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Literature summary for 2.4.1.329 extracted from

  • Franceus, J.; Capra, N.; Desmet, T.; Thunnissen, A.
    Structural comparison of a promiscuous and a highly specific sucrose 6F-phosphate phosphorylase (2019), Int. J. Mol. Sci., 20, 3906 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.329

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ilumatobacter coccineus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure at 2.05 A resolution, comparison with structure of the Thermoanaerobacterium thermosaccharolyticum enzyme. The residues participating in hydrogen bonds with the phosphate are Arg152, Lys364, Lys434, and Tyr377 Ilumatobacter coccineus

Protein Variants

Protein Variants Comment Organism
K364S mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
K434A strong decrease in kcat value Ilumatobacter coccineus
K434R mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
R152A mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
Y377H mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
2.8
-
 D-fructose 6-phosphate mutant Y377H, pH 6.5, 35°C Ilumatobacter coccineus
3 3.2 D-fructose 6-phosphate mutant K434A, pH 6.5, 35°C Ilumatobacter coccineus
3.3
-
 D-fructose 6-phosphate mutant K152A, pH 6.5, 35°C Ilumatobacter coccineus
6.3
-
 D-fructose 6-phosphate mutant K364S, pH 6.5, 35°C Ilumatobacter coccineus
7.8
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
10.6
-
 D-fructose 6-phosphate mutant K434R, pH 6.5, 35°C Ilumatobacter coccineus
11.3
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
18.8
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus

Organism

Organism UniProt Comment Textmining
Ilumatobacter coccineus A0A6C7EEG6
-
-
Ilumatobacter coccineus YM16-304 A0A6C7EEG6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
 pH 6.5, 35°C Ilumatobacter coccineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
-
 Ilumatobacter coccineus sucrose 6F-phosphate + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
-
 Ilumatobacter coccineus YM16-304 sucrose 6F-phosphate + phosphate
-
 r
additional information enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate Ilumatobacter coccineus ?
-
-
additional information enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate Ilumatobacter coccineus YM16-304 ?
-
-
sucrose 6F-phosphate + phosphate
-
 Ilumatobacter coccineus alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
-
 r
sucrose 6F-phosphate + phosphate
-
 Ilumatobacter coccineus YM16-304 alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 59900, SDS-PAGE, recombinant protein Ilumatobacter coccineus

Synonyms

Synonyms Comment Organism
YM304_32550
-
 Ilumatobacter coccineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Ilumatobacter coccineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.61
-
 D-fructose 6-phosphate mutant K364S, pH 6.5, 35°C Ilumatobacter coccineus
0.8
-
 D-fructose 6-phosphate mutant Y377H, pH 6.5, 35°C Ilumatobacter coccineus
1.42
-
 D-fructose 6-phosphate mutant K152A, pH 6.5, 35°C Ilumatobacter coccineus
3.5
-
 D-fructose 6-phosphate mutant K434A, pH 6.5, 35°C Ilumatobacter coccineus
6.84
-
 D-fructose 6-phosphate mutant K434R, pH 6.5, 35°C Ilumatobacter coccineus
40
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
45
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
110
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
126
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 phosphorolysis of sucrose 6F-phosphate Ilumatobacter coccineus
6.5
-
 synthesis of sucrose 6F-phosphate Ilumatobacter coccineus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 more than 50% of maximum activity, synthesis of sucrose 6F-phosphate Ilumatobacter coccineus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
11.2
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
14.1
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
20
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus