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Literature summary for 2.8.2.15 extracted from
- Enzymic synthesis of steroid sulphates. XIII. Isolation and properties of dehydroepiandrosterone sulphotransferase from human foetal adrenals (1980), Biochim. Biophys. Acta, 615, 275-278.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Homo sapiens |
| 68000 | - |
sucrose density gradient ultracentrifugation | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Homo sapiens | 3beta-hydroxyandrost-5-en-17-one | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | foetal | Homo sapiens | - |
| fetus | foetal tissue is a much richer source of enzyme than adult | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0201 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stored in glass ampoules , 0.05 M Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 2% propylene glycol, 1 mg/ml protein, no significant difference in activity observed after storage for 3.5 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 11-deoxycorticosterone | 21-hydroxypregn-4-en-3,20-dione | Homo sapiens | adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate | pregn-4-en-3-one 21-sulfate | r | |
| 3'-phosphoadenylylsulfate + 17alpha-estradiol | estra-1,3,5(10)-triene-3,17alpha-diol | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 17beta-estradiol | estra-1,3,5(10)-triene-3,17alpha-diol | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one | androsterone | Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | androsterone sulfate | r | |
| 3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one | epiandrosterone | Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + androst-5-ene-3beta17alpha-diol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | 3beta-hydroxyandrost-5-en-17-one | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + epitestosterone | 17alpha-hydroxyandrost-4-en-3-one | Homo sapiens | adenosine 3',5'-bisphosphate + epitestosterone sulfate | androst-4-en-3-one 17-sulfate | r | |
| 3'-phosphoadenylylsulfate + estrone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + estrone sulfate | estra-1,3,5(10)-trien-17-one 3-sulfate | r | |
| 3'-phosphoadenylylsulfate + etiocholanone | 3alpha-hydroxy-5beta-androstan-17-one | Homo sapiens | adenosine 3',5'-bisphosphate + etiocholanone sulfate | 5beta-androstan-17-one 3-sulfate | r | |
| 3'-phosphoadenylylsulfate + pregnenolone | 3beta-hydroxypregn-5-en-20-one | Homo sapiens | adenosine 3',5'-bisphosphate + pregnenolone sulfate | pregn-5-en-20-one 3-sulfate | r | |
| 3'-phosphoadenylylsulfate + testosterone | 17beta-hydroxyandrost-4-en-3-one | Homo sapiens | adenosine 3',5'-bisphosphate + testosterone sulfate | androst-4-en-3-one 17-sulfate | r | |
| additional information | little or no activity with pregn-4-ene-11beta,21-diol-3,20-dione/cortisol and oestra-1,3,5(10)-triene-3,17beta-diol-3 methyl ether/17beta-oestradiol-3 methyl ether | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Homo sapiens |
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