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Information on EC 2.4.1.207 - xyloglucan:xyloglucosyl transferase
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EC Tree
2.4.1.207 xyloglucan:xyloglucosyl transferaseIUBMB Comments
Does not use cello-oligosaccharides as either donor or acceptor.
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Word Map
- 2.4.1.207
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seventh
- children
- women
- nerve
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postoperative
- pain
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school
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student
- artery
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eighth
- sign
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resect
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adolescent
- bilateral
- neck
- cranial
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tomography
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preoperative
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social
- pregnancy
- girl
- hypertension
- abdominal
- child
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discharged
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services
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depart
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posterior
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january
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ninth
- chest
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session
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admitted
- thoracic
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demographic
-
physician
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admission
- vertebra
-
radiograph
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skill
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radiological
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youth
- headache
- sinus
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rib
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peer
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policy
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committee
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satisfaction
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cavernous
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
2
=
+
2
=
+
Synonyms
sixth, xyloglucan endotransglycosylase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase, xyloglucan endotransglycosylase/hydrolase, xth31, xyloglucan endo-transglycosylase, atxth21, xth33, xth19, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AtXTH18
AtXTH19
AtXTH20
DcXTH1
DcXTH2
DcXTH3
DcXTH4
endo-xyloglucan transferase
-
-
-
-
endoxyloglucan transferase
EXGT
EXT
-
-
-
-
GenBank L46792-derived protein GI 950299
-
-
-
-
GenBank X93173-derived protein GI 1890573
-
-
-
-
GenBank X93174-derived protein GI 1890575
-
-
-
-
Genbank X93175-derived protein GI 1890577
-
-
-
-
GenBank Z97335-derived protein GI
-
-
-
-
GhXTH1
GhXTH2
mixed-linkage beta-glucan:xyloglucan endotransglucosylase
MLG:xyloglucan endotransglucosylase
MXE
NXET
-
-
-
-
PttXET16A
SlXTH5
XET
XET(6.3)
-
major enzyme form, XET(6.3) is classified into group II of the XTH phylogeny of glycoside hydrolase family GH16
XET16A
XET5
XTH
XTH/XET
XTH1
XTH10
XTH11
XTH12
XTH14
XTH18
XTH19
XTH2
XTH20
XTH3
XTH30
XTH4
XTH5
XTH6
XTH7
XTH8
XTH9
xyloglucan endo-transglycosylase
xyloglucan endo-transglycosylase/hydrolase
xyloglucan endo-transglycosylase/xyloglucan endo-transglycosylase/hydrolase
-
xyloglucan endotransglucosylase
xyloglucan endotransglucosylase-hydrolase30
xyloglucan endotransglucosylase/hydrolase
xyloglucan endotransglycosylase
xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
-
-
-
-
xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
-
-
-
-
xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
-
-
-
-
xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
-
-
-
-
xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
-
-
-
-
xyloglucan endotransglycosylase 16A
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana)
-
-
-
-
xyloglucan endotransglycosylase/hydrolase
xyloglucan recombinase
-
-
-
-
xyloglucan X93174-derived protein GI 1890575
-
-
-
-
xyloglucan xyloglucosyl transferase
xyloglucan-specific endo-(1->4)-beta-D-glucanase
-
-
-
-
xyloglucan:xyloglucanotransferase
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB)
-
-
-
-
ZmXTH1
in addition to ZmXTH1 and Zm1005, six other XTH genes are identified in maize EST databases
additional information
endoxyloglucan transferase
-
-
-
-
endoxyloglucan transferase
-
endoxyloglucan transferase
-
EXGT
-
-
-
-
EXGT
-
XET
-
-
-
-
XTH
-
XTH1
-
shows both transglycosylase and hydrolase activities when assayed in vitro (depending on the substrates), however, in planta the enzyme acts only as a hydrolase to degrade storage xyloglucan
xyloglucan endotransglucosylase/hydrolase
-
xyloglucan endotransglucosylase/hydrolase
-
xyloglucan endotransglucosylase/hydrolase
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
-
xyloglucan endotransglucosylase/hydrolase
-
xyloglucan endotransglycosylase
-
-
-
-
xyloglucan endotransglycosylase
-
xyloglucan endotransglycosylase
-
additional information
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
additional information
-
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
Q5Z6H1
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
Q6ZAN9
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
additional information
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
sequential mechanism
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
reaction mechanism
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
reaction mechanism
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
ping-pong bi bi reaction mechanism
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
double-displacement reaction mechanism
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
determination of cleavage sites within the donor substrate of isozymes
-
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
enzyme possesss the catalytic sequence motif EIDFE conserved in the glycosyl hydrolase family 16
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
substrate binding and catalytic mechanism, active site structure, structure-function study
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
the enzyme contains a catalytic sequence motif conserved in the glycosyl hydrolase family 16
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
transglycosylation
transglycosylation
-
transglycosylation
-
-
idling reaction
-
transglycosylation
-
-
endotransglycosylation, a xyloglucan chain is cleaved and transferred to another acceptor chain
-
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SYSTEMATIC NAME
IUBMB Comments
xyloglucan:xyloglucan xyloglucanotransferase
Does not use cello-oligosaccharides as either donor or acceptor.
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CAS REGISTRY NUMBER
COMMENTARY
141588-40-1
-
172279-31-1
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced) /xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
202012-34-8
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana) /genBank Z97335-derived protein GI 2244769
203402-40-8
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5) /genBank X93173-derived protein GI 1890573 /xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
203402-41-9
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA) /genBank X93174-derived protein GI 1890575 /xyloglucan X93174-derived protein GI 1890575 /xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
203402-42-0
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB) /genbank X93175-derived protein GI 1890577 /xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
205513-08-2
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor) /genBank L46792-derived protein GI 950299 /Xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
donor barley (1,3,1,4)-beta-D-glucan + acceptor cellooligosaccharide
?
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor barley (1,3,1,4)-beta-D-glucan + acceptor xyloglucan oligosaccharide
?
-
0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor carboxymethylcellulose + acceptor xyloglucan oligosaccharide
?
-
0.4% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor Glc8-based xyloglucan oligosaccharide + acceptor sulforhodamine-labelled xyloglucan oligosaccharide
?
-
-
-
?
donor hydroxyethylcellulose + acceptor cellooligosaccharide
?
-
0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor hydroxyethylcellulose + acceptor xyloglucan oligosaccharide
?
-
44.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor locust bean gum + acceptor xyloglucan oligosaccharide
?
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor sulfuric acid-swollen cellulose + acceptor xyloglucan oligosaccharide
?
-
5% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor tamarind xyloglucan + acceptor cellooligosaccharide
?
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor tamarind xyloglucan + acceptor xyloglucan
?
-
isozymes XTH14 and XTH26 show very high preference for xyloglucan as donor substrate, yet do not work exclusively on xyloglucan, there is a clear preference for the octasaccharide XXLGol, whereas XLLGol is the second best (tested) substrate for isozyme XTH14, it seems a rather poor substrate for isozyme XTH26, which prefers XXFGol
-
-
?
donor xyloglucan + acceptor beta-(1,4)-xylooligosaccharide
?
-
the transglycosylating activity is 0.31% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor beta-(1,6)-D-gluco-oligosaccharide
?
-
the transglycosylating activity is 0.69% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor mixed-linkage beta-(1,3 1,4)-gluco-oligosaccharide
?
-
the transglycosylating activity is 2.06% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
donor xyloglucan + Xyl(1-6)Glc(1-4)Glc
?
-
trimer XG-ol, only isomer M55a shows a slight activity, tamarind xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XLXG
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-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XXLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXGXXXG-ol, dimer of XXXG-ol, most effective acceptor, pea xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA
?
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
-
-
-
?
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
-
-
-
?
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
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-
-
-
?
hydroxyethyl cellulose + reduced xyloglucan-derived heptasaccharide
?
3.4% activity compared to tamarind xyloglycan
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-
?
pea xyloglucan + reduced xyloglucan-derived heptasaccharide
?
-
-
-
?
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol + xyloglucan
?
-
acceptor substrate: rhodamin-conjugated reduced xyloglucan-derived nonasaccharide, donor substrate: tamarind-seed xyloglucan, fluorescence assay
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?
tamarind xyloglucan + alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)-D-Glc
?
i.e. XGO, the enzyme activity is assayed by the colorimetric method, overview
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-
?
tamarind xyloglucan + reduced xyloglucan-derived heptasaccharide
?
100% activity
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-
?
tamarind xyloglucan + XLLG-9-aminopyrene-1,4,6-trisulfonate
?
XET activity assay
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-
?
tamarind xyloglucan + XXXG
?
the enzyme performs transglucosylation
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-
?
Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) + Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) 8-aminonaphthalene-1,3,6-trisulfonic acid
Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) 8-aminonaphthalene-1,3,6-trisulfonic acid + Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)
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-
-
?
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
-
acceptor and donor: Glc8-based XXXGXXXG
products: XXXGXXXGXXXG + XXXG
?
xyloglucan + 4-mercaptobutanoyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
-
4-mercaptobutanoyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc is a worse acceptor than Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
-
-
?
xyloglucan + barley (1,3 1,4)-beta-D-glucan
?
-
the rate of covalent bond formation with barley (1,3 1,4)-beta-D-glucan is significant but slower compared with that on tamarind xyloglucan
-
-
?
xyloglucan + hydroxyethylcellulose
?
-
the rate of covalent bond formation with hyrdoxyethylcellulose is comparable with that on tamarind xyloglucan
-
-
?
xyloglucan + monostearyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
-
monostearyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc is a worse acceptor than Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
-
-
?
xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
xyloglucan + Xylalpha(1,6)Glc-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
-
-
-
-
?
xyloglucan + xyloglucan oligosaccharide
?
the recombinant FcXTH2 protein displays mainly XEH activity and basal XET activity. The Sulova method for XET activity is based in the ability of long xyloglucans (XGs) to bind iodine: the transglycosylation activity causes a reduction in size of XGs that loses the ability to bind the dye. Thus, the XET activity causes the reduction in molecular weight of XGs by transglycosylation of portions of the XG donor to low relative molecular mass (low-Mr) oligosaccharide acceptors whereby no net formation of reducing ends takes place. Thus, the XET activity is assayed by following changes in absorbance at 620 nm
-
-
?
xyloglucan oligosaccharides + xyloglucan
? + xyloglucan
-
xyloglucans with MW of about 600-1000 kDa
MW about 50 kDa
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Xyl(alpha1-6)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
donor xyloglucan + acceptor cellooligosaccharide
?
-
the transglycosylating activity is 4.6% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor laminarioligosaccharide
?
-
the transglycosylating activity is 0.23% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Adiantum capillus-veneri
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Aloe sp.
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Anthurium upalaense
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Apium sp.
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
acceptor and donor substrate specificities of the isoenzymes TCH4, Meri-5, EXGT and XTR9
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
donors: TCH4 protein is more active against tamarind xyloglucan than nasturtium xyloglucan as donor, pea stem xyloglucan, acceptors: XLLG-ol, XXFG-ol
-
-
?
donor xyloglucan + acceptor xyloglucan
?
isoenzyme XTR9 has a clear preference for non-fucosylated xyloglucan polymers as donor, but not isoenzymes TCH4, Meri-5 and EXGT
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
specific for xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
TCH4 protein specifically transglycosylates only xyloglucan, cleavage of both fucosylated and nonfucosylated xyloglucans as donor substrates, but fucosyl content of the substrates lowers reaction rate, preferred acceptor substrate is the nonreducing terminus of high-MW xyloglucan, xyloglucan derived oligosaccharides are also utilized
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Azolla sp.
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
pronounced preference for xyloglucan oligosaccharides with backbones of 4 over 3 over 2 Glc residues as acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
XET forms a stable donor xyloglucan-XET complex
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Chamaecyparis sp.
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Commelina nilagirica
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
NtXET-1 has a prefence for smaller xyloglucan molecules as acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
donor: pine or tobacco xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
minimum acceptor structure is Xyl2Glc3
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
galactosyl or fucosyl side chains are not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: high MW xyloglucan, xyloglucan-derived oligosaccharides with at least two alpha-D-xylose residues, reducing group is not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
XET is present in cell walls in form of a competent glycosyl-enzyme complex which decomposes by transglycosylation of its glycan moiety to added xyloglucan-oligosaccharide acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
minimum acceptor structure is Xyl2Glc3
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
galactosyl or fucosyl side chains are not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
tamarind seed xyloglucan as donor substrate, oligosaccharidyl-alditol as acceptor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan-derived nonasaccharide Glc4Xyl3GalFuc and certain other xyloglucan oligosaccharides, non-reducing terminal Xyl-Glc group is essential
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: high MW xyloglucan, xyloglucan-derived oligosaccharides with at least two alpha-D-xylose residues, reducing group is not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Pitcairnia imbricata
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: XXXG-ol, a reduced xyloglucan heptasaccharide, its dimer XXXGXXXG-ol, and other xyloglucan oligosaccharides, reaction probably involves an enzyme-donor-acceptor complex, in which the enzyme has two binding sites separately for donor and acceptor, mechanism
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme cleaves and religates xyloglucan polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
acceptors: sulforhodamine-labeled xyloglucan-oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Pothomorphe petalta
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Selaginella sp.
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Sinapis sp.
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
preference for solanaceous xyloglucan without Fuc over xyloglucan from other sources as donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
donor is apple xyloglycan, acceptor is XXXGol
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
Tragopodon pratensis
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptor: fucosylated xyloglucan nonasaccharide
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
XET is present in cell walls in form of a competent glycosyl-enzyme complex which decomposes by transglycosylation of its glycan moiety to added xyloglucan-oligosaccharide acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
2 isoenzymes: sXET from seeds acts on xyloglucan molecules stochastically along the length of their polyglucose main chain and prefers low-MW xyloglucan-derived oligosaccharides as acceptors, eXET from epicotyls attacks the substrate predominantly near the reducing end and shows no preference for a size of xyloglucan oligosaccharide acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
specific for xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
investigation of substrate subsite recognition requirements, xylose substitution at Glc +2 and -3 of the NXET cleavage site at the backbone of the xyloglucan substrate is a requirement, Gal substitution of a Xyl at +1 prevents, and at -2 modifies, chain-cleavage
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
2 distinct XETs with different substrate prefences, XET from epicotyl, XET1, uses nonfucosylated seed amyloid xyloglucan or fucosylated stem xyloglucan as substrate with equal facility, XET from cotyledon, NXG1, has a significantly higher activity against nonfucosylated xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme requires a basic xyloglucan structure, i.e. a beta-1,4-glucosyl backbone with xylosyl side chains, for both acceptor and donor activity, higher activity when xyloglucans with higher MW are used as donor substrates, xyloglucans smaller than 10 kDa are no donor substrates, pyridylamino heptasaccharide, xyloglucan oligomers or polymers are good acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Vigna angularis
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
donor xyloglucan cleavage sites of XTHs from stems and epicotyls
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
galactosyl or fucosyl side chains are not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
pronounced preference for xyloglucan oligosaccharides with backbones of 4 over 3 over 2 Glc residues as acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
XET forms a stable donor xyloglucan-XET complex
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
Apium sp.
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
Sinapis sp.
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
-
-
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
-
acceptor: xyloglucan-derived nonasaccharide XG9, Glc4Xyl3GalFuc, highly specific for xyloglucan as the glycosyl donor, xyloglucan from Tropaeolum seed is somewhat better than from Rosa cultures, enzyme transfers part of a large xyloglucan molecule to the nonasaccharide forming a polymer with a beta-1,4-linkage to the product and the acceptor group of the nonasaccharide is O-4 of the Glc residue furthest from the reducing terminus
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
-
XXFG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
-
XXFG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
-
XXFG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
XXFG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
-
TCH4 protein, 50% as effective as XLLG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: 25-30% as effective as XLLG-ol, less effective than XXXG-ol, XTR9: more effective than XXXG-ol, donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
-
Xyl2Glc3 is less effective than heptasaccharide XG7: Xyl3Glc4, minimal structure required for acceptor activity
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
-
XXG, preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
-
Xyl2Glc3 is less effective than heptasaccharide XG7: Xyl3Glc4, minimal structure required for acceptor activity
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc-ol
?
-
pentamer XXG-ol, all isoenzymes have low but significant activity, tamarind xyloglucan as donor
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc-ol
?
-
pentamer XXG-ol, all isoenzymes have low but significant activity, tamarind xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
TCH4 protein, better substrate than XXFG-ol, lower affinity than for polymer xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
reaction results in a hybrid product and a leaving group
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
1.7-3.6fold preference of XLLG-ol over XXXG-ol, mung bean isoenzymes have higher affinities for XLLG-ol than cauliflower isoenzymes
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
isoenzyme C45a dissociates from the reaction products after each polysaccharide-to-oligosaccharide transglycosylation event
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
1.7-3.6fold preference of XLLG-ol over XXXG-ol, mung bean isoenzymes have higher affinities for XLLG-ol than cauliflower isoenzymes
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
-
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XG9n, better substrate than Glc4Xyl3GalFuc
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XLLG from Tropaeolum majus seed, donor xyloglucan from tamarind cotyledons or pea stems, XET1 uses nonfucosylated tamarind seed amyloid xyloglucan or fucosylated pea stem xyloglucan as substrate with equal facility, NXG1 has a significantly higher activity against nonfucosylated tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
Glc4Xyl3Gal2
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
heptasaccharide XG7, Xyl3Glc4, is more effective than Xyl2Glc3
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
heptasaccharide XG7, Xyl3Glc4, is more effective than Xyl2Glc3
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XG7, better substrate than Glc4Xyl3GalFuc
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
-
XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: more effective than XXFG-ol, XTR9: less effective than XXFG-ol, donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
isoenzymes have 9-19times greater activities than on XXG-ol, reduced activity with XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
pine or tobacco xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XG7-ol is approximately as effective as heptasaccharide XG7, efficient substrate, reducing group is not required for acceptor activity
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
-
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
pea xyloglucan as donor, specific for xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
transfers a part of xyloglucan to the reduced xyloglucan heptasaccharide XXXG-ol, the acceptor acts by combining with the enzyme independently of the donor, the velocity of the reaction decreases gradually as the heptasaccharide unit is increased from two to four, the affinity for XXXG-ol is increased at a higher concentration of donor xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
isoenzymes have 9-19times greater activities than on XXG-ol, reduced activity with XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA
?
-
pyridylamino XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA
?
-
pyridylamino heptasaccharide is a good substrate
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Adiantum capillus-veneri
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Aloe sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Anthurium upalaense
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
the xyloglucan:xyloglucosyl transferase gene TCH4 is strongly upregulated by environmental stimuli, enzyme may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain, TCH4 protein may contribute to the adaptive changes in morphogenesis that occurs in the organism following exposure to mechanical stimuli
-
-
?
donor xyloglucan + xyloglucan acceptor
?
XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in cell elongation
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Azolla sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Chamaecyparis sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Commelina nilagirica
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
key enzyme responsible for forming and rearranging the cellulose/xyloglucan network of the cell wall, commitment to the construction of both cell plate and cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme depolymerises xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme depolymerises xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
involved in xyloglucan metabolism
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Pitcairnia imbricata
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones
-
-
?
donor xyloglucan + xyloglucan acceptor
?
multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Pothomorphe petalta
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Selaginella sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network
-
-
?
donor xyloglucan + xyloglucan acceptor
?
enzyme mediates cell wall disassembly associated with expansive growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Tragopodon pratensis
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
2 isoenzymes: sXET from seeds plays a role in degrading xyloglucan reserves in seeds during germination, eXET from epicotyls is engaged in cell wall rearrangement and integration of new xyloglucan molecules into the preexisting cell wall structure during growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in elongation-growth and other processes involving xyloglucan metabolism
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of four enzymes, which act in concert to catalyze the mobilisation of storage xyloglucan from the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
XET1 may play a role in cell-wall xyloglucan metabolism, such as the incorporation of newly synthesized xyloglucan into the expanding primary cell wall or the modification of xyloglucan polymers forming cross-links between cellulose microfibrils, NXG1 in vivo predominantly exhibits xyloglucanase activity and mobilizes nonfucosylated xyloglucan seed storage reserves
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in wall assembly as well as loosening
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
-
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
-
-
-
?
tamarind xyloglucan + XXXGol
?
the enzyme performs transglucosylation
-
-
?
xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
-
-
-
-
?
xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
-
-
-
-
?
xyloglucan nonasaccharide + xyloglucan
?
acceptor: nonasaccharide XLLGol, donor: tamarind xyloglucan
-
-
?
xyloglucan nonasaccharide + xyloglucan
?
nonasaccharide XLLG, a 2-chloro-4-nitrophenyl-nonasaccharide is no substrate, tamarind xyloglucan
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
-
sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
-
sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
-
sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
Lactuca sativa ssp. capitata
-
sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
-
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
Piper sp.
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sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
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xyloglucan oligosaccharides + xyloglucan
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sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
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xyloglucan oligosaccharides + xyloglucan
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sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
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[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
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[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
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additional information
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in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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not: carboxymethylcellulose, barley glucan
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additional information
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contains strongly conserved sequence motif of XETs: DEIDFEFLG
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additional information
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contains strongly conserved sequence motif of XETs: DEIDFEFLG
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additional information
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free oligosaccharides are probably not the usual acceptor substrates in vivo
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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anionic, oxidised derivatives of xyloglucan are used as substrates for AtXTH24
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additional information
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isozyme XTH14 uses donor substrates in order of preference xyloglucan >> watersoluble cellulose acetate > hydroxyethylcellulose > mixed-linkage beta-glucan > carboxymethylcellulose > methylcellulose, thus, all nonxyloglucan substrates show very low activity rates in comparison to xyloglucan. Isozyme XTH26 is able to use watersoluble cellulose acetate at a low rate but shows no activity with any of the other non-xyloglucan substrates tested
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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isozyme XHT31 shows predominant undetectable xyloglucan endohydrolase, XEH, activity and has only slight xyloglucan endotransglucosylase, XET, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. Isozyme XTH31's XET activity is strongly compromised when the second Xyl residue is galactosylated. XTH31 cleaves the substrate from about 186 kDa to 6 kDa (median sizes) within 40 h, gas spectrometric analysis. The isozyme does not have a preferred cleavage site close to either terminus. The complete hydrolysis of a 186-kDa tamarind xyloglucan molecule to yield Glc4-based oligosaccharides would require about 150 cuts. XEH action continues for at least 40 h, with an initial rate of about 1.5 cuts per starting molecule per hour. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview. The acceptor site of XTH31 is relatively poor at accommodating substrates that have Gal (or Fuc-Gal) simultaneously on the 2nd and 3rd isoprimeverose groups, counted from the nonreducing end
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additional information
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isozyme XHT31 shows predominant undetectable xyloglucan endohydrolase, XEH, activity and has only slight xyloglucan endotransglucosylase, XET, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. Isozyme XTH31's XET activity is strongly compromised when the second Xyl residue is galactosylated. XTH31 cleaves the substrate from about 186 kDa to 6 kDa (median sizes) within 40 h, gas spectrometric analysis. The isozyme does not have a preferred cleavage site close to either terminus. The complete hydrolysis of a 186-kDa tamarind xyloglucan molecule to yield Glc4-based oligosaccharides would require about 150 cuts. XEH action continues for at least 40 h, with an initial rate of about 1.5 cuts per starting molecule per hour. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview. The acceptor site of XTH31 is relatively poor at accommodating substrates that have Gal (or Fuc-Gal) simultaneously on the 2nd and 3rd isoprimeverose groups, counted from the nonreducing end
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additional information
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isozyme XHT31 shows predominant undetectable xyloglucan endohydrolase, XEH, activity and has only slight xyloglucan endotransglucosylase, XET, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. Isozyme XTH31's XET activity is strongly compromised when the second Xyl residue is galactosylated. XTH31 cleaves the substrate from about 186 kDa to 6 kDa (median sizes) within 40 h, gas spectrometric analysis. The isozyme does not have a preferred cleavage site close to either terminus. The complete hydrolysis of a 186-kDa tamarind xyloglucan molecule to yield Glc4-based oligosaccharides would require about 150 cuts. XEH action continues for at least 40 h, with an initial rate of about 1.5 cuts per starting molecule per hour. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview. The acceptor site of XTH31 is relatively poor at accommodating substrates that have Gal (or Fuc-Gal) simultaneously on the 2nd and 3rd isoprimeverose groups, counted from the nonreducing end
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additional information
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isozyme XTH15 has high xyloglucan endotransglucosylase, XET, and undetectable xyloglucan endohydrolase, XEH, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. XTH15's XET activity tolerateds substitution at the second Xyl residue. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview
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additional information
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isozyme XTH15 has high xyloglucan endotransglucosylase, XET, and undetectable xyloglucan endohydrolase, XEH, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. XTH15's XET activity tolerateds substitution at the second Xyl residue. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview
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additional information
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isozyme XTH15 has high xyloglucan endotransglucosylase, XET, and undetectable xyloglucan endohydrolase, XEH, activity in vitro. Usage of XXXG-ol as acceptor substrate and of xyloglucan as donor substrate. XXXG-ol is the preferred XET acceptor substrate, among five cellotetraitol-based oligosaccharides tested. XTH15's XET activity tolerateds substitution at the second Xyl residue. Substrate specificity of Arabidopsis thaliana XET isozymes XTH15 and XTH31, overview
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additional information
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enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
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additional information
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enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
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additional information
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evaluation of the substrate specificities of xyloglucan acceptors by using a set of synthetic oligosaccharides obtained by automated glycan assembly. The ability of XETs to incorporate the oligosaccharides into polysaccharides printed as microarrays and into stem sections is assessed, showing that single xylose substitutions are sufficient for transfer, and xylosylation of the terminal glucose residue is not required by XETs, independent of plant species. To obtain information on the potential xylosylation pattern of the natural acceptor of XETs, that is, the nonreducing end of xyloglucan, the activity of xyloglucan xylosyl transferase (XXT) 2 on the synthetic xyloglucan oligosaccharides is tested. Acceptor substrate specificities of XET, overview
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additional information
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[3H]XXXGol and tamarind xyloglucan are used as substrates in a radioactive enzyme assay to yield [3H]polysaccharide
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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free oligosaccharides are probably not the usual acceptor substrates in vivo
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additional information
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acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth
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additional information
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different assay methods, overview
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additional information
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different assay methods, overview
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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incorporation of sulforhodamine-labelled xyloglucan oligosaccharides (XyGO-SR) into xyloglucan
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH1
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH1
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH1
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH2
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH2
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additional information
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no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH2
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additional information
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when expressed in vitro, the recombinant DkXTH8 protein exhibits strict xyloglucan endotransglycosylase activity (XET, EC 2.4.1.207), whereas no xyloglucan endohydrolase activity (XEH, EC 3.2.1.151) is observed
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additional information
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XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
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additional information
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XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
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additional information
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enzyme predominantly catalyzes hetero-transglycosylation. Its preferred donor substrates (cellulose or mixed-linkage beta-glucan) differ qualitatively from its acceptor substrate xyloglucan. HTG generates stable cellulose-xyloglucan and mixed-linkage beta-glucan-xyloglucan covalent bonds. No donor substrate: cellohexaose
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additional information
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protein-ligand interaction analysis and mode of FcXTH2, overview. Favorable binding energies are obtained between FcXTH2 and the different ligands. Nevertheless, the interaction of FcXTH2 with XXXGXXXG is highly favored, and a weaker binding interaction is found between the protein and cellodextrin 8-mer or XXFGXXFG as ligands. Different octasaccharides are positioned along the open groove of the protein. Only XXXGXXXG hemicellulose octasaccharide locates close to two of the catalytic residues (Glu85, Asp87) being able to interact with them
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additional information
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protein-ligand interaction analysis and mode of FcXTH2, overview. Favorable binding energies are obtained between FcXTH2 and the different ligands. Nevertheless, the interaction of FcXTH2 with XXXGXXXG is highly favored, and a weaker binding interaction is found between the protein and cellodextrin 8-mer or XXFGXXFG as ligands. Different octasaccharides are positioned along the open groove of the protein. Only XXXGXXXG hemicellulose octasaccharide locates close to two of the catalytic residues (Glu85, Asp87) being able to interact with them
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additional information
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enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE, EC 2.4.1.B52), cellulose:xyloglucan endotransglucosylase (CXE, EC 2.4.1.B52), and xyloglucan endotransglucosylase (XET) activities
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additional information
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no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin, and esterified pectin (potassium salt), orange polygalactouronic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, beta-D-galactans, and arabinogalactan protein with xyloglucan oligosaccharide and cellooligosaccharide, no transferase activity is detected laminari-oligosaccharides as acceptor substrates
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additional information
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no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic)
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additional information
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no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic)
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additional information
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no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic)
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additional information
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no hydrolytic activity can be detected with either isoenzyme
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additional information
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no hydrolytic activity can be detected with either isoenzyme
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additional information
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no hydrolytic activity can be detected with either isoenzyme
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additional information
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no transferase activity is detected with laminarin, lichenin, pustulan, barley arabinoxylan, glucomannan, citrus fruit pectin or esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, lupin or potato beta-D-galactans, or gum arabic
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additional information
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XET5 catalyzes the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates and between xyloglucans and (1,3 1,4)-beta-D-glucans
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate and [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp as acceptor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate and [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp as acceptor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate and [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp as acceptor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate and [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp as acceptor substrate
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate. Transglycosylation reactions are catalysed by HvXET6 during a two-stage reaction cycle
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate. Transglycosylation reactions are catalysed by HvXET6 during a two-stage reaction cycle
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate. Transglycosylation reactions are catalysed by HvXET6 during a two-stage reaction cycle
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additional information
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xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate. Transglycosylation reactions are catalysed by HvXET6 during a two-stage reaction cycle
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additional information
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Lactuca sativa ssp. capitata
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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free oligosaccharides are probably not the usual acceptor substrates in vivo
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
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usage of xyloglucan heptasaccharide XXXG and its reduced form XXXGol as donor substrates
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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Q5Z6H1
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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Q6ZAN9
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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Petroselinum sp.
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usage of cellulose-bound tamarind seed xyloglucan as donor substrate and [1-3H]XXXGol as acceptor substrate
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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the overall xyloglucan-degrading activity in the presence and absence of XG subunit oligosaccharides, donor substrate is tamarind xyloglucan
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additional information
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prediction of union type and energy stability of the complexes formed between PrXTH1 and different substrates (XXXGXXXG, XXFGXXFG, XLFGXLFG and cellulose), structures overview. Molecular docking, molecular dynamics, MM-GBSA and electrostatic potential calculations are employed to predict the binding modes, free energies of interaction, and the distribution of electrostatic charge. The results suggest that the enzyme forms more stable complexes with hemicellulose substrates than cellulose, and the best ligand is the xyloglucan XLFGXLFG. PrXTH1 is able to interact with different xyloglycans structures but no activity is observed for cellulose as substrate
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additional information
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Piper sp.
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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oligosaccharides of cello-, chito- and/or oligoglucurono-series are much less effective than xyloglucan-derived oligosaccharides
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additional information
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no acceptors: alpha-D-Xylp(1-6)D-Glc and isoprimeverose
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additional information
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the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
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additional information
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evaluation of the substrate specificities of xyloglucan acceptors by using a set of synthetic oligosaccharides obtained by automated glycan assembly. The ability of XETs to incorporate the oligosaccharides into polysaccharides printed as microarrays and into stem sections is assessed, showing that single xylose substitutions are sufficient for transfer, and xylosylation of the terminal glucose residue is not required by XETs, independent of plant species. To obtain information on the potential xylosylation pattern of the natural acceptor of XETs, that is, the nonreducing end of xyloglucan, the activity of xyloglucan xylosyl transferase (XXT) 2 on the synthetic xyloglucan oligosaccharides is tested. Acceptor substrate specificities of XET, overview
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additional information
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substrate specificity of isozymes PtoXTH27 and PtoXTH34, PtoXTH34 has a higher affinity for XXXG. The receptor substrate of XTH in plants may be XXXG, or xyloglucan oligosaccharides with different chain lengths or structures, such as XLLG and XXXGXXXG. The different affinities of PtoXTH34 and PtoXTH27 for XXXG may be due to the diversity of receptor substrates in plants. Additionally, PtoXTH27 may have other receptor substrates with higher affinity
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additional information
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substrate specificity of isozymes PtoXTH27 and PtoXTH34, PtoXTH34 has a higher affinity for XXXG. The receptor substrate of XTH in plants may be XXXG, or xyloglucan oligosaccharides with different chain lengths or structures, such as XLLG and XXXGXXXG. The different affinities of PtoXTH34 and PtoXTH27 for XXXG may be due to the diversity of receptor substrates in plants. Additionally, PtoXTH27 may have other receptor substrates with higher affinity
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additional information
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substrate specificity of isozymes PtoXTH27 and PtoXTH34, PtoXTH34 has a higher affinity for XXXG. The receptor substrate of XTH in plants may be XXXG, or xyloglucan oligosaccharides with different chain lengths or structures, such as XLLG and XXXGXXXG. The different affinities of PtoXTH34 and PtoXTH27 for XXXG may be due to the diversity of receptor substrates in plants. Additionally, PtoXTH27 may have other receptor substrates with higher affinity
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additional information
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contains strongly conserved sequence motif of XETs: DEIDFEFLG
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additional information
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PttXET16A has 4 conserved Cys residues
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additional information
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key enzyme in all plant processes that require cell wall remodeling
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additional information
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key enzyme in all plant processes that require cell wall remodeling
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additional information
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no hydrolytic activity of the wild-type or N93S mutant enzyme
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additional information
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transglycosylation acceptor binding mechanism, structure-function study
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additional information
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transglycosylation acceptor binding mechanism, structure-function study
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additional information
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does not produce any hetero-transglycosylation products with cello-oligosaccharides or lactose
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1-4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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activity assay with reduced xyloglucan-derived nonasaccharide as substrate
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additional information
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enzyme activity assay with partially purified tamarind xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
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additional information
?
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXG-ol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
enzyme activity assay with partially purified apple xyloglucan as donor and [3H]XXXGol as xyloglucan acceptor substrate
-
-
?
additional information
?
-
-
protein swollenin (SWOI) does not exhibit xyloglucan:xyloglucosyl transferase (XET) activity with donor substrate tamarind xyloglucan and and xyloglucan oligomers
-
-
?
additional information
?
-
-
NXET has also a xyloglucan-specific hydrolase activity, cleavage sites for hydrolysis and endotransglycosylation are identical, hydrolase action is significantly only under conditions, where suitable acceptor chain-end concentrations are limiting
-
-
?
additional information
?
-
-
no action on other poly- and oligosaccharides than xyloglucan, such as cellulose, its soluble derivatives, mixed linkage beta-glucans, cello-oligosaccharides, enzyme catalyzes, additional to the powerful and specific xyloglucan endo-transglycosylase action, the hydrolysis of tamarind xyloglucan to a mixture of oligosaccharides of different sizes, hydrolysis increases at low substrate concentrations
-
-
?
additional information
?
-
-
oligosaccharides of cello-, chito- and/or oligoglucurono-series are much less effective than xyloglucan-derived oligosaccharides
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
-
isozymes sXET and eXET show different specificities for xyloglucan-derived oligosaccharide acceptor substrates, and distinct preferences in selecting the site of attack on xyloglucan molecules
-
-
?
additional information
?
-
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
?
additional information
?
-
-
the enzyme exhibits broad substrate specificity by transferring xyloglucan or hydroxyethylcellulose fragments not only to oligoxyloglucosides and cellooligosaccharides but also to oligosaccharides derived from beta-(1,4)-D-glucuronoxylan, beta-(1,6)-D-glucan, mixed-linkage beta-(1,3 1,4)-D-glucan and at a relatively low rate also to beta-(1,3)-gluco-oligosaccharides
-
-
?
additional information
?
-
SR-labelled xyloglucan-oligosaccharides, laminari-oligosaccharides, mixed-linkage (1,3/1,4)-beta-D-glucosaccharides, (1,4)-beta-D-glucurono-xylooligosaccharides, cello-oligosaccharides, manno-oligosaccharides and penta-galacturonic acid oligosaccharide, are used as acceptors. Preferred substrate are xyloglucan-oligosaccharides and cello-oligosaccharides, no activity is detected in tissues other than germinating seeds with laminari-oligosaccharides, mixed-linkage (1,3/1,4)-beta-D-glucosaccharides, (1,4)-beta-D-glucurono-xylooligosaccharides, manno-oligosaccharides and penta-galacturonic acid oligosaccharide. Heterotransglycosylation activities and acceptor substrate specificity of TmXET6.3, overview
-
-
-
additional information
?
-
-
SR-labelled xyloglucan-oligosaccharides, laminari-oligosaccharides, mixed-linkage (1,3/1,4)-beta-D-glucosaccharides, (1,4)-beta-D-glucurono-xylooligosaccharides, cello-oligosaccharides, manno-oligosaccharides and penta-galacturonic acid oligosaccharide, are used as acceptors. Preferred substrate are xyloglucan-oligosaccharides and cello-oligosaccharides, no activity is detected in tissues other than germinating seeds with laminari-oligosaccharides, mixed-linkage (1,3/1,4)-beta-D-glucosaccharides, (1,4)-beta-D-glucurono-xylooligosaccharides, manno-oligosaccharides and penta-galacturonic acid oligosaccharide. Heterotransglycosylation activities and acceptor substrate specificity of TmXET6.3, overview
-
-
-
additional information
?
-
-
no glycosidase or glycanase activity, no donors: xyloglucans smaller than 10 kDa, carboxymethylcellulose, Avena glucan, maize xylan, Rhodymenia xylan, no acceptors: pyridylamino cellohexaose, pyridylamino laminarihexaose
-
-
?
additional information
?
-
-
xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan oligosaccharide degrading enzyme activity
-
-
?
additional information
?
-
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
xyloglucan oligosaccharides + xyloglucan
? + xyloglucan
-
xyloglucans with MW of about 600-1000 kDa
MW about 50 kDa
-
?
donor xyloglucan + xyloglucan acceptor
?
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Adiantum capillus-veneri
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Aloe sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Anthurium upalaense
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
the xyloglucan:xyloglucosyl transferase gene TCH4 is strongly upregulated by environmental stimuli, enzyme may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain, TCH4 protein may contribute to the adaptive changes in morphogenesis that occurs in the organism following exposure to mechanical stimuli
-
-
?
donor xyloglucan + xyloglucan acceptor
?
XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in cell elongation
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Azolla sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Chamaecyparis sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Commelina nilagirica
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
key enzyme responsible for forming and rearranging the cellulose/xyloglucan network of the cell wall, commitment to the construction of both cell plate and cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme depolymerises xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme depolymerises xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
involved in xyloglucan metabolism
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Pitcairnia imbricata
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones
-
-
?
donor xyloglucan + xyloglucan acceptor
?
multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Pothomorphe petalta
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Selaginella sp.
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network
-
-
?
donor xyloglucan + xyloglucan acceptor
?
enzyme mediates cell wall disassembly associated with expansive growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
Tragopodon pratensis
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
2 isoenzymes: sXET from seeds plays a role in degrading xyloglucan reserves in seeds during germination, eXET from epicotyls is engaged in cell wall rearrangement and integration of new xyloglucan molecules into the preexisting cell wall structure during growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in elongation-growth and other processes involving xyloglucan metabolism
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of four enzymes, which act in concert to catalyze the mobilisation of storage xyloglucan from the cell wall
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
XET1 may play a role in cell-wall xyloglucan metabolism, such as the incorporation of newly synthesized xyloglucan into the expanding primary cell wall or the modification of xyloglucan polymers forming cross-links between cellulose microfibrils, NXG1 in vivo predominantly exhibits xyloglucanase activity and mobilizes nonfucosylated xyloglucan seed storage reserves
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
existence of different classes of XET with differing roles in vivo
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
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-
?
donor xyloglucan + xyloglucan acceptor
?
-
associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
acts in root development, necessary for root hair growth
-
-
?
donor xyloglucan + xyloglucan acceptor
?
-
role in wall assembly as well as loosening
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
mixed-linkage (1->3,1->4)-beta-D-glucan
xyloglucan oligosaccharide
-
-
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
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-
?
additional information
?
-
-
acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth
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-
?
additional information
?
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breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
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?
additional information
?
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XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
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-
?
additional information
?
-
-
XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
key enzyme in all plant processes that require cell wall remodeling
-
-
?
additional information
?
-
-
key enzyme in all plant processes that require cell wall remodeling
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1-4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
-
xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan oligosaccharide degrading enzyme activity
-
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
?
additional information
?
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Al3+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Ca2+
CaCl2
-
the ability of cold-water-extractable, heat-stable polymers (CHPs) to solubilize XTHs from Arabidopsis cell walls is mimicked by NaCl and CaCl2. The effect plateaues above about 30 mM CaCl2, while the CHP effect does not plateau even at the highest concentration tested (1.8 mg/ml). The relative effect of CHP is greatest (34fold promotion) in the absence of CaCl2, but strong CHP effects (4.6 to 9.1fold promotion) and much higher absolute XET activities are still detected in the presence of 15 mM CaCl2, indicating synergy between CHP and the inorganic salt
La3+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Mg2+
NaCl
additional information
Ca2+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Mg2+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
NaCl
induces an increase in XTH30 expression and xyloglucan (XyG)-derived oligosaccharides (XLFG) in the wild-type
NaCl
-
the ability of cold-water-extractable, heat-stable polymers (CHPs) to solubilize XTHs from Arabidopsis cell walls is mimicked by NaCl and CaCl2
additional information
-
TCH4 protein is not activated by Ca+ or other divalent cations
additional information
-
the enzyme is activated by a wide variety of inorganic and organic salts
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-([3-(4-hydroxyphenyl)-3-oxopropanoyl]amino)-4-[methyl(octadecyl)amino]-benzoic acid
Petroselinum sp.
-
-
Coomassie Brilliant Blue R250
-
i.e. BB-R250, the parasite shows no preference towards inhibitor-coated petioles or control petioles, readily starting to develop haustoria on any of them. Sectioning and microscopical analysis of infection sites, that have progressed to the mature stage, reveal that approximately 1/3 of haustoria on 5 mM BB-R250-coated petioles have not been able to grow into the host tissue
N'-(1-E)-(5-bromofuran-2-yl)-methylidene-2-(3-methylphenoxy)-acetohydrazide
Petroselinum sp.
-
-
riboflavin
Petroselinum sp.
-
the riboflavin inhibitory effect is light-dependent, non-specific
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
significant substrate inhibition of isoenzyme XTR9 at high concentrations; slight substrate inhibition of isoenzymes Meri-5 and EXGT at high concentrations
additional information
-
the xyloglucan endoglucanase (XEG) shows moderate inhibition of XTH-activating factor (XAF) activity. Cauliflower CHP exerts its XAF activity principally by (re-)solubilizing XTHs from surfaces (including cellulose, glass-fibre, glass and plastics) to which these enzymes tend to bind
-
additional information
-
not inhibited by 1 M fluoride, 1 M urea, 1 M DMSO, 300 mM borate, 100 mM EDTA, 100 mM Triton X-100
-
additional information
glycosylation is required for activity, deglycosylation results in 40% loss of activity
-
additional information
-
glycosylation is required for activity, deglycosylation results in 40% loss of activity
-
additional information
Petroselinum sp.
-
high-throughput fluorescent dot-blot screening to search for enzyme inhibitors in vitro, detailed overview. Of 4216 xenobiotics tested, with cellulose-bound xyloglucan as donor-substrate, 18 inhibit XET activity and 18 promote it (especially anthraquinones and flavonoids). No compounds promotes XET in quantitative assays with (cellulose-free) soluble xyloglucan as substrate, suggesting that promotion is dependent on enzyme-cellulose interactions. With cellulose-free xyloglucan as substrate, 22 XET-inhibitors are identified, especially compounds that generate singlet oxygen. Inhibitors include tannins, sulphydryl reagent,s and triphenylmethanes
-
additional information
-
not inhibited by 10 mM D-gluconolactone, chelating agents, cello-oligosaccharides, cellopentaose
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
additional information
-
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acidic arabinogalactan protein
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MW about 5000 Da, acidic polymers and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth, composition overview
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bovine serum albumin
-
enhances activity by stabilizing TCH4 protein conformation
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Gum arabic
-
from Acacia sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
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hypochlorite-oxidized xyloglucan
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from tamarind, 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
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Pectin
-
from Citrus sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
xyloglucan oligosaccharides
-
xyloglucan oligosaccharides enhance enzyme activity
xyloglucan-derived oligosaccharide
-
stimulates, highest stimulating effect with the nonasaccharide Glc4Xyl3Gal2, lowest one with the pentasaccharide Glc3Xyl2
XTH-activating factor
XAF, present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins
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XTH-activating factor
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an endogenous cold-water-extractable, heat-stable polymer(s) (CHP) from cauliflower florets acts as an XTH-activating factor (XAF), promoting the XET activity of XTHs. The XTH-activating factors from plants desorb and thereby activate wall-bound XTHs. CHP specifically desorbed wall-bound XTHs, but not beta-glucosidases, phosphatases or peroxidases. CHP preparations from 25 angiosperms all possess XAF activity but have no consistent monosaccharide composition. Of 11 individual plant polymers tested, only gum arabic and tamarind xyloglucan are XAF-active, albeit less so than CHP. On gel-permeation chromatography, XAF-active cauliflower CHP elutes with a molecular weight of 7000-140000, although no specific sugar residue(s) co-elute exactly with XAF activity. Cauliflower XAF activity survives cold alkali and warm dilute TFA (which break ester and glycofuranosyl linkages, respectively), but is inactivated by hot 2 M TFA (which breaks glycopyranosyl linkages). Cauliflower XAF activity is remarkably stable to diverse glycanases and glycosidases, sugar composition of XAF-active CHPs from diverse plants and evaluation of XAF activity, detailed overview. The ability of CHP to solubilize XTHs from Arabidopsis cell walls is mimicked by NaCl and CaCl2. The effect plateaues above about 30 mM CaCl2, while the CHP effect does not plateau even at the highest concentration tested (1.8 mg/ml). The relative effect of CHP is greatest (34fold promotion) in the absence of CaCl2, but strong CHP effects (4.6 to 9.1fold promotion) and much higher absolute XET activities are still detected in the presence of 15 mM CaCl2, indicating synergy between CHP and the inorganic salt. Xyloglucan endoglucanase (XEG) shows some (moderate) ability to inactivate XAF. CHP has a stronger XAF effect on more dilute cell-wall suspensions
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XTH-activating factor
XAF, present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins
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XTH-activating factor
XAF, present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins
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additional information
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TCH4 protein is not activated by reducing agents, spermidine, spermine, glycerol or carboxymethylcellulose
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additional information
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At-XTH22 is strongly induced in response to mechanical perturbations such as wind or touch
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additional information
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
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additional information
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no activation by carboxymethylcellulose, non-oxidized xyloglucan, homo-galacturonic acid, and starch, overview
-
additional information
glycosylation is required for activity, deglycosylation results in 40% loss of activity
-
additional information
-
glycosylation is required for activity, deglycosylation results in 40% loss of activity
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additional information
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
-
additional information
-
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
-
additional information
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strong positive correlation between gibberellic acid-enhanced length and extractable XET activity in internodes
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additional information
LeEXT gene encoding XET is induced by hormone treatments that elicites elongation of hypocotyl segments, e.g. treatment with auxin, brassinolide or 2,4-D
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additional information
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LeEXT gene encoding XET is induced by hormone treatments that elicites elongation of hypocotyl segments, e.g. treatment with auxin, brassinolide or 2,4-D
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additional information
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0209
alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)-D-Glc
pH 5.0, 37°C, recombinant enzyme
1.6
donor Glc8-based xyloglucan oligosaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.0386
XXXG-ol
xyloglucan endotransglucosylase activity, pH 5.5, 20°C
0.031
XXXGol
xyloglucan endotransglucosylase activity, pH 5.5, 20°C
0.019
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
-
-
0.005 - 0.13
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
0.033
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
-
-
0.1 - 0.32
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
0.23
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
XXXGXXXG-ol
0.0053 - 0.04
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.0086 - 0.0242
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.005 - 0.0106
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.062
nonasaccharide XLLG-ol
pH 5.5, 25°C, recombinant N93S mutant enzyme
0.075
nonasaccharide XLLG-ol
pH 5.5, 25°C, recombinant wild-type enzyme
0.0085
reduced xyloglucan-derived heptasaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.0399
reduced xyloglucan-derived heptasaccharide
purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.017
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol
-
isozyme eXET, pH 5.5, 30°C
0.131
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol
-
isozyme sXET, pH 5.5, 30°C
0.05
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
-
xyloglucan-derived nonasaccharide
0.005
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
isoenzyme XTR9
0.016
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M55a
0.017
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
isoenzyme TCH4
0.028
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M45
0.03
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
isoenzyme EXGT
0.031 - 0.035
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzymes M35a and M35b
0.032
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M55b
0.04
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
isoenzyme Meri-5
0.071 - 0.082
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzymes C45a and C45b
0.073
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
acceptor XLLG-ol, recombinant TCH4 protein
0.073
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
recombinant At-XTH22
0.13
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme C30
0.1
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan
0.32
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
XXXG-ol
1.4
xyloglucan
pH 5.5, 25°C, recombinant N93S mutant enzyme
2.2
xyloglucan
pH 5.5, 25°C, recombinant wild-type enzyme
0.0053
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.039
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.04
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.0086
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.0113
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.0242
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.005
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.0103
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.0106
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
additional information
additional information
-
Km: 0.6 mg/ml for kiwifruit xyloglucan
-
additional information
additional information
-
recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan
-
additional information
additional information
-
recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
purified AtXTH21 activity, Km: 0.6245 mg/ml
-
additional information
additional information
-
purified AtXTH21 activity, Km: 0.6245 mg/ml
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
-
Michaelis-Menten kinetics for AtXTH12, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
-
Michaelis-Menten kinetics for AtXTH13, overviews
-
additional information
additional information
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
-
double sigmoidal kinetics of isozyme from cv. Fuping Jianshi fruits
-
additional information
additional information
kinetics of 1.6 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
kinetics of 1.6 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
-
kinetics of 1.6 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
kinetics of 2.9 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
kinetics of 2.9 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
-
kinetics of 2.9 mg/ml with xyloglucan, xyloglucan endotransglucosylase activity. Comparisons of kinetics of isozyme XTH15 and XTH31 from Arabidopsis thaliana, overview
-
additional information
additional information
classical hyperbolic Michaelis-Menten curve for XET in the case of XTH15
-
additional information
additional information
classical hyperbolic Michaelis-Menten curve for XET in the case of XTH15
-
additional information
additional information
Km of the XET activity of XTH31 for tamarind xyloglucan cannot be estimated because a hyperbola is not obtained
-
additional information
additional information
Km of the XET activity of XTH31 for tamarind xyloglucan cannot be estimated because a hyperbola is not obtained
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.02
alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)-D-Glc
pH 5.0, 37°C, recombinant enzyme
4.8
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc
-
-
0.139
Glc8-based xyloglucan oligosaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.00031
pea xyloglucan
-
recombinant TCH4 protein, with XLLG-ol as acceptor
-
0.00018
tamarind xyloglucan
-
recombinant TCH4 protein, with XLLG-ol as acceptor
-
0.45
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc
-
-
0.007 - 0.17
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.005 - 0.12
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.005 - 0.058
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.0024
reduced xyloglucan-derived heptasaccharide
purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.0089
reduced xyloglucan-derived heptasaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.007
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.021
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.17
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.005
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.017
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.12
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.005
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.006
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.058
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
48.8
alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-[alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)]-beta-D-Glc-(1->4)-D-Glc
pH 5.0, 37°C, recombinant enzyme
0.0869
Glc8-based xyloglucan oligosaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.2 - 30
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.4 - 13
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.5 - 5
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
0.2
reduced xyloglucan-derived heptasaccharide
purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
1.1
reduced xyloglucan-derived heptasaccharide
recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.2
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.5
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
30
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.4
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
1
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
13
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
0.5
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
1
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
5
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.02
-
crude cell extract, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride
0.06
pH 5.5, 25°C, recombinant N93S mutant enzyme
60.51
-
after 3025fold purification, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride
additional information
additional information
Apium sp.
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
additional information
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
additional information
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
additional information
different assay methods, overview
additional information
-
low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously
additional information
-
low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously
additional information
-
low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously
additional information
-
after purification the concentration of enzymatically active XET is about 29 mg/l with a specific activity of 11 U/mg
additional information
Sinapis sp.
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
additional information
-
low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously
additional information
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 6.5
5 - 5.5
5.5
5.8
6 - 6.5
6.5
4.5 - 6.5
recombinant truncated isozyme
5 - 5.5
recombinant truncated isozyme
6
isozyme XTH15 exhibits an unusually broad pH optimum, inactive at pH 3.5 and below, xyloglucan endotransglucosylase activity
6
assay at
6
assay at
6
xyloglucan endotransglucosylase assay
6 - 6.5
isoenzymes TCH4, Meri-5, EXGT and XTR9, EXGT shows very sharp pH-optimum at pH 6
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2 - 8
recombinant enzyme, activity range, profile overview
3 - 8
recombinant enzyme, activity range, profile overview
4 - 6
isozyme DkXTH1 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs when the pH ranges from pH 4 to pH 5, which is typical for XET isoenzymes
4 - 7
4 - 8
4.5 - 6.5
pH-profile, rapid loss of activity below pH 4.5 and above pH 6.5
4.5 - 8
5 - 7
5 - 7.5
-
pH 5: about 30% of maximal activity, pH 7.5: about 40% of maximal activity
5.5 - 8
additional information
4 - 7
isozyme DkXTH2 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs below pH 4, which is typical for XET isoenzymes
4 - 7
over 30% of maximal activity within this range, recombinant enzyme, profile overview
4 - 8
-
both isoenzymes show a fast and symmetrical decrease in activity on both sides of their optima followed by an acidic shoulder between pH 4.0 and 5.0 for XTH14 and between pH 4.0 and 4.5 for XTH26. At pH 4.0 and 8.0, XTH14 retains about 30% and XTH26 40% of its maximal activity
4 - 8
AtXTH13 is more tolerant to basic environments, retaining 40% of its maximal activity at pH 8.0, but it displays only 20% activity at pH 4.0. The enzyme is inactive at pH 3.5. Bell-shaped curve of AtXTH13
4 - 8
bell-shaped curve of AtXTH12
4.5 - 8
XTR9 retains approximately 35% of its maximal activity at pH 4.5 and 30% at pH 8
4.5 - 8
TCH4 and Meri-5 retain approximately 10-15% of its maximal activity at pH 4.5 and 65% at pH 8
5.5 - 8
the activity-pH profile of AtXTH17 totally shifts towards the alkali side with its optimum at pH 7.2 and with an unusual acid intolerance. AtXTH18 shows the highest acid tolerance of these three enzymes. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0, whereas the activities of AtXTH17 and AtXTH19 drop below 30% of their maximal activity at pH 6.0. At or below pH 5.5, all three enzymes display less than 20% of their maximal activities. On the basic side, AtXTH17 shows the highest tolerance with activity of more than 80% of maximal activity at pH 8.0
5.5 - 8
the activity-pH profile of AtXTH18 totally shifts towards the alkali side with optimum at pH 7.5 and with an unusual acid intolerance. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0. At or below pH 5.5, the enzyme displays less than 20% of its maximal activity. On the basic side, AtXTH18 shows the highest tolerance with activities of more than 80% of its maximal activity at pH 8.0
5.5 - 8
the activity-pH profile of AtXTH19 totally shifts towards the alkali side with optimum at pH 7.0 and with an unusual acid intolerance. Activity of AtXTH19 drops below 30% of its maximal activity at pH 6.0. At or below pH 5.5, the enzymes displays less than 20% of its maximal activities. AtXTH19 retains 90% of its maximal activity at pH 7.5, but shows a 40% activity drop between pH 7.5 and pH 8.0
additional information
isozyme XTH31's hydrolase activity increases almost linearly with decreasing pH in the apoplastic range, pH6.2-4.5, consistent with a possible role in acid growth, inactive at pH 3.5 and below, xyloglucan endotransglucosylase activity
additional information
isozyme XTH31's hydrolase activity increases almost linearly with decreasing pH in the apoplastic range, pH6.2-4.5, consistent with a possible role in acid growth, inactive at pH 3.5 and below, xyloglucan endotransglucosylase activity
additional information
-
isozyme XTH31's hydrolase activity increases almost linearly with decreasing pH in the apoplastic range, pH6.2-4.5, consistent with a possible role in acid growth, inactive at pH 3.5 and below, xyloglucan endotransglucosylase activity
additional information
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity
additional information
-
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
22
24 - 34
25
27
30
37
additional information
25
assay at
25
assay at
25
xyloglucan endotransglucosylase assay
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0
-
the enzyme also operates efficiently at 0 °C, where it shows almost 40% of the activity observed at 30°C
10 - 50
about 20% of maximal activity at 10°C and 50°C, about 50% of maximal activity at 20°C and 48°C, temperature-profile
15 - 55
temperature-profile, about 20% of maximal activity at 15°C and 55°C
18 - 26
-
recombinant TCH4 protein, more active at lower temperatures, at or below 18°C, less active at higher temperatures, above 26°C
20 - 50
over 40% of maximal activity within this range, recombinant enzyme, profile overview
45
5
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5°C
45
isoenzymes EXGT and Meri-5 retain approximately 80% of maximum activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.4 - 9.6
-
at least 5 isozymes in cotyledons and leaves, development of a sensitive method for activity detection of isozymes in native isoelectric focusing gels, overview
4.7
calculated from amino acid sequence
4.8
5.9
calculated from amino acid sequence
6.1
calculated from amino acid sequence
6.4
calculated from amino acid sequence
6.7
6.8
calculated from amino acid sequence
6.9
calculated from amino acid sequence
7.5
calculated from amino acid sequence
7.7
calculated from amino acid sequence
8.2
calculated from amino acid sequence
8.3
8.5
8.66
9.2
9.28
9.4
9.8
calculated from amino acid sequence
additional information
4.8
calculated from amino acid sequence
6.7
calculated from amino acid sequence
8
calculated from amino acid sequence
8.3
calculated from amino acid sequence
8.3
calculated from amino acid sequence
9.2
calculated from amino acid sequence
9.2
calculated from amino acid sequence
additional information
-
at least 4 isozymes, development of a sensitive method for activity detection of isozymes in native isoelectric focusing gels, overview
additional information
-
at least 4 isozymes, development of a sensitive method for activity detection of isozymes in native isoelectric focusing gels, overview
additional information
comparison with 10 other XTH genes from Populus tomentosa, overview
additional information
comparison with 10 other XTH genes from Populus tomentosa, overview
additional information
-
comparison with 10 other XTH genes from Populus tomentosa, overview
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Apium sp.
i.e. Sinopodophyllum hexandrum, collected from the alpine zone of Koksar, Western Himalayan region, gene XET1
UniProt
brenda
ripe kiwifruit, [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward, 6 isoenzymes: AdXET1-6 gene family
-
-
brenda
3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5, EXGT belongs to XET class I, TCH4 and MERI-5 to XET class II
-
-
brenda
systematic nomenclature of the XTH gene family with 3 subfamilies
-
-
brenda
XETs are encoded by a gene family, 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9
-
-
brenda
XTR9, one of 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9
SwissProt
brenda
DcXTH1; four XTH genes DcXTH1-DcXTH4
UniProt
brenda
DcXTH2; four XTH genes DcXTH1-DcXTH4
UniProt
brenda
DcXTH3; four XTH genes DcXTH1-DcXTH4
UniProt
brenda
DcXTH4; four XTH genes DcXTH1-DcXTH4
UniProt
brenda
cv. Fuping Jianshi, cv. Ganmaokui
UniProt
brenda
cv. Fupingjianshi, i.e. Diospyros chinensis
UniProt
brenda
bifunctional xyloglucan endotransglucosylase and mixed-linkage glucan:xyloglucan endotransglucosylase, ratio of the activities is 1:7, respectively
UniProt
brenda
i.e. Pyrud malus, three apple cultivars, Fuji, Golden Delicious, and Taishanzaoxia
-
-
brenda
i.e. Pyrud malus, three apple cultivars, Fuji, Golden Delicious, and Taishanzaoxia
UniProt
brenda
cv. Samsun NN, NtXET-1, belongs to group I of XET-related subfamilies
SwissProt
brenda
isozymes XTH10, XTH11, XTH12, and XTH18; isozymes XTH10, XTH11, XTH12, and XTH18
SwissProt
brenda
isozymes XTH2-XTH4, XTH6, XTH7, XTH9, XTH13-XTH16, XTH19, XTH20, and XTH22-XTH29
-
-
brenda
hybrid aspen, poplar, major isoenzyme PttXET16A, a member of XET subfamily I
SwissProt
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cultivar Money Maker
UniProt
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SlXTH1, XTH1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
SwissProt
brenda
SlXTH10, tXET-B2, LetXET-B2; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH11, tXET-B1, LetXET-B1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH12, BR1 fragment, LeBR1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH2, LeXET2; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH3, XTH3; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH4, XET4; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH5, XTH5; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH6, XTH6; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH7, XTH7; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH8, ETAG-A3 fragment; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
SlXTH9, XTH9; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
brenda
-
-
-
brenda
var. Fiery Festival, 2 enzyme forms encoded by the genes XET1 and NXG1
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
brenda
ripe fruit
brenda
isozyme DkXTH2 reaches maximum expression concomitance with pronounced fruit softening
brenda
isozyme DkXTH3 reaches maximum expression concomitance with pronounced fruit softening
brenda
isozyme expression level during softening for 18-24 d using quantitative real-time PCR analysis. Isozymes DKXTH1 and DKXTH2 in untreated fruit have different expression patterns during fruit softening, in which maximum expression occurs on days 3 and 12 of ripening, respectively. 1-Methylcyclopropene (1-MCP) and gibberellic acid (GA3) treatments delay the softening and ethylene peak of persimmon fruit, as well as suppress the expression of both XTH genes, especially gene DKXTH1
brenda
isozyme expression level during softening for 18-24 d using quantitative realtime PCR analysis. Isozymes DKXTH1 and DKXTH2 in untreated fruit have different expression patterns during fruit softening, in which maximum expression occurs on days 3 and 12 of ripening, respectively. 1-Methylcyclopropene (1-MCP) and gibberellic acid (GA3) treatments delay the softening and ethylene peak of persimmon fruit, as well as suppress the expression of both XTH genes, especially gene DKXTH1
brenda
the expression of isozyme DkXTH1 is very high in immature growing fruit and peaks before the mature stage
brenda
the expression of isozyme DkXTH4 is very high in immature growing fruit and peaks before the mature stage
brenda
the expression of isozyme DkXTH5 is very high in immature growing fruit and peaks before the mature stage
brenda
expression analysis of isozymes DkXTH1-5 during Fuping Jianshi fruit development, expression patterns, overview
brenda
low expression level in young fruits, very low expression level in ripe fruits
brenda
lower expression level in young fruits, moderate to high expression level in ripe fruits
brenda
-
FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages
brenda
-
the expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages
brenda
ripe fruit
brenda
expression levels of isozymes, overview
brenda
-
brenda
-
brenda
LeEXT gene is expressed in outer cell layers of the hypocotyl, after auxin treatment overlapping spatial distribution in the epidermis and outer cortical cell layers
brenda
-
internodes I, II, III and V, numbered from the cotyledonary node
brenda
high expression level in young leaves, very high in older leaves
brenda
very high expression level in young leaves, low in older leaves
brenda
-
highest in green immature leaves as compared to maature leaves and brown, over-wintered leaves
brenda
-
FvXTH6 is expressed at a relatively high level in young leaf tissue but at a low level in old leaf tissue
brenda
-
brenda
source leaf, levels of NtXET-1 mRNA decreases in midribs with increasing age of leaves
brenda
-
highest level of transcripts of PtrXTH1 is detected in young, intensively growing leaves
brenda
PttXET16A is expressed transiently in developing leaves
brenda
-
XET1 is expressed in young and mature leaves at lower levels than in young epicotyls and roots, 7fold higher expression in young leaves than in mature ones
brenda
DcXTH2 transcript is detected in large quantities in petals as compared with other tissues
brenda
DcXTH3 transcript is detected in large quantities in petals as compared with other tissues
brenda
-
DcXTH3 transcript is detected in large quantities in petals as compared with other tissues
-
brenda
-
DcXTH2 transcript is detected in large quantities in petals as compared with other tissues
-
brenda
Apium sp.
-
activity is very pronounced in the vicinity of the vascular bundles
brenda
XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A
brenda
Adiantum capillus-veneri
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Aloe sp.
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Anthurium upalaense
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH12 is trichoblast-enriched
brenda
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH13 is trichoblast-enriched
brenda
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH17 is expressed in all cell types in the elongating and differentiating regions of the root
brenda
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH18 is expressed in all cell types in the elongating and differentiating regions of the root
brenda
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH19 is expressed in nearly all cell types in the root
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Azolla sp.
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Chamaecyparis sp.
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Commelina nilagirica
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Pitcairnia imbricata
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
PttXET16A expression in young roots and root tips
brenda
Pothomorphe petalta
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Selaginella sp.
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
Tragopodon pratensis
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
brenda
comparisons of mRNA populations of Arabidopsis root hair cells and cells of an Arabidopsis root hair mutant show AtXTH12 to be 4fold upregulated in root hair cells
brenda
accumulation of XET/H transcripts during seed germination, increased expression of PhXET influences seed germination in Podophyllum, expression analysis, overview
brenda
-
sprouting seedlings, more abundant in the growing tissues of the hypocotyls and leaves than in the cotyledons
brenda
-
MXE, EC 2.4.1., and XET, actions are both detectable in living Equisetum fluviatile shoots, the MXE:XET ratio increasing with age
brenda
-
etiolated stem, activity is positively correlated with growth rate in different zones of pea stem
brenda
-
XET1 is expressed at lower levels in stems than in young epicotyls and roots
brenda
XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A
brenda
-
developing, at least 16 Populus XTH genes are expressed in developing wood. Five genes are highly and ubiquitously expressed, whereas PtxtXET16-34 is expressed more weakly but specifically in developing wood
brenda
-
primary- and secondary-walled xylem tissues
brenda
additional information
-
Ac(MD2)XTH15 shows the highest relative expression level in the green leaf tip, sequentially in the root, and finally in the white leaf base
brenda
additional information
-
XETs accumulates in expanding cells, at the sites of intercellular airspace, formation, and at the bases of leaves, cotyledons and hypocotyls, detailed localization
brenda
additional information
isozyme XTH15 is predominantly expressed in early-stage organs
brenda
additional information
isozyme XTH15 is predominantly expressed in early-stage organs
brenda
additional information
-
isozyme XTH15 is predominantly expressed in early-stage organs
brenda
additional information
isozyme XTH31 is predominantly expressed in early-stage organs
brenda
additional information
isozyme XTH31 is predominantly expressed in early-stage organs
brenda
additional information
-
isozyme XTH31 is predominantly expressed in early-stage organs
brenda
additional information
enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels)
brenda
additional information
enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels)
brenda
additional information
enzyme XTH31 is strongly expressed in young, rapidly growing organs
brenda
additional information
enzyme XTH31 is strongly expressed in young, rapidly growing organs
brenda
additional information
XTH30 is highly expressed in the root, flower, stem, and etiolated hypocotyl. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves, real-time PCR enzyme expression analysis, expression pattern, overview
brenda
additional information
-
XTH30 is highly expressed in the root, flower, stem, and etiolated hypocotyl. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves, real-time PCR enzyme expression analysis, expression pattern, overview
brenda
additional information
-
XTH30 is highly expressed in the root, flower, stem, and etiolated hypocotyl. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves, real-time PCR enzyme expression analysis, expression pattern, overview
-
brenda
additional information
-
the level of xyloglucan endotransglucosylation (XET) activity peaks at the penetrating stage of Cuscuta reflexa on its host Pelargonium zonale
brenda
additional information
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
-
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
-
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
brenda
additional information
transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
brenda
additional information
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transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
brenda
additional information
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transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
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brenda
additional information
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transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR
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brenda
additional information
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transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
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brenda
additional information
isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
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isozymes DkXTH1, DkXTH4, and DkXTH5 peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage, while isozymes DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening, quantitative real-time PCR expression analysis, overview
brenda
additional information
transcriptional profiling reveals that DkXTH8 peaks during dramatic fruit softening. Very lo expression levels in flowers, calyces, leaves and stems, respectively
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additional information
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total extracts of Equisetum arvense organs exhibit XET activity, but vegetative lateral shoot extracts of contain somewhat less XET activity
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enzyme FcXTH2 shows a high expression level in vegetative tissues and a relatively low but constant expression level in developing fruit
brenda
additional information
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enzyme FcXTH2 shows a high expression level in vegetative tissues and a relatively low but constant expression level in developing fruit
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additional information
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to confirm the transcriptome data, the expression pattern of FvXTH6 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. The expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages. The absolute expression level of FvXTH6 in fruit is much lower than that of FvXTH9. FvXTH6 is also highly expressed in flowers. FvXTH6 is expressed at relatively high level in young leaf tissue but at low level in old leaf tissue
brenda
additional information
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to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles
brenda
additional information
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the three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, overview. Almost no XTH expression in roots
brenda
additional information
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the three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, overview. Almost no XTH expression in roots
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brenda
additional information
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expression profiles based on the barley genome database show that HvXTH family members display different expression patterns in different tissues and at different stages. Transcript levels of HvXTH isozymes in different tissues, overview
brenda
additional information
tissue-specific NtXET-1 expression pattern, highest mRNA levels in organs highly enriched in vascular tissues
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additional information
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tissue-specific NtXET-1 expression pattern, highest mRNA levels in organs highly enriched in vascular tissues
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additional information
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tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
Q5Z6H1
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
Q6ZAN9
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
brenda
additional information
detailed localization of XET in poplar stems, PttXET16A expression pattern, expression in secondary vascular tissues
brenda
additional information
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isozyme eXET also occurs in other growing regions
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
the same gene encodes a protein, that may be both soluble and bound to the cell wall
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brenda
additional information
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most of the XET activity is strongly associated with the cell wall
brenda
the coding protein of DkXTH1 is targeted to the cell wall by its N-terminal signal peptide
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the coding protein of DkXTH2 is targeted to the cell wall by its N-terminal signal peptide
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transient expression assays in onion epidermal cells indicate direct localization of DkXTH8 to the cell wall via its signal peptide
brenda
the same gene encodes a protein, that may be both soluble and bound to the cell wall
brenda
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cell wall marker enzyme, XET is present in the cell walls in form of a competent glycosyl-enzyme complex
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cell wall marker enzyme, XET is present in the cell walls in form of a competent glycosyl-enzyme complex
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the enzyme is secreted and acts on the cell walls of the host plants
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the enzyme is an integral membrane protein, presence of a trans-membrane domain in 96-SYAILWNMYQIVFYV-110, potential glycosylphosphatidylinositol-anchor (GPI) at position 201 in C-terminal end
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additional information
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expression profiles of members of the XTH gene family, each XTH gene is likely to have a unique fingerprint of expression and regulation
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brenda
additional information
the enzyme contains a signal peptide
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brenda
additional information
the enzyme contains a signal peptide
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brenda
additional information
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prediction of subcellular localization suggests a localization to the secretory pathway
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brenda
additional information
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during interphase EXGT is extensively secreted into the apoplast via the endoplasmic reticulum-Golgi apparatus network, during cytokinesis it is exclusively located in the phragmoblast and eventually transported to the cell plate
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brenda
additional information
the extensibility and xyloglucan endotransglucosylase activity in transgenic tomato plant hypocotyls is increased in the apical zone compared to the basal, overview
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brenda
additional information
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not ionically bound to the cell wall, little XET activity is covalently bound in the cell wall
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brenda
additional information
the putative signal peptide is predicted to be 30 residues long with a cleavage site between Ala30 and Gly31
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brenda
additional information
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the putative signal peptide is predicted to be 30 residues long with a cleavage site between Ala30 and Gly31
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brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
evolution
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the enzyme belongs to the GH16 XTH family, extension clan GH-B enzymes
evolution
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the enzyme belongs to the GH16 XTH family, gene models, overview
evolution
the enzyme belongs to glycosyl hydrolase family 16, which play an important role in endosperm weakening and embryonic expansion during seed germination by loosening the micropylar endosperm and also improving the embryo expansion
evolution
XTHs genes belong to a multi-genetic family, phylogenetic analysis
evolution
XTHs genes belong to a multi-genetic family, phylogenetic analysis
evolution
Petroselinum sp.
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xyloglucan endotransglucosylase (XET) is a homotransglycanase enzyme activity found in all land plants and in some charophytic algae
evolution
xyloglucan endotransglucosylase/hydrolases (XTHs) are encoded in Arabidopsis by a 33-member gene family, isozyme XTH15 is a classical group-I/II XTH
evolution
xyloglucan endotransglucosylase/hydrolases (XTHs) are encoded in Arabidopsis by a 33-member gene family, isozyme XTH31 is a group-III-A XTH
evolution
xyloglucan endotransglycosylase/hydrolase (XTH) enzymes belong to the glycosyl hydrolase family 16 (GH16) superfamily, phylogenetic analysis, gene PrXTH1 belongs to group I of XTHs
evolution
evolutionary relationship of persimmon DkXTH6 and DkXTH7 genes among other plant species and phylogenetic tree, overview. DkXTH6 and DkXTH7 possess several functional domains typical in plant XTHs, including the conserved amino acids (DEIDFEFLG) as a putative active site and together with a potential N-linked glycosylation (N-X-S/T) site
evolution
the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH
evolution
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endoglucanase family 16 members are identified in addition to XTHs of glycoside hydrolase family 16. Phylogenetic analysis clusters the XTHs into three major groups (group I/II, III and ancestral group), group III is subdivided into group IIIA and group IIIB. Similar gene structure and motif number are observed within a group. Two highly conserved domains, glycosyl hydrolase family 16 (GH16-XET) and xyloglucan endotransglycosylase C-terminus (C-XET), are detected by multiple sequences alignment of all XTHs. Segmental replication are detected in the two cultivars, with only the paralogous pair Ac(F153)XTH7-Ac(F153)XTH18 presented in F153 prior to genomic expansion. Genome-wide analysis. Ka/Ks analysis and estimated divergence time of XTHs, detailed phylogenetic analysis and evolutionary relationships, overview
evolution
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endoglucanase family 16 members are identified in addition to XTHs of glycoside hydrolase family 16. Phylogenetic analysis clusters the XTHs into three major groups (group I/II, III and ancestral group), group III is subdivided into group IIIA and group IIIB. Similar gene structure and motif number are observed within a group. Two highly conserved domains, glycosyl hydrolase family 16 (GH16-XET) and xyloglucan endotransglycosylase C-terminus (C-XET), are detected by multiple sequences alignment of all XTHs. Segmental replication are detected in the two cultivars, with only the paralogous pair Ac(F153)XTH7-Ac(F153)XTH18 presented in F153 prior to genomic expansion. Ka/Ks analysis and estimated divergence time of XTHs, detailed phylogenetic analysis and evolutionary relationships, overview
evolution
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phylogenetic analysis of the nucleotide sequences of the open reading frames of the XTHs genes of Arabidopsis thaliana and poplar PnXTH1 shows that this gene is the closest to genes AtXTH5 (endoxyloglucan transferase A4, EXGT-A4) and AtXTH4 (endoxyloglucan transferase A1, EXGT-A1). Identification and analysis of the cis-regulatory elements of the putative promoter region of PnXTH1 gene orthologue from Populus trichocarpa PtXTH1
evolution
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phylogenetic analysis shows that 24 HvXTH genes can be classified into three phylogenetic groups: (I/II, III-A and III-B) and HvXTH15 is in the ancestral group. Xyloglucan endotransglucosylase/hydrolases (XET/XEHs also named XTHs) are a family of xyloglucan modifying enzymes that have two different catalytic activities and can act either as endotransglucosylases (XET, EC 2.4.1.207) or as endohydrolases (XEH, EC 3.2.1.151). XTHs belong to glycoside hydrolase family 16. Each XTH possesses a highly conserved domain (ExDxE) responsible for catalytic activity. Four gene pairs (HvXTH6/7, HvXTH17/18, HvXTH19/20, HvXTH21/22) are detected within 100 kb on chromosome 3H and chromosome 7H, which may be the result of tandem duplication. According to the phylogenetic tree, HvXTH6 and HvXTH7 and HvXTH17/18/19/20 are closely related, which indicates that the gene duplications of these three pair of genes might be related. The active site (ExDxE) containing the residues responsible for catalytic activity is highly conserved in all of the HvXTH family members. The first glutamate residue acts as catalytic nucleophile which initiates the enzymatic reaction and the second one as a base to activate the substrate
evolution
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XTHs are classified together with the lichenases, which hydrolyse mixed-linkage (1->3,1->4)-beta-D-glucans (MLG), in glycoside hydrolase family 16
evolution
xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes. 11 members of the XTH gene family are cloned from Populus tomentosa. A bioinformatics analysis reveals that the 11 PtoXTHs can be classified into three groups. Biochemical analyses of purified PtoXTHs demonstrate that only isozymes PtoXTH27 and PtoXTH34 have detectable xyloglucan endotransglucosylase (XET) activity, while the others do not exhibit XET or XEH activity. PtoXTH34 has a higher affinity for the receptor substrate implying that PtoXTH34 and PtoXTH27 in plants have different substrate structure specificity. Members of the XTH gene family may have one or two enzymatic activities: one is xyloglucan endohydrolase (XEH) activity, which specifically hydrolyzes xyloglucan beta-1,4 glycosidic bonds, and the other is xyloglucan endotransglucosylase (XET) activity, which refers to the transfer of xyloglucan fragments between xyloglucan molecules
evolution
xyloglucan endotransglycosylase/hydrolase (XTH) enzymes hold transglycosylase (XET), hydrolase (XEH), or both activities
malfunction
mutant seedlings defective in XTH15 exhibit no visible growth defects possibly because isozyme XTH16 closely resembles XTH15 in sequence, pI and expression profile
malfunction
overexpression of DkXTH8 results in increased leaf senescence coupled with higher electrolyte leakage in Arabidopsis and faster fruit ripening and softening rates in tomato. Transgenic plants overexpressing DkXTH8 display more irregular and twisted cells due to cell wall restructuring, resulting in wider interstitial spaces with less compact cells. DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants
malfunction
compared to the wild-type enzyme, the single Q108R and K237T, and double-K234T/K237T and triple-H94Q/A104D/Q108R variants exhibit enhanced heterotransglycosylation activities with xyloglucan and (1,4)-beta-D-glucooligosaccharides, while those with (1,3/1,4)- and (1,6)-beta D-glucooligosaccharides are suppressed, the incorporation of xyloglucan to (1,4)-beta-D-glucooligosaccharides by the H94Q variant is influenced most extensively. Physiological effects, overview
malfunction
loss-of-function of XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. Loss-of-function of XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Lower Na+ accumulation in shoot and lower H2O2 content are found in xth30 mutants in response to salt stress. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment. XTH30 affects the xyloglucan oligosaccharide composition during salt stress
malfunction
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the constitutive expression of PnXTH1 in transgenic Nicotiana tabacum promotes an increase in the fresh and dry weight of the aboveground part of the plants mainly due to stimulation of leaf growth. Moreover, the degree of morphological changes in transgenic plants correlate with the expression level of PnXTH1. The root length increases substantially with the action of salt in all transgenic plants
malfunction
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loss-of-function of XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. Loss-of-function of XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Lower Na+ accumulation in shoot and lower H2O2 content are found in xth30 mutants in response to salt stress. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment. XTH30 affects the xyloglucan oligosaccharide composition during salt stress
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metabolism
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enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE)
metabolism
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enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE). XTHs (xyloglucan endotransglucosylases/hydrolases) can catalyse the endolytic cleavage of xyloglucan polymers and the rejoining of the newly generated reducing ends to other xyloglucan molecules, which is referred to as xyloglucan endotransglucosylase (XET) activity. In addition, XTHs can also show xyloglucan endohydrolase (XEH) activity, where water is used as an acceptor, and thus the xyloglucan molecule is hydrolysed
metabolism
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the plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that cut and paste xyloglucan polysaccharides through a transglycosylation process
metabolism
the plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that cut and paste xyloglucan polysaccharides through a transglycosylation process
metabolism
typically, several XET isoforms operate during plant development or in response to environmental stimuli, consequently the expression of XTH genes needs to be regulated
physiological function
XTH28 is specifically involved in the growth of stamen fi laments, and is required for successful automatic self-pollination in certain flowers in Arabidopsis thaliana
physiological function
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xyloglucan endo-transglucosylase can link different polysaccharides in vivo and hence influence cell wall strength, flexibility, and porosity
physiological function
DcXTH2 is associated with petal growth and development during carnation flower opening
physiological function
DcXTH3 is associated with petal growth and development during carnation flower opening
physiological function
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plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases, XTH, that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. The three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, function of GhXTH1 in mediating cotton fiber elongation, the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton, overview
physiological function
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xyloglucan endo-transglycosylases, XETs, encoded by xyloglucan endo-transglycosylases/hydrolase, XTH, genes modify the xyloglucan-cellulose framework of plant cell walls, thereby regulating their expansion and strength. Importance of XET in wood development, xyloglucan dynamics and XTH gene expression in developing wood, overview. Several XTH genes are involved in the development of secondary vascular tissues, XET activity stimulates cell expansion in vessel elements but not in fibers. In young xylem cells, XET activity limits xyloglucan incorporation into the tightly bound wall network but removes it from cell walls in older cells. Importance of XET activity for vascular tissue differentiation and/or function. Increased XET activity has differing effects on the xyloglucan content of expanding and maturing wood cells
physiological function
xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development
physiological function
xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. AtXTH13 might play a role later in root hair development
physiological function
xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. Role for AtXTH12 in root hair initiation. AtXTH13 might play a role later in root hair development
physiological function
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xyloglucan endotransglycosylase/hydrolases, XTH, are believed to play an important role in modifying the cell wall structure through two different, but related actions: the cleavage of a cross-linking xyloglucan polymer, xyloglucan endohydrolase or XEH activity, and transfer of a newly generated end to another sugar polymer, xyloglucan endotransglycosylase, XET, activity
physiological function
xyloglucan xyloglucosyl transferases modulate molecular masses of xyloglucans, size modulations and transfer reactions occur with polymeric acceptor substrates
physiological function
fruit undergoes extensive cell wall disassembly playing a major role in ripening-associated fruit softening. The changes in hemicellulosic polysaccharides are an important feature in the whole softening process. Xyloglucan endotransglycosylase/hydrolase enzymes play a key role in fruit ripening by loosening the cell wall through disassembly of xyloglucan. The ripening of climacteric fruits, such as tomato and apple, is controlled by endogenous ethylene biosynthesis. Ethylene causes a significant increase in activity in the ethylene treated fruits compared with the control fruits, in both tomato and apple fruits, suggesting an increase in the XTH gene expression induced by ethylene
physiological function
fruit undergoes extensive cell wall disassembly playing a major role in ripening-associated fruit softening. The changes in hemicellulosic polysaccharides are an important feature in the whole softening process. Xyloglucan endotransglycosylase/hydrolase enzymes play a key role in fruit ripening by loosening the cell wall through disassembly of xyloglucan. The ripening of climacteric fruits, such as tomato and apple, is controlled by endogenous ethylene biosynthesis. Ethylene causes a significant increase in activity in the ethylene treated fruits compared with the control fruits, in both tomato and apple fruits, suggesting an increase in the XTH gene expression induced by ethylene
physiological function
fruit undergoes extensive cell wall disassembly playing a major role in ripening-associated fruit softening. The changes in hemicellulosic polysaccharides are an important feature in the whole softening process. Xyloglucan endotransglycosylase/hydrolase enzymes play a key role in fruit ripening by loosening the cell wall through disassembly of xyloglucan. t´The ripening of climacteric fruits, such as tomato and apple, is controlled by endogenous ethylene biosynthesis. Ethylene causes a significant increase in activity in the ethylene treated fruits compared with the control fruits, in both tomato and apple fruits, suggesting an increase in the XTH gene expression induced by ethylene
physiological function
gene PrXTH1 shows regulation by ethylene, indicating that it might be a target in the hormonal cascade during the inclination response
physiological function
isozyme XTH15 is predominantly expressed in early-stage organs, and thus seem likely to participate in cell expansion
physiological function
isozyme XTH31 is predominantly expressed in early-stage organs, and thus seem likely to participate in cell expansion. XTH31 is responsible for a specific mode of cell elongation that is particularly Al3+-sensitive. In the wild-type, a second mode of cell expansion that is more Al3+-resistant must be occurring simultaneously
physiological function
the enzyme regulates seed germination in Podophyllum by supplementing its activity along with other endosperm weakening and embryo expansion genes. Increased expression of PhXET influences seed germination in Podophyllum
physiological function
the primary cell wall of flowering plants consists of cellulose fibrils tethered by hemicelluloses (principally xyloglucans) and embedded in an amorphous matrix of pectins and glycoproteins. Plant cell expansion is mainly regulated by cell wall extensibility and results from selective loosening and rearrangement of the load-bearing cellulose/xyloglucan network, at least in flowering plants with type I cell walls where xyloglucan is a predominant hemicellulose. Xyloglucan endotransglucosylase/hydrolases (XTHs), a class of cell wall-modifying proteins, also have the capacity to loosen cell walls. Most XTHs cut and rejoin xyloglucan by xyloglucan endotransglucosylase, EC 2.4.1.207, action, whereas some XTHs hydrolyse xyloglucan by xyloglucan hydrolase, XEH EC 3.2.1.151, action
physiological function
the primary cell wall of flowering plants consists of cellulose fibrils tethered by hemicelluloses (principally xyloglucans) and embedded in an amorphous matrix of pectins and glycoproteins. Plant cell expansion is mainly regulated by cell wall extensibility and results from selective loosening and rearrangement of the load-bearing cellulose/xyloglucan network, at least in flowering plants with type I cell walls where xyloglucan is a predominant hemicellulose. Xyloglucan endotransglucosylase/hydrolases (XTHs), a class of cell wall-modifying proteins, also have the capacity to loosen cell walls. Most XTHs cut and rejoin xyloglucan by xyloglucan endotransglucosylase, EC 2.4.1.207, action, whereas some XTHs hydrolyse xyloglucan by xyloglucan hydrolase, XEH EC 3.2.1.151, action
physiological function
the primary cell wall of flowering plants consists of cellulose fibrils tethered by hemicelluloses (principally xyloglucans) and embedded in an amorphous matrix of pectins and glycoproteins. Plant cell expansion is mainly regulated by cell wall extensibility and results from selective loosening and rearrangement of the load-bearing cellulose/xyloglucan network, at least in flowering plants with type I cell walls where xyloglucan is a predominantchemicellulose. Xyloglucan endotransglucosylase/hydrolases (XTHs), a class of cell wall-modifying proteins, also have the capacity to loosen cell walls. Most XTHs cut and rejoin xyloglucan by xyloglucan endotransglucosylase, EC 2.4.1.207, action, whereas some XTHs hydrolyse xyloglucan by xyloglucan hydrolase, XEH EC 3.2.1.151, action
physiological function
xyloglucan endotransglycosylase/hydrolase enzymes plays a role in the remodeling of cell wall hemicelluloses. Function of isozymes in persimmon fruit development and postharvest softening. DkXTH isozymes function by targeting to the cell wall under regulation of a signal peptide
physiological function
a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
physiological function
fruit softening is mainly associated with cell wall structural modifications, and members of the xyloglucan endotransglucosylase/hydrolase (XTH) family are key enzymes involved in cleaving and rejoining xyloglucan in the cell wall. Isozyme DkXTH8, a xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening
physiological function
isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
physiological function
several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity
physiological function
the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants, e.g. tomato plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. On the other hand, XTH expression is also detected in resistant tomato upon an attack by Cuscuta, which suggests that both host and parasite use these enzymes in their arms race. Cell wall-modifying activities of XTHs during parasitization , overview. XTHs might function to make the host's resources accessible to Cuscuta. One of the defense responses of Solanum lycopersicum is the increased expression of LeXTH1, a gene encoding an XTH. The function of LeXTH1 might be to promote the elongation of tomato epidermal cells that takes place at the site of contact with the parasite. Another possibility is that LeXTH1 is deployed to reinforce the tomato cell walls, presumably as a remedy against the cell wall loosening activity of Cuscuta XTHs at the interface. Increased levels of xyloglucan degradation occur in the haustorium of Cuscuta reflexa and in the infected Solanum lycopersicum host plant
physiological function
-
the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants, e.g. tomato plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. On the other hand, XTH expression is also detected in resistant tomato upon an attack by Cuscuta, which suggests that both host and parasite use these enzymes in their arms race. Cell wall-modifying activities of XTHs during parasitization, overview. XTHs might function to make the host's resources accessible to Cuscuta. At the onset of haustorium development, the swelling of the parasite stem facing the host plant is facilitated by Cuscuta XTHs that promote expansive cell growth through wall loosening. As the haustorium begins its host-invasive growth, XTHs secreted from the infection organ aid tissue penetration by loosening host cell walls. Upon reaching the vascular bundles of its host, the cell wall loosening activity of Cuscuta XTHs at the host-parasite interface enables parasite feeding through apoplastic sugar transfer and/or by promoting vascular tissue differentiation. To prevent exaggerated cell wall loosening under low turgor pressure, Cuscuta XTHs must also strengthen its own walls. When Cuscuta attempts to invade cultivated tomato by deploying wall loosening XTHs at the interface, the counteractive wall strengthening activity of host-encoded XTHs prevents the haustorium from entering the host plant. Model of the putative function of XTHs in the parasitization strategy of Cuscuta, increased levels of xyloglucan degradation occur in the haustorium of Cuscuta reflexa and in the infected Solanum lycopersicum host plant
physiological function
-
the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, e.g. Pelargonium zonale, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. In vivo colocalization of XET activity and donor substrate demonstrate XET activity at the border between host (petioles) and parasite, endotransglucosylation of xyloglucan at the host-parasite interface
physiological function
XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. DkXTH7 is likely to be involved in cell wall assembly, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overvie. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns
physiological function
XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. Enzyme DkXTH6 might take part in cell wall restructuring, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overview. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns
physiological function
xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets
physiological function
xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets
physiological function
xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures
physiological function
enzyme FcXTH2 protein displays mainly xyloglucan hydrolase (XEH) activity and basal xyloglucan endotransglycosylase (XET) activity, and FcXTH2 is primarily a XEH. The enzyme is involved in the modification of the xyloglucans, a type of hemicellulose present in the cell wall. Possible role in strawberry development for enzyme FcXTH2
physiological function
-
FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid
physiological function
-
FvXTH9 and also FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid
physiological function
Populus xyloglucan endotransglycosylase/hydrolase (XTH) plays an important role in the structure and function of plant cell walls and is involved in osmotic stress responses
physiological function
-
roles of HvXTH genes in the growth and development of barley, overview
physiological function
-
the enzyme is involved in regulation of plant growth and stress resistance
physiological function
-
the plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that cut and paste xyloglucan polysaccharides through a transglycosylation process
physiological function
the plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that cut and paste xyloglucan polysaccharides through a transglycosylation process
physiological function
-
transcriptomic analysis indicates that XTHs are involved in the regulation of fruit ripening and crassulacean acid metabolism with tissue specificity, and quantitative real-time PCR expression analysis suggests that Ac(MD2)XTH18 is involved in root growth. XTH proteins perform two diverse catalytic activities: xyloglucan endohydrolase (XEH) and xyloglucan endotransglycosylase (XET)
physiological function
-
transcriptomic analysis indicates that XTHs are involved in the regulation of fruit ripening and crassulacean acid metabolism with tissue specificity. XTH proteins perform two diverse catalytic activities: xyloglucan endohydrolase (XEH) and xyloglucan endotransglycosylase (XET)
physiological function
xyloglucan (XyG) is an important hemicellulose polymer of the primary cell wall in dicotyledons and non-commelinid monocotyledons. XyG plays a vital role in loosening or stiffening the cell wall by binding to cellulose microfibrils with hydrogen bonds during cell elongation. XyG chains can be cleaved or rejoined by xyloglucan endotransglucosylase-hydrolase (XTH). Alteration to the cell wall is one strategy that helps plants adapt to salt stress. Isozyme xyloglucan endotransglucosylase-hydrolase 30 (XTH30) negatively affects salt tolerance in Arabidopsis thaliana. XTH30 modulates XyG side chains, alters abundance of XLFG, cellulose synthesis, and cortical microtubule stability, and negatively affects salt tolerance. And XTH30 aggravates depolymerization of cortical microtubules under salt stress
physiological function
-
plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases, XTH, that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. The three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, function of GhXTH1 in mediating cotton fiber elongation, the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton, overview
-
physiological function
-
xyloglucan (XyG) is an important hemicellulose polymer of the primary cell wall in dicotyledons and non-commelinid monocotyledons. XyG plays a vital role in loosening or stiffening the cell wall by binding to cellulose microfibrils with hydrogen bonds during cell elongation. XyG chains can be cleaved or rejoined by xyloglucan endotransglucosylase-hydrolase (XTH). Alteration to the cell wall is one strategy that helps plants adapt to salt stress. Isozyme xyloglucan endotransglucosylase-hydrolase 30 (XTH30) negatively affects salt tolerance in Arabidopsis thaliana. XTH30 modulates XyG side chains, alters abundance of XLFG, cellulose synthesis, and cortical microtubule stability, and negatively affects salt tolerance. And XTH30 aggravates depolymerization of cortical microtubules under salt stress
-
physiological function
-
the primary cell wall of flowering plants consists of cellulose fibrils tethered by hemicelluloses (principally xyloglucans) and embedded in an amorphous matrix of pectins and glycoproteins. Plant cell expansion is mainly regulated by cell wall extensibility and results from selective loosening and rearrangement of the load-bearing cellulose/xyloglucan network, at least in flowering plants with type I cell walls where xyloglucan is a predominantchemicellulose. Xyloglucan endotransglucosylase/hydrolases (XTHs), a class of cell wall-modifying proteins, also have the capacity to loosen cell walls. Most XTHs cut and rejoin xyloglucan by xyloglucan endotransglucosylase, EC 2.4.1.207, action, whereas some XTHs hydrolyse xyloglucan by xyloglucan hydrolase, XEH EC 3.2.1.151, action
-
physiological function
-
the primary cell wall of flowering plants consists of cellulose fibrils tethered by hemicelluloses (principally xyloglucans) and embedded in an amorphous matrix of pectins and glycoproteins. Plant cell expansion is mainly regulated by cell wall extensibility and results from selective loosening and rearrangement of the load-bearing cellulose/xyloglucan network, at least in flowering plants with type I cell walls where xyloglucan is a predominant hemicellulose. Xyloglucan endotransglucosylase/hydrolases (XTHs), a class of cell wall-modifying proteins, also have the capacity to loosen cell walls. Most XTHs cut and rejoin xyloglucan by xyloglucan endotransglucosylase, EC 2.4.1.207, action, whereas some XTHs hydrolyse xyloglucan by xyloglucan hydrolase, XEH EC 3.2.1.151, action
-
physiological function
-
DcXTH3 is associated with petal growth and development during carnation flower opening
-
physiological function
-
DcXTH2 is associated with petal growth and development during carnation flower opening
-
additional information
-
catalytic nucleophile is Glu85, general acid/base residue is Glu89, Asp87 is the so-called helper residue, molecular dynamics simulations of a branched tetradecasaccharide substrate XXXGXXXG in the active site of xyloglucan endo-transglycosylase. When Asp87 is deprotonated, electrostatic repulsion forces the nucleophilic away from C1 of the sugar ring in subsite 21 and the proton-donating ability of Glu89 iss also weakened due to the formation of a hydrogen bond with Asp87, whereas protonation of Asp87 results in the formation of a hydrogen bond with the catalytic nucleophile and correct positioning of the catalytic machinery
additional information
-
key structural features controlling the activity may involve the loop 1 and 2 regions of the XTH enzymes, homology modeling, detailed overview. The homology models of all 40 poplar XTH proteins is mainly focused on the areas near loop 1 and loop 2
additional information
-
Plants that overexpress GhXTH1 have increased XTH activity and produce mature cotton fibers that are between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions
additional information
-
transgenic up-regulation of XET activity induced changes in cell wall xyloglucan, but its effects are dependent on developmental stage. For instance, XET overexpression increases abundance of the CCRC-M1 epitope in cambial cells and xylem cells in early stages of differentiation but not in mature xylem. Correspondingly, an increase in tightly bound xyloglucan content is observed in primary-walled xylem but a decrease is seen in secondary-walled xylem
additional information
PrXTH1 homology structure modeling using crystal structure of poplar xyloglucan endotransglycosylase Ptt-XET16A, PDB ID 1UMZ
additional information
-
analysis of the xyloglucan endotransglucosylase/hydrolase gene family during apple fruit ripening and softening, overview
additional information
analysis of the xyloglucan endotransglucosylase/hydrolase gene family during apple fruit ripening and softening, overview
additional information
three-dimensional structure modelling of DkXTH1 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
three-dimensional structure modelling of DkXTH1 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
-
three-dimensional structure modelling of DkXTH1 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
three-dimensional structure modelling of DkXTH2 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
three-dimensional structure modelling of DkXTH2 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
-
three-dimensional structure modelling of DkXTH2 based on the template of the crystal structure of PttXET16A (PDB ID 1un1)
additional information
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
additional information
-
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
additional information
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
additional information
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
additional information
docking studies, molecular dynamics simulation and modeling of a three-dimensional structure of FcXTH2 using the structure of TmNXG1 (PDB ID 2UWA) as template. Analysis of the molecular mechanism of interaction of FcXTH2 with xyloglucans as substrate
additional information
-
docking studies, molecular dynamics simulation and modeling of a three-dimensional structure of FcXTH2 using the structure of TmNXG1 (PDB ID 2UWA) as template. Analysis of the molecular mechanism of interaction of FcXTH2 with xyloglucans as substrate
additional information
homology modeling of TmXET6.3 constructed based on the coordinates of the crystal structure of hybrid aspen PttXET16A as the template (PDB ID 1UN1). H94, A104, Q108, K234 and K237 are the key residues that underpinned the acceptor substrate specificity of TmXET6.3, evaluations of TmXET6.3 transglycosylation activities
additional information
-
homology modeling of TmXET6.3 constructed based on the coordinates of the crystal structure of hybrid aspen PttXET16A as the template (PDB ID 1UN1). H94, A104, Q108, K234 and K237 are the key residues that underpinned the acceptor substrate specificity of TmXET6.3, evaluations of TmXET6.3 transglycosylation activities
additional information
structural modeling and analysis of PtoXTH proteins
additional information
structural modeling and analysis of PtoXTH proteins
additional information
-
structural modeling and analysis of PtoXTH proteins
additional information
the model structure of the PrXTH1 is reported, displaying a beta-sandwich structure characteristic of the XTH protein family, with the catalytic residues in the middle of the protein structure, composed of residues Glu79, Asp81, and Glu83
additional information
-
XTHs have a tendency to bind to various surfaces, including chromatography columns and cellulose. Bovine serum albumin (BSA) minimizes binding of solubilized XTHs to tube walls. BSA is the only agent which leads to the measured XET activity being proportional to the concentration of added enzyme: in all three types of plastic, reducing the concentration of the crude enzyme solution from 50% v/v to 15% v/v decrease the measured XET reaction rate by about 70%. Therefore, BSA at 2.5 mg/mL is included in the reaction mixture used in all subsequent XET assays
additional information
-
Plants that overexpress GhXTH1 have increased XTH activity and produce mature cotton fibers that are between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions
-
Show AA Sequence and Transmembrane Helices (8962 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
29500
29600
calculated from amino acid sequence
29700
calculated from amino acid sequence
29900
calculated from amino acid sequence
30100
calculated from amino acid sequence
30200
31000
31737
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
31800
31900
32000
33000 - 35000
SDS-PAGE shows a single, but somewhat diffuse band of 33-35 kDa, indicating that the protein could be glycosylated
33115
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
33400
33780
33790
33810
33850
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man8
34000
-
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE
34020
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man9
34180
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man10
35500
calculated from amino acid sequence
36000
29500
calculated from amino acid sequence
30200
calculated from amino acid sequence
30200
calculated from amino acid sequence
31000
calculated from amino acid sequence
31000
-
x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein
31800
calculated from amino acid sequence
31800
calculated from amino acid sequence
31900
calculated from amino acid sequence
31900
calculated from amino acid sequence
32000
calculated from amino acid sequence
32000
-
x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa
33780
mutant PttXET16-34 E85G, calculated molecular mass
33780
mutant PttXET16-34 E85G, determined by ESI-TOF MS analysis
33790
mutant PttXET16-34 E85A, calculated molecular mass
33790
mutant PttXET16-34 E85A, determined by ESI-TOF MS analysis
33810
mutant PttXET16-34 E85S, calculated molecular mass
33810
mutant PttXET16-34 E85S, determined by ESI-TOF MS analysis
36000
calculated from amino acid sequence
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
?
-
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE
?
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
?
-
x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa
?
-
x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein
?
x * 30000, recombinant unglycosylated His-FLAG-tagged TmXET6.3 lacking the 30-residue signal peptide, SDS-PAGE, x * 31300, recombinant glycosylated His-FLAG-tagged TmXET6.3 lacking the 30-residue signal peptide, SDS-PAGE
additional information
the isozyme sequence contains the conserved DEIDFEFLG motif of XTH
additional information
the isozyme sequence contains the conserved DEIDFEFLG motif of XTH
additional information
three-dimensional structure of isozyme DkXTH1
additional information
three-dimensional structure of isozyme DkXTH2
additional information
analysis of the FcXTH2 model at secondary structure level shows that the enzyme consists of 1 alpha-helix, 2 310-helices and 15 beta-sheets, all of them connected by 20 loops, structure modeling, overview
additional information
-
analysis of the FcXTH2 model at secondary structure level shows that the enzyme consists of 1 alpha-helix, 2 310-helices and 15 beta-sheets, all of them connected by 20 loops, structure modeling, overview
additional information
-
isozymes domain structures, overview. Prediction of the secondary structures of the HvXTH isozyme proteins
additional information
three-dimensional structure analysis of PrXTH1 protein using comparative modeling, the structure shows a beta-sandwich structure with the catalytic site in the middle of the protein structure, composed of residues Glu79, Asp81, and Glu83
additional information
comparison with 10 other XTH isozymes from Populus tomentosa, overview
additional information
comparison with 10 other XTH isozymes from Populus tomentosa, overview
additional information
-
comparison with 10 other XTH isozymes from Populus tomentosa, overview
additional information
in silico and structure prediction analysis, tertiary structure, overview
additional information
-
in silico and structure prediction analysis, tertiary structure, overview
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
no glycoprotein
proteolytic modification
additional information
glycoprotein
-
AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity
glycoprotein
all 4 isoenzymes, TCH4, Meri-5, EXGT and XTR9, are N-glycosylated, requirement of glycosylation for activity of the isoenzymes differs
glycoprotein
AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site
glycoprotein
one N-glycosylation site situated close to the predicted catalytic residues, enzyme is a high-mannose-type glycan, glycosylation is required for activity, deglycosylation results in 40% loss of activity, overview
glycoprotein
the isozyme sequence contains a potential N-linked glycosylation signal site
glycoprotein
the enzyme contains a potential N-linked glycosylation (N-X-S/T) site
glycoprotein
the enzyme contains a potential N-linked glycosylation (N-X-S/T) site
glycoprotein
FcXTH2 shows an N-glycosylation site that is located far to the active site and oriented on the edge of the opposite face. In the primary sequence of the protein, the N-glycosylation site is spaced by 17 residues from the catalytic triad
glycoprotein
-
all HvXTH protein members, except HvXTH15, have a conserved N-glycosylation site. The N-glycosylation site domain (NxT/S/Y) is thought to play a role in protein stability
glycoprotein
two putative glycosylation sites at amino acid positions 14 and 114
glycoprotein
isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif
glycoprotein
isozyme XTH1, not isozyme XTH21, possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif
glycoprotein
PttXET16A contains a putative N-glycosylation site
glycoprotein
enzyme contains a conserved N-glycosylation site situated proximal to the predicted catalytic site, deglycosylation by endoglycosidase H or by site-directed mutagenesis does not influence the enzyme activity
glycoprotein
enzyme are deglycosylated with commercial N-glycosidase F. Unglycosylated TmXET6.3 due to N-deglycosylation loses its enzyme activity, it is likely that N-glycosylation affects protein folding and/or catalytic activity
no glycoprotein
isozymes XTH19 and XTH20
proteolytic modification
-
21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein
proteolytic modification
-
mature TCH4 polypeptide lacks the putative N-terminal signal sequence
proteolytic modification
contains a hydrophobic N-terminus indicating a signal peptide
proteolytic modification
PttXET16A has a putative 22 amino acids signal peptide at the N-terminus
proteolytic modification
-
tXET-B1 and tXET-B2 contain a hydrophobic signal peptide of 20 and 18 amino acids, respectively, typical of an exported protein
proteolytic modification
-
hydrophobic core of 24 amino acids at the N-terminus, characteristic of a signal peptide, enzyme must be transported through the cell membrane in order to reach the cell wall, DNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein
additional information
-
co- and post-translational eucaryotic-specific modifications of TCH4 are critical for optimal XET activity
additional information
co- and post-translational eucaryotic-specific modifications of TCH4 are critical for optimal XET activity
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling of structure suggests that key amino acid substitutions Trp-Pro, Gly-Ser and Arg-Leu are responsible for the evolution of HTGs specificity from the xyloglucan-acting homo-transglycanases
purified recombinant enzyme, free enzyme or in complex with a xyloglucan nonasaccharide XLLG, hanging drop vapour diffusion method, 20°C, 0.002 ml of 10 mg/ml protein in 10 mM HEPES, pH 7.0, mixed with equal volume of a solution containing 1.0 M NaOAc and 0.2 M imidazole, and again mixed with equal volume of reservoir solution containing 0.4 M potassium sodium tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, overall structure is a beta-sandwich, molecular modeling
active-site loop deletion variant NXG1-DELTAYNIIG in free and product-complexed forms, using 0.2 M sodium formate, 0.1 M Bis Tris propane buffer at pH 6.5, and 20% (w/v) PEG 3350
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E97Q
-
At-XTH22: a Glu to Gln substitution, converting the active site sequence DEIDFEFL to DQIDFEFL, abolishes XET activity
E85A
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
E85G
E85S
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
N93S
site-directed mutagenesis, activity and thermal stability of the mutant enzyme are similar to the wild-type enzyme, but the mutant shows reduced solubility
H94Q
site-directed mutagenesis, the mutant enzymes exhibits increased activity with cello-oligosacchrides, relative to those with the natural xyloglucan octasaccharide acceptor substrate
H94Q/A104D/Q108R
site-directed mutagenesis, the mutant enzymes exhibits increased activity with cello-oligosacchrides, relative to those with the natural xyloglucan octasaccharide acceptor substrate
K234T/K237T
site-directed mutagenesis, the mutant enzymes exhibits increased activity with cello-oligosacchrides, relative to those with the natural xyloglucan octasaccharide acceptor substrate
K237T
site-directed mutagenesis, the mutant enzymes exhibits increased activity with cello-oligosacchrides, relative to those with the natural xyloglucan octasaccharide acceptor substrate
Q108R
site-directed mutagenesis, the mutant enzymes exhibits increased activity with cello-oligosacchrides, relative to those with the natural xyloglucan octasaccharide acceptor substrate
additional information
E85G
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
additional information
overexpression of gene AtXTH18 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH18 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH18 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
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overexpression of gene AtXTH18 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH19 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH19 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH19 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
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overexpression of gene AtXTH19 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH20 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH20 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
overexpression of gene AtXTH20 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
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overexpression of gene AtXTH20 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
additional information
generation of loss-of-function of XTH30 mutants, xth30 mutants xth30-1 and xth30-2, phenotype, overview. Homozygous xth30 mutants are identified by T-DNA insertion-based PCR. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment
additional information
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generation of loss-of-function of XTH30 mutants, xth30 mutants xth30-1 and xth30-2, phenotype, overview. Homozygous xth30 mutants are identified by T-DNA insertion-based PCR. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment
additional information
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generation of loss-of-function of XTH30 mutants, xth30 mutants xth30-1 and xth30-2, phenotype, overview. Homozygous xth30 mutants are identified by T-DNA insertion-based PCR. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment
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additional information
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overexpression of gene AtXTH20 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
-
additional information
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overexpression of gene AtXTH19 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
-
additional information
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overexpression of gene AtXTH18 in Arabidopsis thaliana stimulates growth of hypocotyls with more linear creep, higher values of initial deformation stress, cell wall extensibility and in vitro cell wall yield compared to the wild-type, phenotype, overview
-
additional information
recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum, DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants
additional information
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Fragaria x ananassa cv. Elsanta fruits infiltrated with FvXTH9 and FvXTH6 ripen faster and show decreased firmness compared with the empty vector control pBI121
additional information
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creation of transgenic Nicotiana tabacum plants with constitutive expression of the PnXTH1 gene (the orthologue of PtrXTH1 from the black poplar, Populus nigra). Transgenic tobacco plants are characterized by an increase in leaf size and fresh and dry weight of the above ground part under normal growth conditions. When grown under conditions of salinization and drought, transgenic plants show increased stress resistance due to the maintenance of cell expansion in roots and stems at a higher level and the ability to more effectively retain water in leaves compared with wild-type plants. Molecular and morphological analysis of transgenic tobacco plants, phenotype, overview. The constitutive expression of PnXTH1 promotes an increase in the fresh and dry weight of the aboveground part of the plants mainly due to stimulation of leaf growth. Moreover, the degree of morphological changes in transgenic plants correlate with the expression level of PnXTH1. The root length increases substantially with the action of salt in all transgenic plants
additional information
overexpression of gene XET16A in Arabidopsis thaliana does not stimulate growth of hypocotyls, phenotype, overview
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 7
-
the enzyme is 100% stable for 24 h in the pH range 5-7 at room temperature, but at pH values below and above this range 50-75% of the activity is lost
674988
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0 - 37
-
isozyme XTH14 shows high activity at 37°C, and at least 80% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 60% of ist activity at 37°C. The activity abruptly decreases to about 30% at 0°C. Isozyme XTH26 shows high activity at 37°C, and at least 50% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 30% of ist activity at 37°C, while the activity abruptly decreases to about 15% at 0°C
38 - 50
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the activity of XETs from nasturtium seeds is stable at 40°C during 2 h (at 38°C during 8 h), but it is completely lost at 50°C
44
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TCH4 protein: undetectable activity, more heat-sensitive than the total XET activity
5
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5°C
52.3
unfolding temperature for the recombinant N93S mutant enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M
52.9
unfolding temperature for the recombinant wild-type enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M
95
about 25% of isozyme XET6 activity survives incubation at 95°C for 1 min
additional information
additional information
-
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant
additional information
-
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
about 30% activity is lost during one freeze-thaw cycle
activity is lost after partial purification and desalting, ascorbic acid and 2-mercaptoethanol cannot prevent the loss, activity can be restored by addition of a wide variety of inorganic and organic salts
-
addition of bovine serum albumin does not affect enzyme activity or stability
-
progressive purification destabilizes XET activity, 10% glycerol stabilizes
repeated cycles of freezing and thawing reduce its activity to around 10%
progressive purification destabilizes XET activity, 10% glycerol stabilizes
-
progressive purification destabilizes XET activity, 10% glycerol stabilizes
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Glycerol
-
the addition of 10% (v/v) glycerol has no affect on XET activity after several freeze-thawing cycles
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 100 mM succinate or ammonium acetate buffers at pH 6.0 containing 5 mM calcium chloride, 1 year, no loss of activity
-
14°C, 0.1 mg/ml enzyme in 50 mM ammonium acetate, pH 5.5, with 0.5 M ammonium sulfate, complete loss of activity within 3 months
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Hi-Trap SP Sepharose column chromatography, Superdex 75 gel filtration
-
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography, chromatofocusing on PBE-94, and Bio-Gel P-60 gel filtration
-
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
His-Trap chelating column chromatography
isozymes sXET and eXET from germinated seeds, i.e. cotyledons, and from epicotyls, respectively, to near homogeneity by ammonium sulfate fractionation, anion exchange chromatography, adsorption and desorption of the enzyme-substrate complex on cellulose, and hydrophobic-interaction chromatography
-
native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification
partial, recombinant TCH4, Meri-5, EXGT and XTR9, expressed in Sf9 insect cells
polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration
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proteins are extracted from tomato plants
recombinant AtXTH12 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH13 partially from Pichia pastoris by ammonium sulfate fractionation, AtXTH13 is purified to apparent homogeneity by xylogluco-oligosaccharide affinity chromatography
recombinant AtXTH17 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH18 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH19 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant enzyme from Pichia pastoris by strong cation exchange chromatography and gel filtration in a sequential combination
recombinant enzyme XTH2 12.43fold from Pichia pastoris by anion exchange chromatography, cation exchange chromatography, desalting gel filtration, and affinity chromatography
recombinant extracellular His-/FLAG-tagged wild-type and mutant enzymes expressed from Pichia pastoris strain GS115 by nickel affinity chromatography and desalting gel filtration
recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography
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recombinant His-tagged isozyme DkXTH3 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme DkXTH4 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme DkXTH5 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme without the N-terminal leader sequence from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged mature coding region of isozyme DkXTH1 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged XTH1 from Pichia pastoris by anion exchange chromatography and nickel affinity chromatography
recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3)
recombinant wild-type and mutant enzymes from Pichia pastoris by ultrafiltration, one or two steps of cation exchange chromatography, dialysis, and adsorption chromatography
Sepharose Q column chromatography, Phenyl-Sepharose chromatography, PBE-94 column chromatography and Bio-Gel P-60 gel filtration
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SP-Sepharose fast flow column chromatography and S-source 15S gel filtration
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general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
-
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
-
His-Trap chelating column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH27 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH34 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants
24 XTH genes, HvXTH1-24, sequence comparisons and phylogenetic analysis, the 24 genes are unevenly distributed on the 7 chromosomes, with 10 of them specifically located on chromosome 7H, genetic organization, sequence comparisons and phylogenetic analysis, overview. Four gene pairs (HvXTH6/7, HvXTH17/18, HvXTH19/20, HvXTH21/22) are detected within 100 kb on chromosome 3H and chromosome 7H, which may be the result of tandem duplication
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6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6
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AtXTH12, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH13, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115. AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site
AtXTH17, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH18, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH19, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
cloning and expression of TCH4, Meri-5, EXGT and XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced
cloning and expression of XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity
DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment
DNA and amino acid seuence determination and analysis, the poplar genome contains 40 XTH genes, overview
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DNA sequence determination and analysis, functional expression in Pichia pastoris
DNA sequence determination and analysis, methanol-induced, functional expression of wild-type and N93S mutant enzymes in Pichia pastoris, the recombinant wild-type enzyme is heterogeneous due to presence of variable N-glycosylation and incomplete cleavage of the alpha-factor secretion signal peptide
expressed in Arabidopsis thaliana
expressed in Pichia pastoris
expressed in Pichia pastoris strain GS115
expression in Solanum lycopersicum cv. Money Maker via transfection by Agrobacterium tumefaciens strain C58C1:pGV2260 under control of promoter CaMV-35S. Expression analysis of SlXTH1 tomato gene by real-time quantitative PCR from apical and basal hypocotyls segments of wild-type and transgenic tomato lines, and extensibility of cell walls from apical and basal hypocotyls segments of wild type and transgenic tomato lines
gene AtXTH18, expression analysis by real-time semiquantitative RT-PCR, overexpression in Arabidopsis thaliana
gene AtXTH19, expression analysis by real-time semiquantitative RT-PCR, overexpression in Arabidopsis thaliana
gene AtXTH20, expression analysis by real-time semi-quantitative RT-PCR, overexpression in Arabidopsis thaliana
gene DcXTH1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
gene DcXTH2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissue expression analysis by real-time RT-PCR
gene DcXTH3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
gene DkXTH1, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, mature coding region of DkXTH1 is expressed in Escherichia coli strain BL21, recombinant expression of GFP-tagged isozyme lacking the sugnal peptide and of the DkXTH1 signal peptide fused to GFP in onion cells
gene DkXTH2, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, the isozyme is expressed without the N-terminal leader sequence in Escherichia coli strain BL21, recombinant expression of full-length GFP-tagged isozyme in onion cells
gene DkXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DkXTH4, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DkXTH5, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative realtime PCR expression analysis. The isozyme encoding DKXTH1 and DKXTH2 genes show different expression patterns during fruit ripening
gene DXTH2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative realtime PCR expression analysis. The isozyme encoding DKXTH1 and DKXTH2 genes show different expression patterns during fruit ripening
gene HvXET3, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene HvXET4, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene HvXET6, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene LeXET2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH10, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH11, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene MdXTH9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene PrXTH1, DNA and amino acid sequence determination and analyyis, phylogenetic analysis, gene PrXTH1 belongs to group I of XTHs. The recombinant purified protein has only transglycosylase activity. Quantitative real time-PCR enzyme expression analysis. Recombinant expression of C-terminally HIs-tagged enzyme in Pichia pastoris strain X-33
gene SIXTH12, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXET4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene SlXTH9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene structure, genomic localization and phylogenetic relationship of the XTH gene family
-
gene tXET-B1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene tXET-B2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene XET02, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
gene XET1, DNA and amino acid sequence determination and analysis, cloning from germinating seeds, sequence comparisons and phylogenetic tree
gene XET16A, expression analysis by real-time semi-quantitative RT-PCR, overexpression in Arabidopsis thaliana
gene xet6.3, sequence comparisons and analysis, recombinant expression of His-/FLAG-tagged wild-type and mutant enzymes in Escherichia coli strain XL10-Gold, recombinant expression of His-/FLAG-tagged wild-type and mutant enzymes in Pichia pastoris strain GS115, from the latter the enzymes are secreted
gene XTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, constitutive recombinant expression in Nicotiana tabacum plants via transfection with Agrobacterium tumefaciens strain AGL0
-
gene XTH1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis
-
gene XTH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene XTH1, recombinant expression of His-tagged enzyme in Pichia pastoris
gene XTH10, isolation of genes XTH2 and XTH10 from an SSH library for the Taishanzaoxia apple, recombinant expression in Solanum lycopersicum plants via Agrobacterium tumefaciens strain LBA4404 transfection method
gene XTH15, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted
gene XTH2, cloned from young leaves, recombinant expression in Pichia pastoris strain X-33, subcloning in Escherichia coli
gene XTH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene XTH2, isolation of genes XTH2 and XTH10 from an SSH library for the Taishanzaoxia apple, recombinant expression in Solanum lycopersicum plants via Agrobacterium tumefaciens strain LBA4404 transfection method
gene XTH30, recombinant expression of GFP- or YFP-tagged isozyme XTH30 in Arabidopsis thaliana and heterologous expression of wild-type and mutant enzymes in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation, real-time PCR enzyme expression analysis. Expression of a XTH30 promoter::GUS construct in Arabidopsis thaliana
gene XTH31, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted
gene XTH6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells. Recombinant expression of DkXTH6 without the signal peptide in Escherichia coli strain BL21
gene XTH7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of DkXTH7 without the signal peptide in Escherichia coli strain BL21
gene XTH8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression in onion epidermal cells at the cell wall, recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum
genes GhXTH1, GhXTH2, and GhXTH3, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, recombinant overexpression of isozyme GHXTH1 in transgenic cotton plants using Agrobacterium tumefaciens transfection method under transcriptional control of the CaMV 35S promoter
-
genes SIXTH13-SlXTH25, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
genes XET1 and NXG1 encode 2 enzyme forms, XET1 cDNA is cloned and sequenced, amino acid sequence of NGX1
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identification of several genes designated Ac(F153)XTH1 to Ac(F153)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview
-
identification of several genes designated Ac(MD2)XTH1 to Ac(MD2)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview
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into the pBIm and pCAM-BIA1300 vector, and into the pPIC6A expression vector for the production of recombinant AtXTH21 protein using the Pichia pastoris expression system
into the pET28a+ vector for transformation of Escherichia coli BL21 cells, into the pPICZalphaA vector for transformation of Pichia pastoris X-33 cells, into the pCAMBIA1302 and pCAMBIA1300 vectors for transformation of onion and Arabidopsis thaliana cells, respectively
into the pMD-18T vector
isoform XTH5 is expressed in Escherichia coli BL21 cells
isoform XTH7 is expressed in Escherichia coli BL21 cells
NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan
phylogenetic tree and expression analysis of XTH genes, transgenic overexpression of PtxtXET16-34 in Populus tremula x tremuloides
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phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH6 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH9, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells
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phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum via Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells
-
PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence
single copy gene, cDNA encoding XET is cloned, sequenced and encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein, NXG1 and 2 may be different alleles of the XET gene
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small multi-gene family encodes XET: tXETB1, -B2, -B3 and -B4, tXET-B2 is cloned, sequenced and overexpressed in Escherichia coli, nucleotide and amino acid sequence of tXET-B1
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XTH gene DcXTH4, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ethylene transiently increases the isozyme expression in fruits, overview
expression of DkXTH8 is stimulated by propylene and abscisic acid during storage at room temperature
expression of DkXTH8 is suppressed by gibberellic acid and 1-methylcyclopropene during storage at room temperature
expression of XTH30, encoding xyloglucan endotransglucosylase-hydrolase 30, is strongly upregulated in response to salt stress in Arabidopsis thaliana. Salt stress decreases the relative level of XXFG and XXFG+Ac, but increases the level of XXXG, XLFG, and XLFG+Ac in the wild-type. More importantly, the increase of XLFG caused by salt stress in the wild-type is partly blocked in xth30 mutants. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves
in young aspen leaves, the expression of PtrXTH1 is induced by cytokinins, auxins, and brassinosteroids. The content of PtrXTH1 transcripts increases under the constitutive expression of the PnARGOS-LIKE (UniProt ID I6SWL6) gene. Participation of the PnARGOS-LIKE gene in the regulation of PtrXTH1 gene expression
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isozyme XTH7 is expressed at the red ripe stage
no growth phenotype difference is observed between xth30 mutants and wild-type under heavy metal stresses (CdCl2 and ZnSO4) or the plant hormone abscisic acid (ABA) treatment
reduction of fruit firmness and ethylene production in cv. Taishanzaoxia apple treated with 1-methylcyclopropene (1-MCP), comparison to effects on expression of other XET isozymes in apple, overview. Fruit softening is delayed in fruits treated with 1-MCP and XTH2, XTH7, XTH10, and XTH11 expression levels are suppressed 0-2 days after harvest (DAH), but are upregulated 2-5 DAH accompanied by the accumulation of ethylene
reduction of fruit firmness and ethylene production in cv. Taishanzaoxia apple treated with 1-methylcyclopropene, comparison to effects on expression of other XET isozymes in apple, overview. Fruit softening is delayed in fruits treated with 1-MCP and XTH2, XTH7, XTH10, and XTH11 expression levels are suppressed 0-2 days after harvest (DAH), but are upregulated 2-5 DAH accompanied by the accumulation of ethylene
the expression levels of isozyme DkXTH1 in Fuping Jianshi persimmon fruit is markedly induced by cold treatment, and it is also higher in the firmer persimmon cultivar Ganmaokui compared with those in the softer cultivar (Fuping Jianshi) during storage at 25°C
the expression of isozyme XTH5 is clearly associated with fruit ripening
the expression of isozyme XTH8 is clearly associated with fruit ripening
XTH expression responds to phytohormone treatments, treatments with brassinoids, gibberellic acid, and, naphthalene acetic acid all increase the expression of GhXTH1 transcripts in 15 DPA fibers, overview
XTH1 is expressed at the red ripe stage
XTH12 is expressed at the red ripe stage
XTH2 is expressed at the red ripe stage
XTH3 is expressed at the red ripe stage
XTH4 is expressed at the red ripe stage
XTH6 is expressed at the red ripe stage
XTH9 is expressed at the red ripe stage
ethylene transiently increases the isozyme expression in fruits, overview
ethylene transiently increases the isozyme expression in fruits, overview
expression of XTH30, encoding xyloglucan endotransglucosylase-hydrolase 30, is strongly upregulated in response to salt stress in Arabidopsis thaliana. Salt stress decreases the relative level of XXFG and XXFG+Ac, but increases the level of XXXG, XLFG, and XLFG+Ac in the wild-type. More importantly, the increase of XLFG caused by salt stress in the wild-type is partly blocked in xth30 mutants. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves
expression of XTH30, encoding xyloglucan endotransglucosylase-hydrolase 30, is strongly upregulated in response to salt stress in Arabidopsis thaliana. Salt stress decreases the relative level of XXFG and XXFG+Ac, but increases the level of XXXG, XLFG, and XLFG+Ac in the wild-type. More importantly, the increase of XLFG caused by salt stress in the wild-type is partly blocked in xth30 mutants. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves
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no growth phenotype difference is observed between xth30 mutants and wild-type under heavy metal stresses (CdCl2 and ZnSO4) or the plant hormone abscisic acid (ABA) treatment
no growth phenotype difference is observed between xth30 mutants and wild-type under heavy metal stresses (CdCl2 and ZnSO4) or the plant hormone abscisic acid (ABA) treatment
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XTH expression responds to phytohormone treatments, treatments with brassinoids, gibberellic acid, and, naphthalene acetic acid all increase the expression of GhXTH1 transcripts in 15 DPA fibers, overview
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XTH expression responds to phytohormone treatments, treatments with brassinoids, gibberellic acid, and, naphthalene acetic acid all increase the expression of GhXTH1 transcripts in 15 DPA fibers, overview
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EXTERNAL LINKS (specific for EC-Number 2.4.1.207)
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Release 2023.1 (January 2023)
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