Inhibitors | Comment | Organism | Structure |
---|---|---|---|
xyloglucan-derived oligosaccharide | - |
Tropaeolum majus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
deglycosylated major enzyme form XET(6.3), SDS-PAGE | Tropaeolum majus |
29000 | - |
glycosylated major enzyme form XET(6.3), SDS-PAGE | Tropaeolum majus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tropaeolum majus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Tropaeolum majus |
Purification (Comment) | Organism |
---|---|
polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration | Tropaeolum majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Tropaeolum majus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + acceptor beta-(1,4)-xylooligosaccharide | the transglycosylating activity is 0.31% of that determined with xyloglucan oligosaccharide as acceptor | Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor beta-(1,6)-D-gluco-oligosaccharide | the transglycosylating activity is 0.69% of that determined with xyloglucan oligosaccharide as acceptor | Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor cellooligosaccharide | the transglycosylating activity is 4.6% of that determined with xyloglucan oligosaccharide as acceptor | Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor laminarioligosaccharide | the transglycosylating activity is 0.23% of that determined with xyloglucan oligosaccharide as acceptor | Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor mixed-linkage beta-(1,3 1,4)-gluco-oligosaccharide | the transglycosylating activity is 2.06% of that determined with xyloglucan oligosaccharide as acceptor | Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor pustulooligosaccharide | - |
Tropaeolum majus | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Tropaeolum majus | ? | - |
? | |
additional information | the enzyme exhibits broad substrate specificity by transferring xyloglucan or hydroxyethylcellulose fragments not only to oligoxyloglucosides and cellooligosaccharides but also to oligosaccharides derived from beta-(1,4)-D-glucuronoxylan, beta-(1,6)-D-glucan, mixed-linkage beta-(1,3 1,4)-D-glucan and at a relatively low rate also to beta-(1,3)-gluco-oligosaccharides | Tropaeolum majus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XET(6.3) | major enzyme form, XET(6.3) is classified into group II of the XTH phylogeny of glycoside hydrolase family GH16 | Tropaeolum majus |
XTH | - |
Tropaeolum majus |
xyloglucan endotransglycosylase | - |
Tropaeolum majus |
xyloglucan endotransglycosylase/hydrolase | - |
Tropaeolum majus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Tropaeolum majus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 50 | the activity of XETs from nasturtium seeds is stable at 40°C during 2 h (at 38°C during 8 h), but it is completely lost at 50°C | Tropaeolum majus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | - |
Tropaeolum majus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tropaeolum majus | major enzyme form XET(6.3), isoelectric focusing | - |
6.3 |