Cloned (Comment) | Organism |
---|---|
co-overexpression of GST-tagged enzyme with GST-tagged Whi3 protein in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [Whi3 cell cycle regulator protein] | Saccharomyces cerevisiae | phosphorylation of Ser568 leads to inhibition of Whi3 | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme and GST-tagged Whi3 protein from Saccharomyces cerevisiae by glutathione affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [Whi3 cell cycle regulator protein] | phosphorylation of Ser568 leads to inhibition of Whi3 | Saccharomyces cerevisiae | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? | |
ATP + [Whi3 cell cycle regulator protein] | phosphorylation of Ser568 | Saccharomyces cerevisiae | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Saccharomyces cerevisiae |
Ras/cAMP-dependent protein kinase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
additional information | the phosphomimetic S568D mutation of Whi3 prevents the developmental fate switch to sporulation or invasive growth | Saccharomyces cerevisiae |
physiological function | phosphorylation of Whi3 by PKA leads to its decreased interaction with CLN3 G1 cyclin mRNA and is required for the promotion of G1/S progression, phosphorylation state of Ser568 in Whi3 affects G1/S transition by modulating CLN2 transcription. PKA causes a decrease in cell size by downregulating Whi3 function. Implication of PKA-mediated modulation of Whi3 in multiple cellular events | Saccharomyces cerevisiae |