Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 using the pGEX-2TK expression system | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
17beta-estradiol | - |
Danio rerio | |
0.0235 | - |
estrone | - |
Danio rerio | |
0.0283 | - |
n-propyl gallate | - |
Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Danio rerio | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, SDS-PAGE | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6DHH8 | recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Danio rerio |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0041 | - |
activity with caffeic acid | Danio rerio |
0.0046 | - |
activity with catechin | Danio rerio |
0.0059 | - |
activity with chlorogenic acid | Danio rerio |
0.0066 | - |
activity with p-nitrophenol | Danio rerio |
0.0108 | - |
activity with butylated hydroxy anisole | Danio rerio |
0.0154 | - |
activity with daidzein | Danio rerio |
0.0165 | - |
activity with kaempferol | Danio rerio |
0.0175 | - |
activity with quercetin | Danio rerio |
0.0184 | - |
activity with genistein | Danio rerio |
0.0191 | - |
activity with 17beta-estradiol | Danio rerio |
0.0265 | - |
activity with myricetin | Danio rerio |
0.0283 | - |
activity with epigallocatechin gallate | Danio rerio |
0.0302 | - |
activity with estrone | Danio rerio |
0.0345 | - |
activity with n-propyl gallate | Danio rerio |
0.038 | - |
activity with beta-naphthol | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 17beta-estradiol | - |
Danio rerio | adenosine 3',5'-bisphosphate + estradiol-17beta 3-sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Danio rerio | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + beta-naphthol | - |
Danio rerio | adenosine 3',5'-bisphosphate + 2-naphthyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + butylated hydroxy anisole | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + caffeic acid | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + catechin | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + chlorogenic acid | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + daidzein | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + epigallocatechin gallate | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + estrone | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + genistein | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + kaempferol | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + myricetin | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + n-propyl gallate | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + quercetin | - |
Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
SULT 1 isoform 6 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
2 optima: pH 6.5 and pH 10-10.5, activity with estrone and n-propyl gallate | Danio rerio |
10 | 10.5 | 2 optima: pH 6.5 and pH 10-10.5, activity with estrone and n-propyl gallate | Danio rerio |