Application | Comment | Organism |
---|---|---|
food industry | the enzyme is suitable for both acidic and alkaline processing, such as coffee and tea fermentation | Sphenophorus levis |
Cloned (Comment) | Organism |
---|---|
sequence comparisons, the Sl-pectinase ORF, excluding the coding sequence for signal peptide, is cloned in frame with His-tag into pPICZalphaA and pPICZalphaB expression vectors, recombinant overexpression in Pichia pastoris strain KM71H | Sphenophorus levis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epigallocatechin gallate | exhibits 97.8% inhibition at 1 mg/ml and 47.0% at 0.1 mg/ml | Sphenophorus levis | |
additional information | no inhibition by PME inhibitor from kiwi fruits | Sphenophorus levis | |
NaCl | NaCl causes loss of 40% and 90% of enzyme activity at 0.1 M and 0.5 M, respectively | Sphenophorus levis | |
polyphenon 60 | PP60, exhibits 94.8% inhibition at 1 mg/ml and 26.8% at 0.1 mg/ml | Sphenophorus levis | |
Tannic acid | exhibits 99.5% inhibition at 1 mg/ml and 55.2% at 0.1 mg/ml | Sphenophorus levis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Sphenophorus levis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + n H2O | Sphenophorus levis | - |
n methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphenophorus levis | V9Q624 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme PME contains one N-glycosylation site, deglycosylation of Sl-pectinases is performed using endoglycosidase H (Endo H), peptide-N-glycosidase F (PNGase F) and O-glycosidase at 37°C | Sphenophorus levis |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from pastoris strain KM71H by nickel affinity chromatography | Sphenophorus levis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is active on pectin substrates with degree of methylesterification (DM): sugar beet pectin (DM 42%) apple pectin (DM 70-75%), and citrus pectin (DM 20-34%, 55-70%, and over 85%), but not on polygalacturonic acid | Sphenophorus levis | ? | - |
? | |
pectin + n H2O | - |
Sphenophorus levis | n methanol + pectate | - |
? | |
pectin + n H2O | enzyme Sl-PME shows 100% of relative activity for 85% DM pectin, 51.1% for 55-70% DM pectin, 22.4% for 42% DM pectin and only 6.6% for 20-34% DM pectin | Sphenophorus levis | n methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about, recombinant His-tagged enzyme, SDS-PAGE | Sphenophorus levis |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Sphenophorus levis |
Sl-PME | - |
Sphenophorus levis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Sphenophorus levis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 55 | over 50% of maximal activity at 20-50°C, activity range, profile overview | Sphenophorus levis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 45 | enzyme Sl-PME remains stable up to 35°C, activity decreases by 25% at 40°C and is completely abolished at 45°C | Sphenophorus levis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Sphenophorus levis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | inactive at pH 5.0 and above pH 10.0, over 90% of maximal activity at pH 6.0-8.0 | Sphenophorus levis |
General Information | Comment | Organism |
---|---|---|
physiological function | plant and bacterial pectin methylesterases (PMEs) perform the catalysis with a processive catalytic mechanism, unlike fungal PME whose activity leads to a random repartition of non-esterified carboxyl groups | Sphenophorus levis |