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Information on EC 2.4.1.329 - sucrose 6F-phosphate phosphorylase
for references in articles please use BRENDA:EC2.4.1.329
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.329 sucrose 6F-phosphate phosphorylase
IUBMB Comments
The enzyme, isolated from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum, catalyses the reversible phosphorolysis of sucrose 6F-phosphate. It also acts on sucrose with lower activity.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
sucrose 6'-phosphate phosphorylase, sucrose 6f-phosphate phosphorylase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
SPP
YM304_32550
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose 6F-phosphate + phosphate = alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
sucrose 6F-phosphate:phosphate 1-alpha-D-glucosyltransferase
The enzyme, isolated from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum, catalyses the reversible phosphorolysis of sucrose 6F-phosphate. It also acts on sucrose with lower activity.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
sucrose + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose
Substrates: 51% compared to the activity with sucrose 6F-phosphate
Products: -
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r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
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r
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
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r
alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
sucrose 6F-phosphate + phosphate
Substrates: -
Products: -
Products: -
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: strictly specific to sucrose 6F-phosphate
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: strictly specific to sucrose 6F-phosphate
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
Substrates: strictly specific to sucrose 6F-phosphate
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: clear preference for sucrose 6F-phosphate
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: clear preference for sucrose 6F-phosphate
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
r
additional information
?
-
Substrates: enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate
Products: -
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?
additional information
?
-
-
Substrates: enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate
Products: -
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?
additional information
?
-
Substrates: enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate
Products: -
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?
additional information
?
-
Substrates: enzyme displays selective activity on sucrose 6F-phosphate acting both in phosphorolysis releasing alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate, and in reverse phosphorolysis from alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate to sucrose 6F-phosphate. No substrates: sucrose, D-fructose, D-glucose, D-galalctose, D-mannose, D-N-acetylgalactosamine, D-xylose, maltotetraose, maltoheptaose
Products: -
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?
additional information
?
-
-
Substrates: no activity with trehalose 6-phosphate
Products: -
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?
additional information
?
-
Substrates: no activity with trehalose 6-phosphate
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?
additional information
?
-
-
Substrates: enzyme also acts on sucrose, reaction of EC 2.4.1.7, with lower activity. No substrate for phosphorolysis: trehalose 6-phosphate. No substrates for synthesis: D-psicose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, D-glucose, D-mannose, D-galactose, D-maltose, maltodextrin DE-15
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme also acts on sucrose, reaction of EC 2.4.1.7, with lower activity. No substrate for phosphorolysis: trehalose 6-phosphate. No substrates for synthesis: D-psicose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, D-glucose, D-mannose, D-galactose, D-maltose, maltodextrin DE-15
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme also acts on sucrose, reaction of EC 2.4.1.7, with lower activity. No substrate for phosphorolysis: trehalose 6-phosphate. No substrates for synthesis: D-psicose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, D-glucose, D-mannose, D-galactose, D-maltose, maltodextrin DE-15
Products: -
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
Substrates: -
Products: -
Products: -
?
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
Substrates: -
Products: -
Products: -
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
15.6
alpha-D-glucopyranose 1-phosphate
pH 6.0, 55°C
15.1
beta-D-fructofuranose 6-phosphate
pH 6.0, 55°C
1 - 3
sucrose 6F-phosphate
pH and temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
14.4
alpha-D-glucopyranose 1-phosphate
pH 6.0, 55°C
24.2
beta-D-fructofuranose 6-phosphate
pH 6.0, 55°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.9
alpha-D-glucopyranose 1-phosphate
pH 6.0, 55°C
0.4
beta-D-fructofuranose 6-phosphate
pH 6.0, 55°C
1.6
beta-D-fructofuranose 6-phosphate
pH 6.0, 55°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 8
more than 50% of maximum activity, synthesis of sucrose 6F-phosphate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (158 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
56000
x * 56000 SDS-PAGE, x * 56700, calculated
56700
x * 56000 SDS-PAGE, x * 56700, calculated
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
1 * 55000, SDS-PAGE, 1 * 55165, calculated from sequence
?
x * 56000 SDS-PAGE, x * 56700, calculated
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure at 2.05 A resolution, comparison with structure of the Thermoanaerobacterium thermosaccharolyticum enzyme. The residues participating in hydrogen bonds with the phosphate are Arg152, Lys364, Lys434, and Tyr377
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K364S
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
K434R
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
R152A
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
Y377H
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
K364S
-
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
-
K434R
-
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
-
R152A
-
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
-
Y377H
-
mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value
-
H344Y
decreased ratio of activity on phosphorylated fructose over fructose
K301A
displays similar activities as the wild type enzyme
R134A
decreased ratio of activity on phosphorylated fructose over fructose
R135A
displays similar activities as the wild type enzyme
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Verhaeghe, T.; Aerts, D.; Diricks, M.; Soetaert, W.; Desmet, T.
The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity
Appl. Microbiol. Biotechnol.
98
7027-7037
2014
Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum (D9TT09), Thermoanaerobacterium thermosaccharolyticum DSM 571 (D9TT09)
brenda
Franceus, J.; Capra, N.; Desmet, T.; Thunnissen, A.
Structural comparison of a promiscuous and a highly specific sucrose 6F-phosphate phosphorylase
Int. J. Mol. Sci.
20
3906
2019
Ilumatobacter coccineus (A0A6C7EEG6), Ilumatobacter coccineus, Ilumatobacter coccineus YM16-304 (A0A6C7EEG6)
brenda
Tauzin, A.; Bruel, L.; Laville, E.; Nicoletti, C.; Navarro, D.; Henrissat, B.; Perrier, J.; Potocki-Veronese, G.; Giardina, T.; Lafond, M.
Sucrose 6F-phosphate phosphorylase A novel insight in the human gut microbiome
Microb. Genom.
5
1-14
2019
Mediterraneibacter gnavus (G4T4R6)
brenda
Franceus, J.; Desmet, T.
Sucrose phosphorylase and related enzymes in glycoside hydrolase family 13 Discovery, application and engineering
Int. J. Mol. Sci.
21
2526
2020
Thermoanaerobacterium thermosaccharolyticum (D9TT09), Ilumatobacter coccineus (A0A6C7EEG6), Mediterraneibacter gnavus, Thermoanaerobacterium thermosaccharolyticum DSM 571 (D9TT09), Ilumatobacter coccineus NBRC 103263 (A0A6C7EEG6)
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.329)
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