BRENDA - Enzyme Database
show all sequences of 2.7.8.6

Biosynthesis of bacterial lipopolysaccharide. VII. Enzymatic formation of the first intermediate in biosynthesis of the O-antigen of Salmonella typhimurium

Osborn, M.J.; Tze-Yuen,R.; J. Biol. Chem. 243, 5145-5152 (1968)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CMP
1 mM: 1% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
dUMP
0.13 mM: 48% inhibition, 0.37 mM: 78% inhibition, 1 mM: 87% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
GMP
1 mM: 5% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
TMP
1.1 mM: 12% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UDP
0.12 mM: no inhibition, 1 mM: 40% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UMP
0.04 mM: 56% inhibition, 0.1 mM: 76% inhibition, 0.4 mM: 93% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UTP
0.11 mM: 5% inhibition, 1 mM: 49% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell envelope
-
Citrobacter sp.
30313
-
cell envelope
-
Salmonella enterica subsp. enterica serovar Typhimurium
30313
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrobacter sp.
-
-
-
Citrobacter sp. 139
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
LT-2, mutant strain G30
-
Storage Stability
Storage Stability
Organism
-18°C, stable for at least 1 month
Salmonella enterica subsp. enterica serovar Typhimurium
-18°C, stable for at least 1 month
Citrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDPgalactose + phospholipid
-
645392
Citrobacter sp.
UMP + galactose-1-diphospho-lipid
-
645392
Citrobacter sp.
r
UDPgalactose + phospholipid
equilibrium constant 0.5
645392
Salmonella enterica subsp. enterica serovar Typhimurium
UMP + galactose-1-diphospho-lipid
-
645392
Salmonella enterica subsp. enterica serovar Typhimurium
r
UDPgalactose + phospholipid
-
645392
Citrobacter sp. 139
UMP + galactose-1-diphospho-lipid
-
645392
Citrobacter sp. 139
r
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CMP
1 mM: 1% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
dUMP
0.13 mM: 48% inhibition, 0.37 mM: 78% inhibition, 1 mM: 87% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
GMP
1 mM: 5% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
TMP
1.1 mM: 12% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UDP
0.12 mM: no inhibition, 1 mM: 40% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UMP
0.04 mM: 56% inhibition, 0.1 mM: 76% inhibition, 0.4 mM: 93% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
UTP
0.11 mM: 5% inhibition, 1 mM: 49% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell envelope
-
Citrobacter sp.
30313
-
cell envelope
-
Salmonella enterica subsp. enterica serovar Typhimurium
30313
-
Storage Stability (protein specific)
Storage Stability
Organism
-18°C, stable for at least 1 month
Salmonella enterica subsp. enterica serovar Typhimurium
-18°C, stable for at least 1 month
Citrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDPgalactose + phospholipid
-
645392
Citrobacter sp.
UMP + galactose-1-diphospho-lipid
-
645392
Citrobacter sp.
r
UDPgalactose + phospholipid
equilibrium constant 0.5
645392
Salmonella enterica subsp. enterica serovar Typhimurium
UMP + galactose-1-diphospho-lipid
-
645392
Salmonella enterica subsp. enterica serovar Typhimurium
r
UDPgalactose + phospholipid
-
645392
Citrobacter sp. 139
UMP + galactose-1-diphospho-lipid
-
645392
Citrobacter sp. 139
r
Other publictions for EC 2.7.8.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722358
Patel
The C-terminal domain of the S ...
Salmonella enterica
Glycobiology
22
116-122
2012
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1
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6
2
1
1
1
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1
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1
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2
1
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1
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722529
Merino
A UDP-HexNAc:polyprenol-P GalN ...
Aeromonas hydrophila, Aeromonas hydrophila AH-3
J. Bacteriol.
193
1943-1952
2011
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1
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1
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4
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8
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722344
Patel
Functional analysis of the C-t ...
Salmonella enterica
Glycobiology
20
1389-1401
2010
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2
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1
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1
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674323
Steiner
Functional characterization of ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NRS 2004/3a
J. Bacteriol.
189
2590-2598
2007
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1
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4
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8
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1
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645392
Osborn
Biosynthesis of bacterial lipo ...
Citrobacter sp. 139, Citrobacter sp., Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
243
5145-5152
1968
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2
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3
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3
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645391
Wright
Characterization of a polyisop ...
Salmonella newington
Proc. Natl. Acad. Sci. USA
57
1798-1803
1967
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