Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-glutamate | isozyme AAT1 and AAT2, product inhibition | Lupinus albus | |
oxaloacetate | isozyme AAT1 and AAT2, product inhibition | Lupinus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Lupinus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52200 | - |
2 * 52200, isozyme AAT1, SDS-PAGE | Lupinus albus |
54800 | - |
2 * 54800, isozyme AAT2, SDS-PAGE | Lupinus albus |
88000 | - |
isozyme AAT1, gel filtration | Lupinus albus |
92000 | - |
isozyme AAT2, gel filtration | Lupinus albus |
112000 | - |
isozyme AAT1, native PAGE | Lupinus albus |
136000 | - |
isozyme AAT2, native PAGE | Lupinus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Lupinus albus | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus albus | - |
cv. Estoril | - |
Lupinus albus | - |
2 isozymes: AAT1 and AAT2 | - |
Purification (Comment) | Organism |
---|---|
isozymes AAT1 and AAT2 | Lupinus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | isozymes AAT1 and AAT2 | Lupinus albus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Lupinus albus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Lupinus albus | oxaloacetate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54800, isozyme AAT2, SDS-PAGE | Lupinus albus |
dimer | 2 * 52200, isozyme AAT1, SDS-PAGE | Lupinus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Lupinus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
isozyme AAT1 t1/2: 3.6 min, isozyme AAT2 t1/2: 41.2 min | Lupinus albus |
65 | - |
inactivation, isozymes AAT 1 and 2 | Lupinus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | isozyme AAT1 | Lupinus albus |
8.5 | 9.5 | isozyme AAT2 | Lupinus albus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
oxaloacetate | isozyme AAT1, competitive versus 2-oxoglutarate | Lupinus albus | |
0.024 | - |
oxaloacetate | isozyme AAT2, noncompetitive versus L-aspartate | Lupinus albus | |
0.026 | - |
oxaloacetate | isozyme AAT2, competitive versus 2-oxoglutarate | Lupinus albus | |
0.03 | - |
oxaloacetate | isozyme AAT1, noncompetitive versus L-aspartate | Lupinus albus | |
10.5 | - |
L-glutamate | isozyme AAT1, competitive versus L-aspartate | Lupinus albus | |
11.1 | - |
L-glutamate | isozyme AAT1, noncompetitive versus 2-oxoglutarate | Lupinus albus | |
16 | - |
L-glutamate | isozyme AAT2, noncompetitive versus 2-oxoglutarate | Lupinus albus | |
30 | - |
L-glutamate | isozyme AAT2, competitive versus L-aspartate | Lupinus albus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lupinus albus | isozyme AAT1 | - |
5.54 |
Lupinus albus | isozyme AAT2 | - |
5.79 |