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Literature summary for 2.7.7.74 extracted from
- Bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase, the key enzyme for di-myo-inositol-phosphate synthesis in several (hyper)thermophiles (2007), J. Bacteriol., 189, 5405-5412.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
- |
Thermococcus kodakarensis |
- |
Aquifex aeolicus |
- |
Rubrobacter xylanophilus |
| expression in Escherichia coli | Archaeoglobus fulgidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 1L-myo-inositol 1-phosphate | Archaeoglobus fulgidus | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? |  |
| CTP + 1L-myo-inositol 1-phosphate | Pyrococcus furiosus | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | Thermococcus kodakarensis | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | Aquifex aeolicus | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | Rubrobacter xylanophilus | the enzyme is involved in biosynthesis of di-myo-inositol 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O67379 | - |
- |
| Archaeoglobus fulgidus | O29976 | - |
- |
| Pyrococcus furiosus | Q8U1Z6 | - |
- |
| Rubrobacter xylanophilus | Q1AWQ0 | - |
- |
| Thermococcus kodakarensis | Q5JDA9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Archaeoglobus fulgidus | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Pyrococcus furiosus | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Thermococcus kodakarensis | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Aquifex aeolicus | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of di-myo-inositol 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Rubrobacter xylanophilus | diphosphate + CDP-1L-myo-inositol | - |
? | |
| CTP + 1L-myo-inositol 1-phosphate | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors | Archaeoglobus fulgidus | diphosphate + CDP-1L-myo-inositol | structural characterization of products by nuclear magnetic resonance | ? | |
| CTP + 1L-myo-inositol 1-phosphate | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors | Pyrococcus furiosus | diphosphate + CDP-1L-myo-inositol | structural characterization of products by nuclear magnetic resonance | ? | |
| CTP + 1L-myo-inositol 1-phosphate | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors | Thermococcus kodakarensis | diphosphate + CDP-1L-myo-inositol | structural characterization of products by nuclear magnetic resonance | ? | |
| CTP + 1L-myo-inositol 1-phosphate | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors | Aquifex aeolicus | diphosphate + CDP-1L-myo-inositol | structural characterization of products by nuclear magnetic resonance | ? | |
| CTP + 1L-myo-inositol 1-phosphate | no CDP-inositol:inositol-1-phosphate transferase activity detected. myo-Inositol is not a substrate | Rubrobacter xylanophilus | diphosphate + CDP-1L-myo-inositol | structural characterization of products by nuclear magnetic resonance | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CTP:inositol-1-phosphate cytidylyltransferase | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Archaeoglobus fulgidus |
| CTP:inositol-1-phosphate cytidylyltransferase | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Pyrococcus furiosus |
| CTP:inositol-1-phosphate cytidylyltransferase | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Thermococcus kodakarensis |
| CTP:inositol-1-phosphate cytidylyltransferase | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Aquifex aeolicus |
| CTP:inositol-1-phosphate cytidylyltransferase | no CDP-inositol:inositol-1-phosphate transferase activity detected | Rubrobacter xylanophilus |
| IPCT | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Archaeoglobus fulgidus |
| IPCT | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Pyrococcus furiosus |
| IPCT | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Thermococcus kodakarensis |
| IPCT | bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS) | Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Archaeoglobus fulgidus |
| 80 | - |
assay at | Pyrococcus furiosus |
| 80 | - |
assay at | Thermococcus kodakarensis |
| 80 | - |
assay at | Aquifex aeolicus |
| 80 | - |
assay at | Rubrobacter xylanophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Archaeoglobus fulgidus |
| 7.6 | - |
assay at | Pyrococcus furiosus |
| 7.6 | - |
assay at | Thermococcus kodakarensis |
| 7.6 | - |
assay at | Aquifex aeolicus |
| 7.6 | - |
assay at | Rubrobacter xylanophilus |
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