Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, functional expression in Pichia pastoris | Populus tremula x Populus tremuloides |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, free enzyme or in complex with a xyloglucan nonasaccharide XLLG, hanging drop vapour diffusion method, 20°C, 0.002 ml of 10 mg/ml protein in 10 mM HEPES, pH 7.0, mixed with equal volume of a solution containing 1.0 M NaOAc and 0.2 M imidazole, and again mixed with equal volume of reservoir solution containing 0.4 M potassium sodium tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, overall structure is a beta-sandwich, molecular modeling | Populus tremula x Populus tremuloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Populus tremula x Populus tremuloides | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Populus tremula x Populus tremuloides | key enzyme in all plant processes that require cell wall remodeling | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | Q8GZD5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris by strong cation exchange chromatography and gel filtration in a sequential combination | Populus tremula x Populus tremuloides |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | substrate binding and catalytic mechanism, active site structure, structure-function study | Populus tremula x Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | key enzyme in all plant processes that require cell wall remodeling | Populus tremula x Populus tremuloides | ? | - |
? | |
additional information | transglycosylation acceptor binding mechanism, structure-function study | Populus tremula x Populus tremuloides | ? | - |
? | |
xyloglucan nonasaccharide + xyloglucan | nonasaccharide XLLG, a 2-chloro-4-nitrophenyl-nonasaccharide is no substrate, tamarind xyloglucan | Populus tremula x Populus tremuloides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16 | Populus tremula x Populus tremuloides |
XET16A | - |
Populus tremula x Populus tremuloides |
xyloglucan endotransglucosylase 16A | - |
Populus tremula x Populus tremuloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Populus tremula x Populus tremuloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Populus tremula x Populus tremuloides |