BRENDA - Enzyme Database
show all sequences of 2.4.2.42

Purification and substrate specificity of UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase involved in the biosynthesis of the Xylalpha1-3Xylalpha1-3Glcbeta1-O-Ser on epidermal growth factor-like domains

Ishimizu, T.; Sano, K.; Uchida, T.; Teshima, H.; Omichi, K.; Hojo, H.; Nakahara, Y.; Hase, S.; J. Biochem. 141, 593-600 (2007)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
20 mM, 85% loss of activity
Bos taurus
Cu2+
20 mM, complete loss of activity
Bos taurus
EDTA
-
Bos taurus
Fe2+
20 mM, complete loss of activity
Bos taurus
Ni2+
20 mM, 90% loss of activity
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
pH 7.2, 37°C
Bos taurus
4.2
-
2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
pH 7.2, 37°C
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
20 mM, 2fold increase in activity
Bos taurus
additional information
20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
Bos taurus
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.075
-
-
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with UDP-D-glucose
698648
Bos taurus
?
-
-
-
-
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
-
698648
Bos taurus
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
-
-
-
?
UDP-D-xylose + D-glucose
-
698648
Bos taurus
UDP + Xylalpha(1-3)Glc
-
-
-
?
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
around
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
20 mM, 85% loss of activity
Bos taurus
Cu2+
20 mM, complete loss of activity
Bos taurus
EDTA
-
Bos taurus
Fe2+
20 mM, complete loss of activity
Bos taurus
Ni2+
20 mM, 90% loss of activity
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
pH 7.2, 37°C
Bos taurus
4.2
-
2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
pH 7.2, 37°C
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
20 mM, 2fold increase in activity
Bos taurus
additional information
20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
Bos taurus
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.075
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with UDP-D-glucose
698648
Bos taurus
?
-
-
-
-
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
-
698648
Bos taurus
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
-
-
-
?
UDP-D-xylose + D-glucose
-
698648
Bos taurus
UDP + Xylalpha(1-3)Glc
-
-
-
?
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide
698648
Bos taurus
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
around
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity
Bos taurus
Other publictions for EC 2.4.2.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698648
Ishimizu
Purification and substrate spe ...
Bos taurus
J. Biochem.
141
593-600
2007
-
-
-
-
-
-
5
2
1
2
-
1
-
2
-
-
1
-
-
1
1
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
1
2
-
1
-
-
-
1
-
1
1
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
697698
Omichi
Presence of UDP-D-xylose: beta ...
Homo sapiens
Eur. J. Biochem.
245
143-146
1997
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-