Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
gene Myl12b, analysis of MLCK, L-type (CaV1.2), P/Q-type (CaV2.1), and T-type (CaV3.1) voltage-dependent Ca2+ channels and RPL32 mRNA expression by real-time quantitative-PCR | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | MLCK is downregulated by transfection with a small interfering RNA targeting MLCK (MLCK-siRNA) in rat small resistance mesenteric artery and in the rat embryonic aortic cell line A7r5. Expression of long (220 kDa) and short (130 kDa) MLCK is inhibited in MLCK-siRNA-transfected cells. Noradrenaline-induced contraction and Ca2+ signal are significantly depressed in MLCK-siRNA compared to scramble-siRNA-transfected small resistance mesenteric artery. Contraction and Ca2+ signal induced by high KCl and voltage-activated Ca2+ current are also significantly decreased in MLCK-siRNAtransfected resistance mesenteric artery | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ML-7 | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, activates | Rattus norvegicus | |
Mg2+ | required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
short MLCK isozyme | Rattus norvegicus |
220000 | - |
long MLCK isozyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | Rattus norvegicus | smooth muscle myosin | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | Rattus norvegicus Wistar | smooth muscle myosin | ADP + [myosin light chain] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | D3ZFU9 | - |
- |
Rattus norvegicus Wistar | D3ZFU9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A7r5 cell | an embryonic thoracic aorta cell line | Rattus norvegicus | - |
artery | small resistance mesenteric artery | Rattus norvegicus | - |
smooth muscle | long (220 kDa) and short (130 kDa) MLCK isozymes | Rattus norvegicus | - |
vascular smooth muscle cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | smooth muscle myosin | Rattus norvegicus | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | smooth muscle myosin | Rattus norvegicus Wistar | ADP + [myosin light chain] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ca2+-dependent kinase myosin light chain kinase | - |
Rattus norvegicus |
MLCK | - |
Rattus norvegicus |
myosin light chain kinase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | MLCK depletion modifies voltage-operated Ca2+ channels. KCl- and AVP-induced Ca2+ signals and voltage-activated Ca2+ current are decreased in MLCK-depleted A7r5 cells. MLCK enzyme inhibition leads to a significant inhibition of cytosolic Ca2+ increase (64%) and contraction induced by 50 mM KCl (92%). Phenotype, detailed overview. Voltage-dependent Ca2+ channel gene expression is decreased by MLCK downregulation | Rattus norvegicus |
physiological function | stimulation of vascular smooth muscle cells increases cytosolic Ca2+ concentration as a result of Ca2+ entry from the extracellular space through plasma membrane Ca2+ channels and/or Ca2+ release from intracellular stores via the sarcoplasmic reticulum Ca2+ channels. The increase in Ca2+/calmodulin complex activates myosin light chain kinase (MLCK) triggering contraction, which regulates vasoactivity and blood flow | Rattus norvegicus |