minimal activity is detected with the shorter polyisoprenyl phosphates like C10-P, C15-P, C20-P and C35-P at 0.28, 0.91, 1 and 1.8% of C55-undecaprenyl phosphate activity, respectively. C50-phosphate, however, results in 49.53% activity of C55-phosphate
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from Escherichia coli BL21(DE3) Star and TOP10 membrane preparations, nickel affinity chromatography (batch method), in presence of 0.1 mM lauryldimethylamine N-oxide; His-Select nickel affinity gel column chromatography
expressed in Escherichia coli TOP10 and BL21(DE3) Star cells, and in Salmonella enterica serovar Typhimurium; from genomic DNA, (i) in pET28a for inducible expression of entire coding sequence or C-terminal part with N-terminal hexa-His-tag and subcellular localisation in Escherichia coli BL21(DE3) Star, (ii) in pBAD-DEST49: for inducible expression with N-terminal horseradish peroxide-thioredoxin-tag and C-terminal hexa-His-tag in Escherichia coli TOP10, (iii) in pBluescript II SK(+): for complementation studies with full-length WsaP, C-terminal part (aa168-417), N-terminal part (aa1-314) or a mutant lacking the transmembrane domains (aa311-471) in Escherichia coli strain CWG466 and Salmonella enterica strain MSS2