Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclin T1 | is in a complex with cdk9 and forms P-TEFb | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | IE2 and multiple cellular factors needed for viral RNA synthesis accumulate within the first 8 h at the viral transcriptosome, functional cdk activity is required for the specific recruitment of cdk7 and cdk9 during this time interval | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA copy of cdk9HA placed into a lentiviral vector where its transcription is driven by an EF1alpha promoter, overexpression in HFF cells | Homo sapiens |
overexpression of HA-tagged cdk9 in 293FT cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
roscovitine | impairment in the localization of proteins to the immediate-early transcription sites during roscovitine treatment is specific to cdk9 and cdk7, decreases levels of cdk9 and cdk7 RNA, a corresponding reduction in cdk9 protein but only a modest decrease in cdk7 protein, a delay in the addition of roscovitine until 8 h p.i. does not inhibit cdk9 and cdk7 protein expression in human cytomegalovirus-infected cells, overexpression of cdk9 does not compensate for the effects of roscovitine on its localization in human cytomegalovirus-infected cells; inhibition of cdk7 expression during roscovitine treatment occurs at the RNA level; inhibition of cdk9 expression during roscovitine treatment occurs at the RNA level, overexpression of cdk9 does not compensate for the effects of roscovitine on its localization in HCMV-infected cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | localization of cdk7 and cdk9 to the viral transcriptosome of human cytomegalovirus | Homo sapiens | - |
- |
additional information | human cytomegalovirus, HCMV, infection in the presence of roscovitine impairs the localization of cdk7, but not of IE2-86, at viral transcriptosomes | Homo sapiens | - |
- |
additional information | human cytomegalovirus, HCMV, infection in the presence of roscovitine impairs the localization of cdk9, but not of IE2-86, at viral transcriptosomes | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
transcriptosome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA polymerase II C-terminal subunit | Homo sapiens | i.e. RNAP II CTD, hypophosphorylated form of RNAP IIa is recruited to the preinitiation complex at the gene promoter by the general transcription factors. Initiation proceeds when the cdk7 complex phosphorylates the CTD at the serine 5 residues, resulting in a hyperphosphorylated RNAP II that recruits the RNA-capping enzymes, overview | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
additional information | Homo sapiens | cdk7 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview | ? | - |
? | |
additional information | Homo sapiens | cdk9 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P50613 | cdk7 | - |
Homo sapiens | P50750 | cdk9 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | of foreskin | Homo sapiens | - |
additional information | HFF cell | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA polymerase II | both cdk7 and cdk9 are responsible for phosphorylation at the C-terminal domain | Homo sapiens | ? | - |
? | |
ATP + RNA polymerase II C-terminal subunit | i.e. RNAP II CTD, hypophosphorylated form of RNAP IIa is recruited to the preinitiation complex at the gene promoter by the general transcription factors. Initiation proceeds when the cdk7 complex phosphorylates the CTD at the serine 5 residues, resulting in a hyperphosphorylated RNAP II that recruits the RNA-capping enzymes, overview | Homo sapiens | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
ATP + RNA polymerase II C-terminal subunit | i.e. RNAP II CTD, phosphorylation at Ser5 | Homo sapiens | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
additional information | cdk7 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview | Homo sapiens | ? | - |
? | |
additional information | cdk9 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cdk7 | - |
Homo sapiens |
CDK9 | - |
Homo sapiens |
RNA polymerase II carboxyl-terminal domain kinase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |