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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase
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EC Tree
2.7.11.1 non-specific serine/threonine protein kinaseIUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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Word Map
- 2.7.11.1
- jnk
- mapks
-
signal-regulated
- agonist
- cardiac
- rapamycin
- necrosis
- endothelial
- parkinsonism
- mtor
- synaptic
- cyclase
- forskolin
- bcl-2
- cyclin
- caspase-3
- metastasis
- alzheimer
- adhesion
- hippocampal
- sirnas
- neurodegenerative
-
phorbol
- autophosphorylation
- myocytes
- cardiomyocytes
- tumorigenesis
- creb
-
neuroprotective
- beta-catenin
-
adenylyl
- ventricular
- phosphoprotein
- wortmannin
- nf-kappab
-
cyclin-dependent
- pten
- dopamine
- ischemia
- gtpases
- src
- nmda
-
hyperphosphorylation
-
dominant-negative
-
anti-apoptotic
-
postsynaptic
-
l-type
- isoproterenol
- staurosporine
-
tensin
- drug development
- medicine
- molecular biology
- pharmacology
The enzyme appears in viruses and cellular organisms
Synonyms
protein kinase a, 14-3-3, camkii, c-jun n-terminal kinase, p-akt, lrrk2, raf-1, serine/threonine kinase, protein kinase b, gsk-3, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3-phosphoinositide dependent protein kinase-1
30 kDa protein kinase homolog
5'-AMP-activated protein kinase, catalytic alpha-1 chain
5'-AMP-activated protein kinase, catalytic alpha-2 chain
AKT
AKT1
AKT2
Akt3
AMPK
AsfK
BA-Stk1
calcium-dependent protein kinase
CaMKII
carbon catabolite derepressing protein kinase
casein kinase
casein kinase 1
casein kinase 1delta
casein kinase 1gamma
casein kinase 2
casein kinase 2 alpha4
casein kinase I
casein kinase I homolog cki1
casein kinase I, alpha isoform
casein kinase I, delta isoform
casein kinase I, epsilon isoform
casein kinase I, gamma 1 isoform
casein kinase I, gamma 2 isoform
casein kinase I, gamma 3 isoform
casein kinase II
casein kinase2
CDPK
cell division cycle 7-related protein kinase
checkpoint serine/threonine-protein kinase bub1
Chk1
CK-2
CK1
CK1delta
CK1gamma
CK2
Cki3
CKII
CPK
CPK21
DAP-kinase
DNA damage-responsive serine/threonine-protein kinase
DNA-PKcs
DR2518
Env7
ESTK
eukaryotic type serine/threonine protein kinase
eukaryotic-like serine/threonine kinase
glycogen synthase kinase 3
glycogen synthase kinase-3
glycogen synthase kinase-3alphabeta
glycogen synthase kinase-3beta
GSK-3
GSK-3beta
GSK3
HIPK2
homeodomain-interacting protein kinase 2
KIAA0369
KpnK
LIM domain kinase 1
LKB1
MAP kinase-activated protein kinase 2
mBub1b
meiosis-specific serine/threonine-protein kinase MEK1
membrane-associated protein-serine/threonine kinase
microtubule-associated serine/threonine kinase family
-
mitotic checkpoint serine/threonine-protein kinase BUB1
mitotic checkpoint serine/threonine-protein kinase BUB1 beta
MPK38
mycobacterial serine/threonine protein kinase
myotonin-protein kinase
NDR1
negative regulator of sexual conjugation and meiosis
-
ovarian-specific serine/threonine-protein kinase Lok
-
p-AKT
P21-activated kinase-1
p53-related protein kinase
p70 S6 kinase
p90 ribosomal S6 kinase
Pak1
PDK1
peripheral plasma membrane protein CASK
phosphatidylinositol 3-kinase
phosphoinositide 3-kinase
phosphoinositide-dependent protein kinase-2
Phototropin
PI3K
PIM1
Pim1 serine/threonine kinase
PKA
PkaE
PkaG
PKB
Pkb1
Pkb2
Pkb3
Pkb4
Pkb5
Pkb6
PKN
PknA
PknB
PknE
PknH
PknI
PknL
pprA
protein kinase A
protein kinase CK2
protein kinase KIN1
protein kinase Sgk
RAC-alpha serine/threonine kinase
RAC-beta serine/threonine protein kinase
RAC-gamma serine/threonine protein kinase
ribosomal protein S6 kinase
ribosomal protein S6 kinase alpha 1
ribosomal protein S6 kinase alpha 2
ribosomal protein S6 kinase beta 2
ribosomal protein S6 kinase II alpha
ribosomal S6 protein kinase
RMIL serine/threonine-protein kinase
RqkA
RSK
S6K1
S6K2
SCD10.09
Ser/Thr kinase
Ser/Thr protein kinase
serine-threonine kinase
serine/
serine/threonine kinase
serine/threonine kinase 17A
serine/threonine protein kinase
serine/threonine protein kinase 16
serine/threonine protein kinase 31
serine/threonine-protein kinase
serine/threonine-protein kinase 1
serine/threonine-protein kinase 11
serine/threonine-protein kinase AtPK1/AtPK6
-
serine/threonine-protein kinase Chk1
serine/threonine-protein kinase Chk2
serine/threonine-protein kinase CLA4
serine/threonine-protein kinase DCAMKL1
serine/threonine-protein kinase fused
-
serine/threonine-protein kinase IRE1 precursor
-
serine/threonine-protein kinase KIN3
-
serine/threonine-protein kinase Kist
serine/threonine-protein kinase PAK 1
serine/threonine-protein kinase PAK 2
serine/threonine-protein kinase PAK 3
serine/threonine-protein kinase Sgk
serine/threonine-protein kinase transforming protein mil
-
serine/threonine-protein kinase transforming protein Rmil
serine/threonine-protein kinase ULK1
SSO0433
SsoPK3
SsoPK5
SSTK
STE20/SPS1-related proline-alanine rich protein kinase
STK11
StkP
StpK
testis-specific serine/threonine kinase
threonine Pim kinase
TSSK1
Tssk4
TSSK5
Yck1p
Yck2p
additional information
casein kinase 2
-
-
CK2
-
-
DNA damage-responsive serine/threonine-protein kinase
UniProt
meiosis-specific serine/threonine-protein kinase MEK1
-
mitotic checkpoint serine/threonine-protein kinase BUB1
-
mitotic checkpoint serine/threonine-protein kinase BUB1 beta
-
myotonin-protein kinase
-
RAC-alpha serine/threonine kinase
-
RAC-gamma serine/threonine protein kinase
-
serine/threonine protein kinase
-
serine/threonine protein kinase
-
serine/threonine protein kinase
-
serine/threonine-protein kinase 1
-
serine/threonine-protein kinase 1
-
serine/threonine-protein kinase Chk1
-
serine/threonine-protein kinase Sgk
-
serine/threonine-protein kinase transforming protein Rmil
-
serine/threonine-protein kinase transforming protein Rmil
-
STE20/SPS1-related proline-alanine rich protein kinase
-
additional information
the enzyme belongs to the Rio family, Rio2 subfamily, of serine/threonine protein kinases
additional information
-
PKN belongs to the AGC subfamily of protein kinases
additional information
-
PKN belongs to the AGC subfamily of protein kinases
additional information
-
the enzyme belongs to the CKI family of serine/threonine protein kinases
additional information
-
PKD2 belongs to the PKD family of serine/threonine kinases
additional information
-
the enzyme belongs to the CKI family of serine/threonine protein kinases
additional information
TSSK5 is a member of the testis-specific serine/threonine kinase family
additional information
-
the enzyme belongs to the p90RSK, RSK, family of proteins
additional information
-
the enzyme TSSK3 belongs to the family of testis-specific serine-threonine kinases
additional information
-
PKN belongs to the AGC subfamily of protein kinases
additional information
-
the enzyme belongs to the CKI family of serine/threonine protein kinases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
-
-
-
-
ATP + a protein = ADP + a phosphoprotein
active site and activation loop structure and conformation, Lys233, Cys250, Asp368, and Thr386 are important for activity, the substrate binding groove determines the substrate specificity via residues Val318 and Ala448
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
catalytic mechanism and regulation of PKN
-
ATP + a protein = ADP + a phosphoprotein
glycogen synthase kinase-3beta also performs the tau-protein kinase reaction, EC 2.7.11.26
-
ATP + a protein = ADP + a phosphoprotein
Lys45 is important for PknH catalytic and autophosphorylation activity
-
ATP + a protein = ADP + a phosphoprotein
the enzyme shows Mn2+-dependent serine/threonine kinase and Ca2+-dependent histidine kinase, EC 2.7.13.3, activities
ATP + a protein = ADP + a phosphoprotein
the CK1 family shows a strong preference both in vitro and in vivo for primed, pre-phosphorylated substrates. CK1 family members prefer acidic substrates in vitro, most notably those with acidic residues in the N-3 position, e.g. D-X-X-S/T. The most effective motif contains a phospho-residue in the N-3 position, such as pS/pT-X-X-S/T, where pS/pT refers to a phospho-serine or phospho-threonine, X refers to any amino acid, and the underlined residues refer to the target site
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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CAS REGISTRY NUMBER
COMMENTARY
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP + casein
AMP + phosphorylated casein
-
phosphorylated on threonine residues
-
?
ATP +
ADP + phosphorylated DNA-recombinase RecA
-
phosphorylation at serine 2
-
?
ATP + 3-hydroxy-3-methylglutaryl-CoA reductase
ADP + phosphorylated 3-hydroxy-3-methylglutaryl-CoA reductase
-
-
?
ATP + AaSTPK
ADP + phosphorylated-AaSTPK
enzyme is autophosphorylated on a threonine residue
-
?
ATP + ABCA1 NBD1-R1 protein
ADP + phosphorylated ABCA1 NBD1-R1 protein
-
substrate protein is a recombinant construct of wild-type and mutant nucleotide binding domain 1 and cytoplasmic domain R1 of ATP-binding cassette transporter protein A1 expressed in Escherichia coli, phosphorylation at T1242, T1243, and S1255
-
?
ATP + ABCA1 NBD2-R2 protein
ADP + phosphorylated ABCA1 NBD2-R2 protein
-
substrate protein is a recombinant construct of wild-type and mutant nucleotide binding domain 2 and cytoplasmic domain R2 of ATP-binding cassette transporter protein A1 expressed in Escherichia coli
-
?
ATP + Ac-LRRASLG
ADP + Ac-LRRApSLG
-
an efficient fluorescence assay for serine/threonine kinases using a peptide array is developed. Assay is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated beta-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the phosphorylated peptide. Novel fluorescent reagents are designed for this purpose and synthesized. As a model peptide, a well-known PKA consensus sequence Kemptide 49 is chosen
-
?
ATP + Ac-RPRAASF
ADP + Ac-Ac-RPRAApSF
an efficient fluorescence assay for serine/threonine kinases using a peptide array is developed. Assay is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated beta-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the phosphorylated peptide. Novel fluorescent reagents are designed for this purpose and synthesized
-
?
ATP + adenomatous polyposis coli protein
ADP + phosphorylated adenomatous polyposis coli protein
ATP + AfsR
ADP + ?
phosphorylation at both Ser and Thr residues
-
?
ATP + AIB1 protein
ADP + phosphorylated AIB1 protein
-
phosphorylation at S601 by isozyme CK1delta
-
?
ATP + Akt (MBP)
ADP + phosphorylated-Akt
purified Pak1 can directly phosphorylate Akt only at Ser473, suggesting that Pak1 may be a relevant PDK2 responsible for AKT Ser473 phosphorylation in cardiomyocytes
-
?
ATP + AMP-activated protein kinase
ADP + phosphorylated AMP-activated protein kinase
-
-
-
?
ATP + AMP-activated protein kinase alpha1
ADP + phospho-AMP-activated protein kinase
phosphorylation of Ser485 at subunit alpha1 and of Ser491 at subunit alpha2
-
?
ATP + apoptosis signal-regulating kinase 1
ADP + phosphorylated-apoptosis signal-regulating kinase 1
MPK28 stimulates APK1 activity by Thr838 phosphorylation
-
?
ATP + ataxin-3 protein
ADP + phosphorylated ataxin-3 protein
-
phosphorylation at serines 340 and 352
-
?
ATP + BAF170
ADP + phosphorylated BAF170
-
BAF170 is a hSWI/SNF protein that contains one Akt consensus site
-
?
ATP + beta-hydroxy beta-methylglutaryl-coenzyme A reductase
ADP + phosphorylated beta-hydroxy beta-methylglutaryl-coenzyme A reductase
phosphorylation and inactivation
-
?
ATP + calmodulin
ADP + phosphorylated calmodulin
-
phosphorylation by isozyme CK1alpha
-
?
ATP + CARMA1 protein
ADP + phosphorylated CARMA1
-
i.e. CARD11 protein, phosphorylation at S608 by isozyme CK1alpha
-
?
ATP + Cdc42Hs
ADP + ?
PAK4 interacts only with the activated form of Cdc42Hs through its GTPase-binding domain
-
?
ATP + Cdk2 interacting protein
ADP + phosphorylated Cdk2 interacting protein
-
-
-
?
ATP + cell division cycle 7
ADP + phosphorylated cell division cycle 7
-
-
-
?
ATP + claudin-4
ADP + phosphorylated claudin-4
-
recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli
-
?
ATP + CPS.A subunit
ADP + phosphorylated-CPS.A subunit
AaSTPK also phosphorylates one threonine residue on the carbamoyl phosphate synthetase, CPS.A subunit
-
?
ATP + Crem protein
ADP + phosphorylated Crem protein
the enzyme phosphorylates Crem protein at Ser117
-
?
ATP + Dnmt1 protein
ADP + phosphorylated Dnmt1 protein
-
phosphorylation at S147 by isozymes CK1delta and CK1epsilon
-
?
ATP + eukaryotic protein synthesis initiation factor
?
-
i.e. eIF-4E, 1 mol phosphate per mol eIF-4E
-
?
ATP + FACT
ADP + phospho-FACT
-
the FACT complex can be phosphorylated in vitro in reactions containing recombinant CKII
-
?
ATP + FADD protein
ADP + phosphorylated FADD protein
-
phosphorylation at S194 by isozyme CK1alpha
-
?
ATP + glycogen synthase kinase-3 (GSK-3) fusion protein
ADP + phosphorylated- glycogen synthase kinase-3 fusion protein
-
-
?
ATP + granulocyte macrophage colony stimulating factor receptor
ADP + phospho-granulocyte macrophage colony stimulating factor receptor
ATP + GroES protein
ADP + phosphorylated GroES protein
-
-
?
ATP + histine H2A
ADP + histidine gammaH2A
-
phosphorylation on serine 129 in vivo
Glc7 dephosphorylates histidine gammaH2A in vitro
?
ATP + histone f1
?
-
lysine-rich histone, phosphorylated at a specific serine residue
-
?
ATP + histone fraction II
?
-
slightly lysine-rich, from trout testes
-
?
ATP + histone H3
?
Ark1 phosphorylates Ser10 of histone H3 in vivo
-
?
ATP + histone H3
ADP + phosphorylated histone
Ark1 phosphorylates Ser10 of histone H3 in vivo
-
?
ATP + ICAP-1alpha protein
ADP + phosphorylated ICAP-1alpha protein
-
phosphorylated at threonine 38
-
?
ATP + IFNAR1 protein
ADP + phosphorylated IFNAR1 protein
-
phosphorylation at S535 by isozyme CK1alpha
-
?
ATP + IgG2A heavy chain
ADP + phosphorylated IgG2A heavy chain
-
substrate protein from mouse
-
?
ATP + KKRAARASSNVFA
ADP + ?
-
phosphorylation of the peptide is undetectable at 25°C. Phosphorylation takes place at measurable rates if the assay temperature is raised to 65°C or if poly(Glu4:Tyr) is included in the assay mixture
-
?
ATP + KKRNRRLSVA
ADP + KKRNRRL-phosphoserine-VA
-
peptide substrate of NDR1
-
?
ATP + KLP10A protein
ADP + phosphorylated KLP10A protein
-
phosphorylation at S573 by isozyme CK1alpha
-
?
ATP + late gene expression factor LEF-8
ADP + phosphorylated-late gene expression factor LEF-8
-
-
?
ATP + light-harvesting complex II
ADP + phospho-light-harvesting complex II
-
-
-
?
ATP + light-harvesting complex II
ADP + phosphorylated light-harvesting complex II
Chlamydomonas sp.
-
-
-
?
ATP + lysozyme
ADP + phopshorylated lysozyme
-
reduced carboxyamidomethylated and maleylated lysozyme
-
?
ATP + M3-muscarnic receptor
ADP + phosphorylated M3-muscarnic receptor
-
-
-
?
ATP + maize eukaryotic translation initiation factor 5A
ADP + phosphorylated maize eukaryotic translation initiation factor 5A
ATP + maltose-binding protein
ADP + phosphorylated maltose-binding protein
-
-
-
?
ATP + MCM2
ADP + ?
wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro
-
?
ATP + MCM3
ADP + ?
wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro
-
?
ATP + myelic basic protein
ADP + myelic basic phosphoprotein
-
phosphorylation of serine and threonine residues by PknB
-
?
ATP + myelin basic protein (MBP)
ADP + phosphorylated-myelin basic protein
-
-
?
ATP + myelin basic protein kinase
ADP + phosphorylated myelin basic protein kinase
-
-
?
ATP + NKCC1-(1-260)
ADP + phosphorylated NKCC1-(1-260)
-
i.e. Na+-K+-2Cl- co-transporter-1-(1-260) construct, recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli
-
?
ATP + non-structural protein 2
ADP + phospho-non-structural protein 2
-
non-structural protein 2 from bluetongue virus (BTV)
-
?
ATP + non-structural protein 2
ADP + phosphorylated non-structural protein 2
-
-
-
?
ATP + OdhI
ADP + phosphorylated-OdhI
following phosphorylation by PknA, PknG can transphosphorylate its specific substrate 2-oxoglutarate dehydrogenase inhibitor protein (OdhI) in vitro
-
?
ATP + ORF62 protein
ADP + ORF62 phosphoprotein
-
protein substrate from Varicella-Zoster virus, no activity with a truncated ORF62 mutant
-
?
ATP + ORF63 protein
ADP + ORF63 phosphoprotein
-
protein substrate from Varicella-Zoster virus
-
?
ATP + OTUB1
ADP + phospho-OTUB1
-
OTUB1 is a tumor domain protease, phosphorylation at Ser16, in GSDSEGVN
-
?
ATP + OTUB1 protein
ADP + phosphorylated OTUB1 protein
-
phosphorylation at serine 16
-
?
ATP + OTUD5 protein
ADP + phosphorylated OTUD5 protein
-
phosphorylation at serine 177
-
?
ATP + oxidative stress response kinase 1 OSR1
ADP + phosphorylated oxidative stress response kinase 1 OSR1
-
recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli, phosphorylation at Ser325 and Thr185, the latter is required for activation of OSR1
-
?
ATP + PACSIN protein
ADP + phosphorylated PACSIN protein
-
phosphorylation at Ser358
-
?
ATP + Pah1 phosphatidate phosphatase
ADP + phosphorylated Pah1 phosphatidate phosphatase
-
more than 90% of its phosphorylation occurs on Thr-170, Ser-250, Ser-313, Ser-705, Ser-814, and Ser-818
-
?
ATP + peptide LRRKtide
ADP + phosphorylated peptide LRRKtide
-
high activity towards the peptidic substrate LRRKtide
-
?
ATP + PHLPP1 protein
ADP + phosphorylated PHLPP1 protein
-
phosphorylation at T851 by isozyme CK1alpha
-
?
ATP + phosphoglycogen synthase peptide 2
ADP + phosphorylated phosphoglycogen synthase peptide 2
ATP + PKD2 protein
ADP + phosphorylated PKD2 protein
-
phosphorylation at S244 by isozymes CK1alpha, CK1delta, and CK1epsilon
-
?
ATP + PknG
ADP + phosphorylated-PknG
activation of PknG is part of a phosphorylation cascade mechanism that relies on PknA activity
-
?
ATP + plant ribosomal proteins
ADP + phosphorylated plant ribosomal proteins
two plant ribosomal proteins of 14000 Da and 16000 Da can be phosphorylated by the Atpk1 protein kinase
-
?
ATP + protein kinase Czeta
ADP + phosphorylated protein kinase Czeta
phosphorylation and activation of protein kinase Czeta
-
?
ATP + protein p21
ADP + phosphoprotein p21
-
threonine 57, threonine 145, serine 98, serine 130, and serine 146 are phosphorylated by JNK, JNK mediates stabilization of protein p21 via the phosphorylation of threonine 57
-
?
ATP + Rec8 protein
ADP + phosphorylated Rec8 protein
-
phosphorylation at S412 by isozyme CK1delta and CK1epsilon, and at S400, S402, S404, S410, T414, and S416
-
?
ATP + reduced carboxyamidomethylated and maleylated lysozyme
ADP + phosphorylated reduced carboxyamidomethylated and maleylated lysozyme
-
phosphorylated on threonine residues
-
?
ATP + reduced carboxymethylated lysozyme
ADP + phopshorylated reduced carboxymethylated lysozyme
ATP + ribosomal protein S6
ADP + phosphorylated ribosomal protein S6
specifically phosphorylated by S6K
-
?
ATP + RNP29
ADP + phospho-RNP29
recombinant Strep-tagged substrate expressed from Escherichia coli, phosphorylation of the canonical pCKII motif ESEGE in the model substrate, identification of the RNP29 phosphorylation site by peptide mapping and mass spectrometry
-
?
ATP + RRRDDDSDDD
ADP + RRRDDDphosphoSDDD
-
a CK2-specific synthetic peptide substrate
-
?
ATP + RRRLSSLRA
ADP + RRRLSpSLRA
-
enzyme activities are determined by measuring initial velocities for His6-S6K1aII(DELTA AID)-T389E catalyzed phosphorylation of a commercially available S6K/RSK model peptide substrate 1 (S6K1-Tide, RRRLSSLRA)
-
?
ATP + RRSSSY
ADP + RRSS-phosphoS-Y
-
peptide substrate of TSSK3 consisting of the consensus sequence
-
?
ATP + RSRSRSRSRSRSPPPVSK
ADP + phosphorylated RSRSRSRSRSRSPPPVSK
-
SC35-derived peptide 180-197, recombinant GSK-3beta
-
?
ATP + S6 protein of the 40s ribosomal subunit
ADP + phosphorylated S6 protein of the 40s ribosomal subunit
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