Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAPII) facilitate RNA polymerase II CTD dephosphorylation | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
T162A | the kin28-T162A allele, which does not display a slow growth phenotype, has reduced activity in vitro, but does not detectably affect CTD phosphorylation and capping enzymes recruitment in vivo | Saccharomyces cerevisiae |
T17D | the mutant has decreased CTD kinase activity, a defect in capping enzyme recruitment, and is slow growing at 25°C and 37°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatin | - |
Saccharomyces cerevisiae | 785 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | ADP + phospho-[DNA-directed RNA polymerase] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P06242 | - |
- |
Saccharomyces cerevisiae | P39073 | - |
- |
Saccharomyces cerevisiae | Q03957 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [DNA-directed RNA polymerase] | CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTD kinase | - |
Saccharomyces cerevisiae |
Ctk1 | - |
Saccharomyces cerevisiae |
Kin28 | - |
Saccharomyces cerevisiae |
Ser5/Ser7 kinase | - |
Saccharomyces cerevisiae |
Srb10 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphorylation state of the C-terminal domain of RNA polymerase II, detailed overview | Saccharomyces cerevisiae |
physiological function | Ctk1, the main Ser2 kinase in coding and 3'-end regions, is required for cotranscriptional recruitment of the polyadenylation machinery. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants | Saccharomyces cerevisiae |
physiological function | Kin28 (mammalian Cdk7), a Ser5/Ser7 kinase, facilitates transcription and formation of the scaffold complex together with Ser2/Ser5 kinase Srb10 (mammalian Cdk8). Kin28 is part of the TFIIH initiation complex and phosphorylates Ser5 to promote cotranscriptional 5' mRNA capping, and in mammals also phosphorylates Ser7 during promoter-proximal pausing and perhaps during termination. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants | Saccharomyces cerevisiae |
physiological function | Srb10 (mammalian Cdk8), a Ser2/Ser5 kinase, is part of the Mediator complex and inactivatesRNAPII prior to pre-initiation complex formation, and, together with Kin28 (a Ser5/Ser7 kinase), facilitates transcription and formation of the scaffold complex. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants | Saccharomyces cerevisiae |