Information on EC 3.1.1.7 - acetylcholinesterase

-

?

-

706828, 707747, 708092, 708093, 708843, 709298, 709565, 710187

-

?

-

acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion

709565

-

?

-

707691, 709565, 709589

-

?

-

acetylcholine protects cardiomyocytes from prolonged hypoxia through activation of the PI3K/Akt/HIF-1alpha/VEGF pathway and that cardiomyocyte-derived VEGF promotes angiogenesis in a paracrine fashion

709565

-

?

Periplaneta americana

-

-

?

-

709296

-

?

Tribolium confusum

-

-

?

Vespula maculifrons

-

-

?

acetylcholine + H2O

choline + acetate

Alca torda

-

682853

-

?

-

-

?

-

-

?

-

730550

-

?

Carassius auratus auratus

-

114147, 114148, 114155, 114159, 114160, 114165, 114172, 683010

-

?

Bungarus multicinctus

-

114145

-

?

-

663746

-

?

Cavia porcellus

-

114134, 716958, 730995, 730996

-

?

Cichla ocellaris

-

729091

-

?

Ciona intestinalis

-

114149

-

?

Crassostrea hongkongensis

-

729567

-

?

Danio rerio

-

729759

-

?

-

114166

-

?

-

114144

-

?

-

-

?

-

683010, 715143, 729388, 729678, 730303

-

?

-

-

?

Fratercula arctica

-

682853

-

?

-

114146, 114156, 114159, 114160, 114164

-

?

-

114133, 114135, 114140, 114154, 114160, 114162, 114163, 114167, 114171

-

?

-

-

?

-

653355

-

653355

?

-

653365

-

653365

?

-

677724, 682017, 713996, 714110, 714704, 716950, 716958, 728999, 729173, 729380, 729388, 729391, 730993, 730995, 730996, 730997, 716021

-

?

-

key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate

677724

-

?

-

the enzyme might be involved in slowing of neurodegeneration in Alzheimer’s and Parkinson’s diseases, overview

682017

-

?

-

fast hydrolysis

714701

-

?

Liposcelis entomophila

-

713976

-

?

Macaca mulatta

-

716958

-

?

-

-

?

-

651529

-

651529

?

-

-

?

-

652016

-

652016

?

-

680690, 729568, 729173

-

?

-

esteratic and anionic subsites act together to achieve substrate binding specificity

-

Polysphondylium violaceum

?

Musca domestica

-

114137, 114138, 114139, 114142

-

?

Narke japonica

-

114169

-

?

-

-

?

-

-

?

Oomyzus sokolowskii

E2FZ90

-

730367

-

?

Oryctolagus cuniculus

-

114170, 730995

-

?

Ovis aries

-

713804

-

?

Pisum sativum

-

114141

-

?

-

114168

-

?

Pseudorasbora parva

-

663746

-

?

-

94442, 114136, 114150, 114159, 114160, 682128, 682902, 729516, 730995

-

?

-

-

?

-

114157

-

?

-

-

?

-

-

?

-

716958, 730995, 730996

-

?

P04058

-

650098

-

650098

?

-

679488

-

?

-

substrate traffic in AChE involves at least two binding sites, the catalytic and peripheral anionic sites, which are allosterically related and involved in substrate inhibition, structure-function relationship analysis, overview

679488

-

?

Torpedo sp.

-

114151, 114152, 114153, 114159, 114160, 114161, 114169

-

?

Trypanosoma evansi

-

729685

-

?

Uria aalge

-

682853

-

?

acetylcholine iodide + H2O

acetate + choline iodide

Trichostrongylus colubriformis

-

114194, 114199

-

?

-

-

?

-

-

?

-

-

-

-

?

-

-

?

-

-

?

-

653994

-

653994

?

-

114193

-

?

Walterinnesia aegyptia

-

114180

-

?

acetylthiocholine + H2O

acetate + thiocholine

Arapaima gigas

-

most efficiently hydrolyzed substrate

-

?

-

710247

-

?

-

most efficiently hydrolyzed substrate

-

?

-

691156, 707743, 708472, 710250

-

?

-

664028, 693391, 706828, 709298, 710620, 710622

-

?

-

detection using 5,5'-dithiobis(2-nitrobenzoic acid)

693553

-

?

-

650742

-

650742

?

-

666973, 690618

-

?

-

666232

-

?

-

most efficiently hydrolyzed substrate

-

?

Rachycentron canadum

-

most efficiently hydrolyzed substrate

-

?

-

708399, 710250

-

?

-

710622

-

?

P04058

-

693390

-

?

Zea mays

-

666633

-

?

acetylthiocholine + H2O

thiocholine + acetate

Apis mellifera

-

isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1

730711

-

?

-

665263, 730550

-

?

P23795

-

114178

-

?

-

114188

-

?

-

-

?

-

680199, 683010

-

?

-

-

?

Cavia porcellus

-

678228, 730995, 730996

-

?

Crassostrea hongkongensis

-

714851

-

?

-

729567

-

?

D2U6X5, D2U6X6

-

-

?

Danio rerio

-

682907

-

?

Q9DDE3

-

682914

-

?

-

729759

-

?

-

-

?

-

-

?

-

-

?

-

664253, 664328, 679202, 682222, 683010, 729388, 729678, 730303

-

?

-

-

?

-

114196

-

?

Gambusia holbrooki

-

665850

-

?

-

649716

-

649716

?

-

-

?

-

-

?

-

650215

-

650215

?

-

-

?

-

653806

-

653806

?

-

664578, 677724, 677953, 678096, 678101, 678228, 678404, 679202, 680235, 681108, 682017, 682920, 682921, 714852, 728999, 729388, 729391, 730993, 730995, 730996, 730997

-

?

-

best substrate

-

?

-

100% activity

-

?

-

fast hydrolysis

714701

-

?

-

666536

-

?

-

best substrate

716835

-

?

100% activity

-

?

Lytechinus variegatus

-

679339

-

?

-

-

?

-

-

?

-

650246

-

650246

?

-

678274, 680690, 681725, 729568

-

?

Mytilus edulis

-

best substrate

-

Mytilus galloprovincialis

?

-

best substrate

-

?

-

-

?

Nephotettix cincticeps Uhler

-

666537

-

?

Nippostrongylus brasiliensis

-

114177, 114198

-

?

Oomyzus sokolowskii

E2FZ90

-

730367

-

?

Oryctolagus cuniculus

-

730995

-

?

Ovis aries

-

114179, 665435, 677887

-

?

Palaemon serratus

-

preferred substrate

663748

-

?

Pisum sativum

-

114141, 664581

-

?

-

653349

-

653349

?

-

679180, 682128, 682902, 716639, 730995

-

?

-

best substrate

-

?

-

-

?

Rhyzopertha dominica

-

114131

-

?

Schistosoma bovis

Q71SU5

-

-

?

Schistosoma haematobium

Q86GL8

-

-

?

Q71SU7

-

-

?

-

-

?

-

-

?

Stomoxys calcitrans

F8QV18

-

716986

-

?

-

730995, 730996

-

?

-

-

?

-

-

?

-

catalytic residues are Ser200 and Trp84

677318

-

?

Trypanosoma evansi

-

729685

-

?

acetylthiocholine iodide + H2O

acetate + thiocholine iodide

Anopheles gambiae

-

708730

-

?

P07140

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

692296

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691742, 692174, 692653

-

?

-

-

?

-

710187

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691870, 692175

-

?

-

-

?

-

709296

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

692179

-

?

acetylthiocholine iodide + H2O

thiocholine + acetate

Cichla ocellaris

-

729091

-

?

-

678092

-

?

acetylthiocholine iodide + H2O

thiocholine iodide + acetate

Blattella germanica

-

715185

-

?

Eisenia andrei

-

679345

-

?

Gammarus pulex

-

highly preferred substrate

Heterorhabditis bacteriophora

-

?

-

preferred substrate

679347

-

?

-

678608

-

?

-

652194

-

652194

?

-

-

?

-

-

?

Q95P20

-

649718

-

649718

?

Oryctolagus cuniculus

-

666538

-

?

-

680122

-

?

-

715396

-

?

-

679488

-

?

alpha-naphthyl acetate + H2O

alpha-naphthol + acetate

Liposcelis bostrychophila

-

114133

-

?

-

665325

-

?

Liposcelis entomophila

-

665325

-

?

aryl-acyl-amides + H2O

?

-

-

?

-

-

?

-

-

?

Torpedo sp.

-

-

?

benzoylthiocholine + H2O

thiocholine + benzoate

-

recombinant enzyme shows about 1% activity compared to acetylthiocholine

-

?

recombinant enzyme shows about 1% activity compared to acetylthiocholine

-

?

butylthiocholine + H2O

thiocholine + butanol

-

-

?

Sitobion avenae

-

-

?

butyrylthiocholine + H2O

butyrate + thiocholine

Arapaima gigas

-

-

?

-

114188

-

?

-

-

?

-

-

?

Nephotettix cincticeps Uhler

-

114196

-

?

Myxine glutinosa

-

114132

-

?

-

666537

-

?

Nippostrongylus brasiliensis

-

114177

-

?

-

-

?

Pisum sativum

-

664581

-

?

Rachycentron canadum

-

-

?

Rhyzopertha dominica

-

114131

-

?

Schistosoma bovis

Q71SU5

-

-

?

Schistosoma haematobium

Q86GL8

-

-

?

Q71SU7

-

-

?

butyrylthiocholine + H2O

thiocholine + butanoate

-

666536

-

?

Palaemon serratus

-

663748

very bad substrate

-

?

butyrylthiocholine + H2O

thiocholine + butyrate

Apis mellifera

-

isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1

730711

-

?

D2U6X5, D2U6X6

-

-

?

-

-

?

-

recombinant enzyme shows about 3% activity compared to acetylthiocholine

-

?

-

worst substrate

716835

-

?

native enzyme shows about 50% activity and recombinant enzyme shows about 45% activity compared to acetylthiocholine

-

?

Lytechinus variegatus

-

679339

-

?

-

-

?

-

680690

-

?

Mytilus edulis

-

-

?

-

666232

-

?

Stomoxys calcitrans

F8QV18

-

716986

-

?

butyrylthiocholine iodide + H2O

thiocholine iodide + butyrate

Blattella germanica

-

715185

-

?

Cyprinus carpio

B7X755

-

694975

-

?

Eisenia andrei

-

679345

-

?

Gammarus pulex

-

low activity

Heterorhabditis bacteriophora

-

?

-

41.7% activity compared to acetylthiocholine

679347

-

?

cyclopentylcarbonylthiocholine + H2O

thiocholine + cyclopentanecarboxylate

-

recombinant enzyme shows about 4% activity compared to acetylthiocholine

-

?

recombinant enzyme shows less than 1% activity compared to acetylthiocholine

-

?

cyclopropylcarbonylthiocholine + H2O

thiocholine + cyclopropanecarboxylate

-

recombinant enzyme shows about 4% activity compared to acetylthiocholine

-

?

recombinant enzyme shows about 5% activity compared to acetylthiocholine

-

?

diisopropyl phosphofluoridate + H2O

?

-

mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme

-

?

-

mutant G117H, no activity with the wild-type enzyme

-

?

echothiophate + H2O

?

-

mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme

-

?

-

mutant G117H, no activity with the wild-type enzyme

-

?

hexanoylthiocholine + H2O

thiocholine + hexanoate

-

recombinant enzyme shows about 6% activity compared to acetylthiocholine

-

?

recombinant enzyme shows less than 1% activity compared to acetylthiocholine

-

?

indophenylacetate + H2O

indophenol + acetate

-

-

?

-

-

?

isobutyrylthiocholine + H2O

thiocholine + isobutyrate

-

recombinant enzyme shows about 2% activity compared to acetylthiocholine

-

?

recombinant enzyme shows about 15% activity compared to acetylthiocholine

-

?

methylthiocholine + H2O

thiocholine + methanol

-

-

?

Sitobion avenae

-

-

?

neuropeptides + H2O

?

-

degradation

-

-

degradation

-

-

degradation

-

Torpedo sp.

-

degradation

-

paraoxon + H2O

?

-

mutant G122H/Y124Q/S125T, no activity with the wild-type enzyme

-

?

-

mutant G117H, no activity with the wild-type enzyme

-

?

pentanoylthiocholine + H2O

thiocholine + pentanoate

-

recombinant enzyme shows about 5% activity compared to acetylthiocholine

-

?

recombinant enzyme shows about 5% activity compared to acetylthiocholine

-

?

phenylacetylthiocholine + H2O

thiocholine + phenylacetate

-

recombinant enzyme shows about 7% activity compared to acetylthiocholine

-

?

recombinant enzyme shows about 3% activity compared to acetylthiocholine

-

?

pivaloylthiocholine + H2O

thiocholine + pivalate

-

recombinant enzyme shows about 5% activity compared to acetylthiocholine

-

?

recombinant enzyme shows less than 1% activity compared to acetylthiocholine

-

?

propionylthiocholine + H2O

propanoate + thiocholine

Arapaima gigas

-

-

?

-

665263

-

?

-

114188

-

?

-

-

?

-

Nephotettix cincticeps Uhler

-

?

-

666537

-

?

-

666232, 729761

-

?

Rachycentron canadum

-

-

?

Rhyzopertha dominica

-

114131

-

?

Zea mays

-

preferred over acetylthiocholine

666633

-

?

propionylthiocholine + H2O

thiocholine + propionate

D2U6X5, D2U6X6

-

-

?

-

-

?

-

recombinant enzyme shows about 52% activity compared to acetylthiocholine

-

?

-

716835

-

?

native and recombinant enzyme show about 70% activity compared to acetylthiocholine

-

?

Mytilus edulis

-

-

Mytilus galloprovincialis

?

-

good substrate

-

?

-

-

?

-

-

?

propionylthiocholine iodide + H2O

thiocholine iodide + propionate

Cyprinus carpio

B7X755

-

694975

-

?

Eisenia andrei

-

preferred substrate

679345

-

?

Gammarus pulex

-

Heterorhabditis bacteriophora

-

?

-

74.6% activity compared to acetylthiocholine

679347

-

?

-

715396

-

?

propylthiocholine + H2O

thiocholine + propanol

-

-

?

Sitobion avenae

-

-

?

S-acetylthiocholine + H2O

acetate + thiocholine

Clytia hemisphaerica

B7ZF08, B7ZF09, B7ZF10

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691408

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691246, 695202

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

690687

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

695151

-

?

S-acetylthiocholine iodide + H2O

acetate + thiocholine iodide

Cyprinus carpio

B7X755

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB, the enzyme contains a Ser, Glu, and His catalytic triad, overview

694975

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

694784

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691820, 694152

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

690686

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

691231

-

?

-

detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB

695200

-

?

S-butyrylthiocholine + H2O

thiocholine + butyrate

-

665263

-

?

-

-

?

additional information

?

-

Anopheles gambiae

-

AChE1 is also activity with propionylthiocholine iodide and butyrylthiocholine iodide

-

-

-

-

not: beta-endorphin, angiotensin, oxytoxin, vasopressin

-

pivotal role in the cholinergic system, rapid termination of nerve impulse transmission

-

?

Cavia porcellus

-

the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview

678228

-

Clytia hemisphaerica

B7ZF08

Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview

-

B7ZF09

Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview

-

B7ZF10

Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview

-

Checx1, Checx2, and Checx3 are three carboxylesterases, that perform the acetylcholineesterase reaction, overview

-

-

isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes

-

D2U6X5

isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes

-

D2U6X6

isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes

-

P07140

active site structure and substrate channels of wild-type and mutant enzymes, overview

692296

-

-

complex with fasciculin-2 exhibits hydrolytic activity in a buryrylcholinesterase-like kinetics, the switch appears to be a consequence of steric obstruction, but also the consequence of subtle rapid conformational changes around the catalytic site

-

?

-

development of an assay method for quantification of the product of enzymatic reaction using substrate as internal standard, thus eliminating the need for addition of an internal standard, mass spectrometric analysis, overview

692785

-

activity detection using a capillary electrophoresis, EMMA, method, screening method development and optimization of experimental conditions, overview

-

-

-

-

-

lethal effects due to enzyme inhibition in vivo appear at inhibition levels over 50%

-

-

114160

-

pivotal role in the cholinergic system, rapid termination of nerve impulse transmission

-

?

-

calcium-activated BuchE, EC 3.1.1.8, acts as a major protective mechanism against suicide inhibition of AchE by organophosphates in this non-neuronal tissue, overview

677953

-

the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview

678228

-

acetylcholinesterase is a serine hydrolase that catalyze the hydrolysis of acetylcholine, thus regulating cholinergic neurotransmission

693553

-

inhibition of AChE, along with the butyrylcholinesterase, and restoration of acetylcholine is a therapeutic strategy in treatment of Alzheimer's disease and other forms of dementia

691944

-

bioassay for inhibition of fibril formation, i.e. Thioflavin T assay

707742

-

the ensemble of compound 1 and compound 2 can be used for the AChE activity assay and the inhibitor-screening by making use of the aggregation-induced emission feature of tetraphenyl ethylene compounds, method development, overview

710187

-

Macaca mulatta

Q67BC1

pivotal role in the cholinergic system, rapid termination of nerve impulse transmission

-

?

Q67BC2

pivotal role in the cholinergic system, rapid termination of nerve impulse transmission

-

?

-

the enzyme is involved in the morphogenetic processes of neuronal and non-neuronal tissues

681725

-

substrate and product trafficking through the active center gorge of the enzyme analyzed by crystallography and equilibrium binding, binding structures, overview

680690

-

brain acetylcholinesterase activity controls systemic cytokine levels through the cholinergic anti-inflammatory pathway, overview

691548

-

presenilin 1, PS1, is required for enzyme activity in the brain, PS1/A246E transgenic mice have altered AChE activity in several regions also vulnerable in Alzheimer pathology, overview

-

-

-

-

AChE is an acetylcholine-hydrolyzing enzyme and is implicated in cognitive functions and probably plays important roles in neurodegenerative disorders, including Alzheimer's disease

693989

-

-

acts on a variety of acetic acid esters, also catalyzes transacetylation

114157

-