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Literature summary for 2.4.1.181 extracted from

  • Khouri, H.E.; Ibrahim, R.K.
    Purification and some properties of five anthraquinone-specific glucosyltransferases from Cinchona succirubra cell suspension culture (1987), Phytochemistry, 26, 2531-2535.
No PubMed abstract available
Search for more literature for 2.4.1.181

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 63% activation at 10 mM Cinchona succirubra
EDTA 30% activation at 1 mM Cinchona succirubra

General Stability

General Stability Organism
in absence of SH-group protectors, 50% loss of activity after 48 h, in presence of 14 mM 2-mercaptoethanol, 50% loss of activity after 2 weeks Cinchona succirubra

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 60% inhibition at 1 mM Cinchona succirubra
Cu2+ 50% inhibition at 1 mM Cinchona succirubra
iodoacetamide 60% inhibition at 1 mM Cinchona succirubra
iodoacetate 30% inhibition at 1 mM Cinchona succirubra
Mn2+ 45% inhibition at 10 mM Cinchona succirubra
N-ethylmaleimide 92% inhibition at 1 mM Cinchona succirubra
p-chloromercuribenzoate 47% inhibition at 1 mM, 10 mM dithiothreitol protects Cinchona succirubra
phenylmercuric acetate 80% inhibition at 1 mM, 10 mM dithiothreitol protects Cinchona succirubra
Zn2+ 77% inhibition at 1 mM Cinchona succirubra

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 emodin isoform I Cinchona succirubra
0.01
-
 anthrapurpurin isoform II Cinchona succirubra
0.01
-
 quinizarin isoform III Cinchona succirubra
0.01
-
 2,6-dihydroxyanthracene-9,10-dione isoform IV Cinchona succirubra
0.01
-
 1,8-dihydroxyanthraquinone isoform V Cinchona succirubra

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no cation requirement Cinchona succirubra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Cinchona succirubra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + a hydroxyanthraquinone Cinchona succirubra a range of anthraquinones and some flavones can act as acceptors UDP + a glucosyloxyanthraquinone
-
 ?

Organism

Organism UniProt Comment Textmining
Cinchona succirubra
-
 5 distinct glucosylating activities with different pI values and substrate specificities, named isoforms I-V
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cinchona succirubra

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Cinchona succirubra
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cinchona succirubra

Storage Stability

Storage Stability Organism
-20°C, 25 mM histidine/HCl buffer, pH 7.0, 10% glycerol, 10 mM dithioerythritol, stable for 2 months Cinchona succirubra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 1,8-dihydroxyanthraquinone
-
 Cinchona succirubra UDP + 1-O-beta-D-glucopyranosyl-1,8-dihydroxyanthraquinone
-
 ?
UDP-alpha-D-glucose + 2,6-dihydroxyanthracene-9,10-dione
-
 Cinchona succirubra UDP + 6-hydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + anthrapurpurin
-
 Cinchona succirubra UDP + 2,7-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + emodin
-
 Cinchona succirubra UDP + 7,8-dihydroxy-3-methyl-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + quinizarin
-
 Cinchona succirubra UDP + 4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
-
 ?
UDP-glucose + a hydroxyanthraquinone a range of anthraquinones and some flavones can act as acceptors Cinchona succirubra UDP + a glucosyloxyanthraquinone
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cinchona succirubra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 for isoforms I-V Cinchona succirubra