Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Catharanthus roseus | |
5.38 | - |
7-deoxyloganetate | pH 8.0, 30°C | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 7-deoxyloganetate | Catharanthus roseus | the enzyme is involved in loganin and secologanin biosynthesis | UDP + 7-deoxyloganate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | U3U992 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, purified by nickel nitrilotriacetic acid affinity chromatography | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | preferentially expressed in internal phloem associated parenchyma cells | Catharanthus roseus | - |
phloem | preferentially expressed in internal phloem associated parenchyma cells | Catharanthus roseus | - |
plant parenchyma | preferentially expressed in internal phloem associated parenchyma cells | Catharanthus roseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 7-deoxyloganetate | the enzyme is involved in loganin and secologanin biosynthesis | Catharanthus roseus | UDP + 7-deoxyloganate | - |
? | |
UDP-alpha-D-glucose + 7-deoxyloganetate | does not react with 7-deoxyloganetin | Catharanthus roseus | UDP + 7-deoxyloganate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UGT8 | - |
Catharanthus roseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Catharanthus roseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Catharanthus roseus | |
0.325 | - |
7-deoxyloganetate | pH 8.0, 30°C | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in loganin and secologanin biosynthesis | Catharanthus roseus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
7-deoxyloganetate | pH 8.0, 30°C | Catharanthus roseus | |
1.5 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Catharanthus roseus |