Cloned (Comment) | Organism |
---|---|
hexahistidine tagged proteins are expressed in Escherichia coli BL21DE3 pLysS cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D155A | mutation in the conserved YNHNLD sequence | Escherichia coli |
H152A | mutation in the conserved YNHNLD sequence | Escherichia coli |
N151A | mutation in the conserved YNHNLD sequence | Escherichia coli |
N153A | mutation in the conserved YNHNLD sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + S-adenosyl-L-methionine | Escherichia coli | - |
biotin + L-methionine + 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + S-adenosyl-L-methionine | - |
Escherichia coli | biotin + L-methionine + 5'-deoxyadenosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BioB | - |
Escherichia coli |
biotin synthase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | - |
Escherichia coli | |
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | biotin synthase catalyses the final step in the biosynthesis of biotin | Escherichia coli |