BRENDA - Enzyme Database
show all sequences of 2.8.3.14

Properties of 5-hydroxyvalerate CoA-transferase from Clostridium aminovalericum

Eikmanns, U.; Buckel, W.; Biol. Chem. Hoppe-Seyler 371, 1077-1082 (1990) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
slightly
Anaerocolumna aminovalerica
ATP
-
Anaerocolumna aminovalerica
CTP
-
Anaerocolumna aminovalerica
GTP
slightly
Anaerocolumna aminovalerica
additional information
not inhibited by AMP
Anaerocolumna aminovalerica
Sodium boranate
only in presence of a CoA substrate
Anaerocolumna aminovalerica
UTP
slightly
Anaerocolumna aminovalerica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0038
-
5-hydroxypentanoyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.009
-
propionyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.018
-
acetyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.029
-
butyryl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.03
-
(Z)-5-hydroxypentenoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.037
-
pentanoyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.07
-
butyryl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.1
-
5-hydroxypentanoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.12
-
propionyl-CoA
pH 8.0, 25°C, acetate
Anaerocolumna aminovalerica
0.45
-
pentanoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
4
-
(Z)-5-hydroxy-2-pentenoate
pH 8.0, 25°C, cosubstrate acetyl-CoA
Anaerocolumna aminovalerica
20
-
acetate
pH 8.0, 25°C, cosubstrate propionyl-CoA
Anaerocolumna aminovalerica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
4 * 47000, homotetramer, SDS-PAGE
Anaerocolumna aminovalerica
155000
160000
gradient PAGE
Anaerocolumna aminovalerica
178000
-
average molecular mass
Anaerocolumna aminovalerica
180000
195000
gel filtration, Superose 6
Anaerocolumna aminovalerica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-hydroxypentanoyl-CoA + acetate
Anaerocolumna aminovalerica
-
acetyl-CoA + 5-hydroxypentanoate
-
Anaerocolumna aminovalerica
?
5-hydroxypentanoyl-CoA + acetate
Anaerocolumna aminovalerica T2-7
-
acetyl-CoA + 5-hydroxypentanoate
-
Anaerocolumna aminovalerica T2-7
?
Organism
Organism
UniProt
Commentary
Textmining
Anaerocolumna aminovalerica
-
T2-7
-
Anaerocolumna aminovalerica T2-7
-
T2-7
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Anaerocolumna aminovalerica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38
46
-
Anaerocolumna aminovalerica
Storage Stability
Storage Stability
Organism
-20°C, no loss of activity observed after storage for several months
Anaerocolumna aminovalerica
4°C, lose 50% of its activity within 2 months when kept in 20 mM potassium phosphate buffer, pH 6.8
Anaerocolumna aminovalerica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-hydroxypentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica
?
5-hydroxypentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica T2-7
?
acetate + (Z)-5-hydroxypentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + (Z)-5-hydroxypentenoate
-
-
-
?
acetyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
acetyl-CoA + 3-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + 3-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + 4-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + 4-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica
acetate + 5-hydroxypentanoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
?
acetyl-CoA + propionate
-
643835
Anaerocolumna aminovalerica
acetate + propanoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
butyryl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
butyrate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
butyryl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + butanoate
-
643835
Anaerocolumna aminovalerica
?
butyryl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + butanoate
-
643835
Anaerocolumna aminovalerica T2-7
?
additional information
all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates
643835
Anaerocolumna aminovalerica
?
-
-
-
?
additional information
all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates
643835
Anaerocolumna aminovalerica T2-7
?
-
-
-
?
pentanoyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
pentanoate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
pentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + pentanoate
-
643835
Anaerocolumna aminovalerica
?
propionyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
propionate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
propionyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + propanoate
-
643835
Anaerocolumna aminovalerica
r
propionyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + propanoate
-
643835
Anaerocolumna aminovalerica T2-7
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 47000, homotetramer, SDS-PAGE
Anaerocolumna aminovalerica
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3
-
ATP
pH 8.0, 25°C substrate acetate, non-competitive inhibition
Anaerocolumna aminovalerica
3
-
ATP
pH 8.0, 25°C substrate propionyl-CoA, competitive inhibition
Anaerocolumna aminovalerica
5
-
ATP
pH 8.0, 25°C substrate 5-hydroxypentanoyl-CoA, competitive inhibition
Anaerocolumna aminovalerica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
slightly
Anaerocolumna aminovalerica
ATP
-
Anaerocolumna aminovalerica
CTP
-
Anaerocolumna aminovalerica
GTP
slightly
Anaerocolumna aminovalerica
additional information
not inhibited by AMP
Anaerocolumna aminovalerica
Sodium boranate
only in presence of a CoA substrate
Anaerocolumna aminovalerica
UTP
slightly
Anaerocolumna aminovalerica
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3
-
ATP
pH 8.0, 25°C substrate acetate, non-competitive inhibition
Anaerocolumna aminovalerica
3
-
ATP
pH 8.0, 25°C substrate propionyl-CoA, competitive inhibition
Anaerocolumna aminovalerica
5
-
ATP
pH 8.0, 25°C substrate 5-hydroxypentanoyl-CoA, competitive inhibition
Anaerocolumna aminovalerica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0038
-
5-hydroxypentanoyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.009
-
propionyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.018
-
acetyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.029
-
butyryl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.03
-
(Z)-5-hydroxypentenoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.037
-
pentanoyl-CoA
pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate
Anaerocolumna aminovalerica
0.07
-
butyryl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.1
-
5-hydroxypentanoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
0.12
-
propionyl-CoA
pH 8.0, 25°C, acetate
Anaerocolumna aminovalerica
0.45
-
pentanoyl-CoA
pH 8.0, 25°C, cosubstrate acetate
Anaerocolumna aminovalerica
4
-
(Z)-5-hydroxy-2-pentenoate
pH 8.0, 25°C, cosubstrate acetyl-CoA
Anaerocolumna aminovalerica
20
-
acetate
pH 8.0, 25°C, cosubstrate propionyl-CoA
Anaerocolumna aminovalerica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
4 * 47000, homotetramer, SDS-PAGE
Anaerocolumna aminovalerica
155000
160000
gradient PAGE
Anaerocolumna aminovalerica
178000
-
average molecular mass
Anaerocolumna aminovalerica
180000
195000
gel filtration, Superose 6
Anaerocolumna aminovalerica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-hydroxypentanoyl-CoA + acetate
Anaerocolumna aminovalerica
-
acetyl-CoA + 5-hydroxypentanoate
-
Anaerocolumna aminovalerica
?
5-hydroxypentanoyl-CoA + acetate
Anaerocolumna aminovalerica T2-7
-
acetyl-CoA + 5-hydroxypentanoate
-
Anaerocolumna aminovalerica T2-7
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Anaerocolumna aminovalerica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38
46
-
Anaerocolumna aminovalerica
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, no loss of activity observed after storage for several months
Anaerocolumna aminovalerica
4°C, lose 50% of its activity within 2 months when kept in 20 mM potassium phosphate buffer, pH 6.8
Anaerocolumna aminovalerica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-hydroxypentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica
?
5-hydroxypentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica T2-7
?
acetate + (Z)-5-hydroxypentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + (Z)-5-hydroxypentenoate
-
-
-
?
acetyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
acetyl-CoA + 3-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + 3-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + 4-pentenoate
-
643835
Anaerocolumna aminovalerica
acetate + 4-pentenoyl-CoA
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + 5-hydroxypentanoate
-
643835
Anaerocolumna aminovalerica
acetate + 5-hydroxypentanoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
?
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
?
acetyl-CoA + propionate
-
643835
Anaerocolumna aminovalerica
acetate + propanoyl-CoA
-
643835
Anaerocolumna aminovalerica
r
butyryl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
butyrate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
butyryl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + butanoate
-
643835
Anaerocolumna aminovalerica
?
butyryl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + butanoate
-
643835
Anaerocolumna aminovalerica T2-7
?
additional information
all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates
643835
Anaerocolumna aminovalerica
?
-
-
-
?
additional information
all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates
643835
Anaerocolumna aminovalerica T2-7
?
-
-
-
?
pentanoyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
pentanoate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
pentanoyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + pentanoate
-
643835
Anaerocolumna aminovalerica
?
propionyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
643835
Anaerocolumna aminovalerica
propionate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
-
-
?
propionyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica
acetyl-CoA + propanoate
-
643835
Anaerocolumna aminovalerica
r
propionyl-CoA + acetate
-
643835
Anaerocolumna aminovalerica T2-7
acetyl-CoA + propanoate
-
643835
Anaerocolumna aminovalerica T2-7
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 47000, homotetramer, SDS-PAGE
Anaerocolumna aminovalerica
Other publictions for EC 2.8.3.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643835
Eikmanns
Properties of 5-hydroxyvalerat ...
Anaerocolumna aminovalerica, Anaerocolumna aminovalerica T2-7
Biol. Chem. Hoppe-Seyler
371
1077-1082
1990
-
-
-
-
-
-
7
12
-
-
4
2
-
7
-
-
1
-
-
-
1
2
20
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
7
3
12
-
-
4
2
-
-
-
1
-
-
1
2
20
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-