Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus islandicus |
Protein Variants | Comment | Organism |
---|---|---|
E14K | Tm-value for the mutant enzyme is 6°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
E14K/Y34Q | Tm-value for the mutant enzyme is 4°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
Y34Q | Tm-value for the mutant enzyme is 8°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.025 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
0.042 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
0.042 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.043 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
0.047 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
0.06 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.061 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
0.1 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.12 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.121 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
0.13 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.16 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.37 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.39 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.44 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.48 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
0.68 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.917 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
1 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
1.2 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
1.3 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
1.9 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
dynamic light scattering | Sulfolobus islandicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus islandicus | C4KKZ9 | - |
- |
Sulfolobus islandicus M.16.4 | C4KKZ9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus islandicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-valerolactone + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
delta-valerolactone + H2O | - |
Sulfolobus islandicus M.16.4 | ? | - |
? | |
dodecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxydodecanoate | - |
? | |
dodecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus M.16.4 | 5-hydroxydodecanoate | - |
? | |
dodecanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | dodecanoate | - |
? | |
epsilon-caprolactone + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
epsilon-caprolactone + H2O | - |
Sulfolobus islandicus M.16.4 | ? | - |
? | |
gamma-butyrolactone + H2O | - |
Sulfolobus islandicus | 4-hydroxybutanoate | - |
? | |
gamma-heptanolide + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
N-(3-oxobutanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxobutanoyl)-DL-homoserine | - |
? | |
N-(3-oxodecanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxodecanoyl)-DL-homoserine | - |
? | |
N-(3-oxododecanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxododecanoyl)-DL-homoserine | - |
? | |
N-(3-oxooctanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxooctanoyl)-DL-homoserine | - |
? | |
N-butanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-butanoyl-L-homoserine | - |
? | |
N-dodecanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-dodecanoyl-DL-homoserine | - |
? | |
N-octanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | octanoyl-homoserine | - |
? | |
nonanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxynonanoate | - |
? | |
nonanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | 4-hydroxynonanoate | - |
? | |
undecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxyundecanoate | - |
? | |
undecanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | 4-hydroxyundecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | at 25°C, dynamic light scattering | Sulfolobus islandicus |
Synonyms | Comment | Organism |
---|---|---|
SisLac | the enzyme also shows activity of aryldialkylphosphatase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2) | Sulfolobus islandicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 84 min | Sulfolobus islandicus |
90 | - |
half-life: 8.5 min | Sulfolobus islandicus |
94 | - |
Tm-value, mutant enzyme Y34Q | Sulfolobus islandicus |
95 | - |
half-life: 3.6 min | Sulfolobus islandicus |
96 | - |
Tm-value, mutant enzyme E14K | Sulfolobus islandicus |
98 | - |
Tm-value, mutant enzyme E14K/Y34Q | Sulfolobus islandicus |
102 | - |
Tm-value, wild-type enzyme | Sulfolobus islandicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.43 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.71 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.85 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
0.92 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
0.97 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
1.5 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
1.9 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
1.9 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
1.9 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
2 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
2.15 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
3.1 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
4.1 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
5.75 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
5.9 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
7.27 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
10.65 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
11.09 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
12.8 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
12.9 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
14.1 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
17.65 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
51.7 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
N-butanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.22 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.76 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
0.9 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
0.93 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
0.95 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
1.05 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
1.46 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
3.14 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
5.49 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
19.8 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
31.3 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
36.4 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
42.3 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
46.1 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
75.2 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
89.5 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
96.3 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
97 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
106 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
204 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
300 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
335 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
828 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus |