes pollicipes ChE degrades preferentially propionylthiocholine (PrSCh), followed by acetyl-beta-methylthiocholine (AbSCh) and acetylthiocholine (AcSCh), while butyrylthiocholine (BuSCh) is not hydrolysed
-
-
-
additional information
?
-
-
AChE is an acetylcholine-hydrolyzing enzyme and is implicated in cognitive functions and probably plays important roles in neurodegenerative disorders, including Alzheimer's disease
-
-
-
additional information
?
-
-
acts on a variety of acetic acid esters, also catalyzes transacetylation
-
-
-
additional information
?
-
-
the catalytic triad is formed by His440, Glu327, and Ser200, Trp84 and Trp279 are important for the enzyme activity
-
-
-
additional information
?
-
-
acetylcholinesterase also shows activity with butyrylthiocholine, butyrylcholine, and propionylthiocholine, cf. EC 3.1.1.8
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
?
-
synaptic transmission, termination of impulse transmission at cholinergic synapses by hydrolysis
-
-
-
acetylcholine + H2O
?
-
inactivation of transmitter substance operating in cholinergic neurotransmission
-
-
-
acetylcholine + H2O
acetate + choline
-
-
-
-
?
acetylcholine + H2O
acetate + choline
-
acetylcholinesterase plays in vivo a key role in cholinergic transmission by catalysing the rapid hydrolysis of the neurotransmitter acetylcholine into acetate and choline
-
-
?
acetylcholine + H2O
acetate + choline
-
-
-
-
?
acetylcholine + H2O
acetate + choline
-
acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion
-
-
?
acetylcholine + H2O
acetate + choline
-
acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion
-
-
?
acetylcholine + H2O
choline + acetate
-
-
-
-
?
acetylcholine + H2O
choline + acetate
-
-
-
-
?
acetylcholine + H2O
choline + acetate
-
-
728999, 729173, 729380, 729388, 729391, 730993, 730995, 730996, 730997, 749984, 716021, 749746, 749759, 750108, 750291, 750310, 750472, 750796, 750919, 750997, 751211, 751284, 751379, 751817, 752242, 752296, 752315
-
-
?
acetylcholine + H2O
choline + acetate
-
key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate
-
-
?
acetylcholine + H2O
choline + acetate
-
the enzyme might be involved in slowing of neurodegeneration in Alzheimer’s and Parkinson’s diseases, overview
-
-
?
acetylcholine + H2O
choline + acetate
-
-
-
?
acetylcholine + H2O
choline + acetate
-
-
-
-
?
additional information
?
-
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview
-
-
-
additional information
?
-
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
calcium-activated BuchE, EC 3.1.1.8, acts as a major protective mechanism against suicide inhibition of AchE by organophosphates in this non-neuronal tissue, overview
-
-
-
additional information
?
-
-
the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview
-
-
-
additional information
?
-
-
acetylcholinesterase is a serine hydrolase that catalyze the hydrolysis of acetylcholine, thus regulating cholinergic neurotransmission
-
-
-
additional information
?
-
-
inhibition of AChE, along with the butyrylcholinesterase, and restoration of acetylcholine is a therapeutic strategy in treatment of Alzheimer's disease and other forms of dementia
-
-
-
additional information
?
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
pivotal role in the cholinergic system, rapid termination of nerve impulse transmission
-
?
additional information
?
-
-
the enzyme is involved in the morphogenetic processes of neuronal and non-neuronal tissues
-
-
-
additional information
?
-
-
brain acetylcholinesterase activity controls systemic cytokine levels through the cholinergic anti-inflammatory pathway, overview
-
-
-
additional information
?
-
-
presenilin 1, PS1, is required for enzyme activity in the brain, PS1/A246E transgenic mice have altered AChE activity in several regions also vulnerable in Alzheimer pathology, overview
-
-
-
additional information
?
-
-
AChE is an acetylcholine-hydrolyzing enzyme and is implicated in cognitive functions and probably plays important roles in neurodegenerative disorders, including Alzheimer's disease
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aluminium chloride
-
activates the enzyme in vitro and in vivo after oral treatment
Ca2+
Iron
-
in vitro, iron promotes a significant increase in AChE activity in brain (52%) and liver (53%) at the higher concentration (2.6 mM). In vivo, a significant increase in enzyme activity is observed in the presence of 15 mg/l in both, brain (62%) and liver tissue (70%)
Mg2+
Mn2+
additional information
Mn2+
-
activates isozymes AChEA and AChEB 4.9 and 6.1fold at 1 mM, respectively
additional information
-
no or poor effects by 1 mM of Fe2+, Mn2+, Mg2+, and K+
additional information
-
no or poor effect by Al3+, Ba2+, Mg2+, Mn2+, and Fe2+ at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-(1R,3R,6S)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(+)-(1R,3S,6S)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(+)-(1S,3R,6S)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(+)-(1S,3S,6S)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(+)-huperzine A
-
synthetic enantiomer of the anti-Alzheimer drug (-)-huperzine A and of its natural homologue (-)-huperzine B, interacts with the anionic subsite of the active site
(+)-limonene
-
isolated from the ethanolic extract from the fruits of Pimpinella anisoides
(+)-sabinene
-
isolated from the ethanolic extract from the fruits of Pimpinella anisoides
(-)-(1R,3R,6R)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(-)-(1R,3S,6R)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(-)-(1S,3R,6R)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(-)-(1S,3S,6R)-9-benzyl-3-fluoro-2,4-dioxa-9-aza-3-phosphadecalin 3-oxide
-
-
(-)-3-O-acetyl-spectaline
-
isolated from flowers of Senna spectabilis
(-)-3-O-acetylcassine
-
a semisynthetic alkaloid structure and stereochemistry by ESI-MS/MS and EI-MS