Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a bur1 knockout mutant bur1DELTA | Saccharomyces cerevisiae |
additional information | generation of a ctk1 knockout mutant ctk1DELTA | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity of CTD kinase Bur1 is repressed in the presence of a functional CTD kinase Kin28 complex | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | profiling of the location of the RNAPII phosphorylated isoforms in wild-type cells and mutants for most CTD modifying enzymes. The CTD cycle is uniform across genes | Saccharomyces cerevisiae | - |
- |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | - |
ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | Ctk1 is the major serine 2 kinase in vivo | ADP + phospho-[DNA-directed RNA polymerase] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P06242 | - |
- |
Saccharomyces cerevisiae | P23293 | - |
- |
Saccharomyces cerevisiae | Q03957 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + C-terminal domain of RNA polymerase II | the recombinant GST-tagged CTD substrate is hyperphosphorylated by Kin28 | Saccharomyces cerevisiae | ADP + phosphorylated C-terminal domain of RNA polymerase II | - |
? | |
ATP + [DNA-directed RNA polymerase] | - |
Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | Ctk1 is the major serine 2 kinase in vivo | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | in vitro, Ctk1 complex can phosphorylate the CTD at all three serines, although with different efficiencies. Ctk1 phosphorylates Ser2 in vitro | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | phosphorylation of Ser2, Ser5, and Ser7 in CTD | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
ATP + [DNA-directed RNA polymerase] | phosphorylation of Ser5 and Ser7 in CTD, Bur1 phosphorylates Ser2 in vitro | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bur1 | - |
Saccharomyces cerevisiae |
CTD kinase | - |
Saccharomyces cerevisiae |
Ctk1 | - |
Saccharomyces cerevisiae |
Kin28 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the universal RNA polymerase II CTD cycle is orchestrated by complex interplays between kinase, phosphatase, and isomerase enzymes along genes | Saccharomyces cerevisiae |
physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Bur1 is a potent Ser5 and Ser7 kinase | Saccharomyces cerevisiae |
physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Enzyme Ctk1 does not play any significant role in maintaining P-Ser5 and P-Ser7 levels in wild-type cells, the phosphorylation activity versus Ser5 and Ser7 is mainly in an opportunistic manner. Ctk1 is the major serine 2 kinase during transcription in vivo | Saccharomyces cerevisiae |
physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Kin28 is the main CTD kinase. sThe kinase activity of Kin28 downregulates the activity of Bur1 against P-Ser5 and 7 along the coding region | Saccharomyces cerevisiae |