Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant GST-tagged MPK38 in human in MCF-7, HEK-293, H-1299, and HCT-116 cells, coexpression with FLAG-tagged p53 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of kinase-dead Mpk38 and enzyme knockout by Mpk38-specific siRNA | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs autophosphorylation | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + p53 protein | at Ser15 | Mus musculus | ADP + phosphorylated p53 protein | - |
? | |
additional information | the enzyme performs autophosphorylation | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPK38 | - |
Mus musculus |
murine protein serine-threonine kinase 38 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
evolution | MPK38 is a member of the AMPK family | Mus musculus |
physiological function | MPK38 is functionally linked to the p53 signaling pathway. MPK38 directly associates with p53 without relying on additional proteins in mammalian cells. The C-terminal regulatory domain, residues 270-643, of MPK38 is responsible for p53 binding, while the N-terminal domain is unable to bind p53. Binding is mediated by the the DNA-binding domain and the basic domain, not by the transactivation domain, of p53, MPK38 interaction with p53 deletion mutants, overview. MPK38 acts as a positive regulator of p53, while expression of MPK38 itself has little effect on p53-induced transcription. MPK38 upregulates the expression of p53 targets in a kinase-dependent manner, and it promotes translocation of p53 from cytosol to the nucleus, overview. MPK38 stimulates p53-induced apoptosis and cell cycle arrest | Mus musculus |