Information on EC - biotin synthase

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.1 Sulfurtransferases
       biotin synthase
IUBMB Comments
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5'-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
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Word Map
The enzyme appears in viruses and cellular organisms
Bio2, BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced), BioB, biotin synthase, biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2), biotin synthase (Treponema pallidum gene TP0228), biotin synthetase, biotin synthetase (Aquifex aeolicus gene bioB), biotin synthetase (Arabidopsis thaliana clone pMB101 gene BIO2 reduced), biotin synthetase (Bacillus subtilis gene bioB), more
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
show the reaction diagram
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