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Literature summary for 2.7.7.48 extracted from
- Arterivirus RNA-dependent RNA polymerase: vital enzymatic activity remains elusive (2016), Virology, 487, 68-74.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His-tagged RNA-dependent RNA polymerase (RdRp) as the C-terminal domain of nonstructural protein 9 (nsp9) in Escherichia coli strain BL21 (DE3) | equine arteritis virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | equine arteritis virus |  |
| Mn2+ | essentially required for catalytic activity | equine arteritis virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| equine arteritis virus | - |
an arterivirus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged RdRp as the C-terminal domain of nonstructural protein 9 from Escherichia coli strain BL21 (DE3) by cobalt affinity chromatography and gel filtration | equine arteritis virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | in vitro, protein nsp9 of equine arteritis virus shows weak de novo polymerase activity on homopolymeric RNA templates. The RNA-synthesizing activities observed in de novo and primer-dependent polymerase and terminal transferase assays cannot be attributed to recombinant EAV nsp9-RdRp. The polymerase assay employing 32P-labeled NTPs is sensitive enough to detect the activity of trace amounts of contaminating T7 RNA polymerase. This polymerase is also able to act on templates lacking the established T7 promoter requirements | equine arteritis virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RDRP | - |
equine arteritis virus |
| RNA-dependent RNA polymerase | - |
equine arteritis virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | equine arteritis virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | equine arteritis virus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the RNA-dependent RNA polymerase (RdRp) in equine arteritis virus is expressed as the C-terminal domain of nonstructural protein 9 (nsp9) | equine arteritis virus |
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