Literature summary for 2.7.7.1 extracted from

Konishi, K.; Ueda, S.; Kawano, M.; Osawa, S.; Tamura, T.; Hokazono, E.; Kayamori, Y.; Sakasegawa, S.I.
Characterization and application of a novel nicotinamide mononucleotide adenylyltransferase from Thermus thermophilus HB8 (2018), J. Biosci. Bioeng., 125, 385-389 .

Search for more literature for 2.7.7.1

Application

Application	Comment	Organism
analysis	NMNAT can be applied to the colorimetric NMN or NaMN assays, which employ either adenylation of NMN to NAD by NMNAT or adenylation of NaMN to deamido-NAD (NaAD) by NMNAT followed by amidation of NaAD to NAD by NAD synthetase (NADS, EC 6.3.1.5) or an NAD cycling reaction using 12alpha-hydroxysteroid dehydrogenase (12a-HSD, EC 1.1.1.176) and diaphorase (DI, EC 1.6.99.3) to accumulate reduced 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium. The enzymatic cycling method enables detection of 0.5 mM (12.2 nM in the reaction mixture) NMN or NaMN in an automatic clinical analyzer	Thermus thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.263	-	nicotinamide ribonucleotide	pH 8.0, 37°C	Thermus thermophilus
1.27	-	ATP	pH 8.0, 37°C	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	Ni2+ supports enzyme activity most efficiently, followed by Co2+, Mg2+, and Mn2	Thermus thermophilus
Mg2+	Ni2+ supports enzyme activity most efficiently, followed by Co2+, Mg2+, and Mn2	Thermus thermophilus
Mn2+	Ni2+ supports enzyme activity most efficiently, followed by Co2+, Mg2+, and Mn2	Thermus thermophilus
Ni2+	Ni2+ supports enzyme activity most efficiently, followed by Co2+, Mg2+, and Mn2	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
77800	-	gel filtration	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q5SHF0	cf. EC 2.7.7.18	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	-	Thermus thermophilus	diphosphate + NAD+	-	?
additional information	no donors: ADP, AMP, TMP, GMP, CMP, UMP and IMP	Thermus thermophilus	?	-	-

Subunits

Subunits	Comment	Organism
trimer	3 * 22000, SDS-PAGE, x * 21051, calculated from sequence	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
TTHA1780	-	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	45 min, no loss of activity	Thermus thermophilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermus thermophilus	calculated from sequence	-	6.5

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Release 2023.1 (January 2023)