Literature summary for 2.7.11.18 extracted from

Wadgaonkar, R.; Nurmukhambetova, S.; Zaiman, A.L.; Garcia, J.G.
Mutation analysis of the non-muscle myosin light chain kinase (MLCK) deletion constructs on CV1 fibroblast contractile activity and proliferation (2003), J. Cell. Biochem., 88, 623-634.

Search for more literature for 2.7.11.18

Cloned(Commentary)

Cloned (Comment)	Organism
expression of calmodulin-independent mutant MLCK1745 and of MLVKATPdel mutant in African green monkey kidney CV1 fibroblasts as V5-epitope- and His-tagged proteins	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A27F/A29R/A31R/A32F	mutant enzyme with defective ATP binding site, mutant leads to nearly unaltered MLC phosphorylation activity and altered morphology in transfected CV1 cells	Homo sapiens
additional information	construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutation leads to increased growth rates of transfected CV1 fibroblasts and an increase in basal level MLC phosphorylation compared to untransfected cells, stress fiber formation is unaffected by the mutation	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
190000	-	x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE	Homo sapiens
210000	-	x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [myosin light chain]	Homo sapiens	-	ADP + [myosin light chain] phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
endothelial cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [myosin light chain]	-	Homo sapiens	ADP + [myosin light chain] phosphate	-	?
ATP + [myosin light chain]	activated phosphorylated enzyme	Homo sapiens	ADP + [myosin light chain] phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
EC MLCK	-	Homo sapiens
nmMLCK	-	Homo sapiens
non-muscle myosin light chain kinase	-	Homo sapiens
smMLCK	-	Homo sapiens
smooth-muscle myosin light chain kinase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	residues A27, A29, A32, and A31 are involved in binding	Homo sapiens
Calmodulin	-	Homo sapiens

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Release 2023.1 (January 2023)