Protein Variants | Comment | Organism |
---|---|---|
S18A/H127A | OGlcNAcylation on the mutant enzyme sharply declines | Homo sapiens |
S52A | O-GlcNAcylation on the mutant enzyme increases | Homo sapiens |
S56A | O-GlcNAcylation on the mutant enzyme increases | Homo sapiens |
T12A | O-GlcNAcylation on the mutant enzyme declines | Homo sapiens |
T12A/H127A | OGlcNAcylation on the mutant enzyme sharply declines | Homo sapiens |
T12G | sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase | Homo sapiens |
T12L | sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase | Homo sapiens |
T12Y | sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1757 | - |
UDP-N-acetyl-alpha-D-glucosamine | 37°C, pH not specified in the publication, enzyme form without O-GlcNAcylation | Homo sapiens | |
0.1805 | - |
UDP-N-acetyl-alpha-D-glucosamine | 37°C, pH not specified in the publication, O-GlcNAcylated enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O15294 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | O-GlcNAcylation of Ser389 in nucleocytoplasmic O-GlcNAc transferase affects its nuclear translocation in HeLa cells. Six O-GlcNAc sites are identified in the tetratricopeptide repeat domain in short-form of O-GlcNAc transferase, with Thr12 and Ser56 being two key sites. Thr12 is a dominant O-GlcNAcylation site. The modification of Ser56 plays a role in regulating short-form of O-GlcNAc transferase. O-GlcNAcylation does not affect activity of O-GlcNAc transferase but alters its substrate selectivity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + FITC-YAVVPVSK peptide | - |
Homo sapiens | UDP + ? | - |
? |