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IUBMB Comments A range of anthraquinones and some flavones can act as acceptors; best substrates are emodin, anthrapurpurin, quinizarin, 2,6-dihydroanthraquinone and 1,8-dihydroxyanthraquinone.
Synonyms
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anthraquinone-specific glucosyltransferase
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uridine diphosphoglucose-anthraquinone glucosyltransferase
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UDP-glucose + an hydroxyanthraquinone = UDP + a glucosyloxyanthraquinone
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hexosyl group transfer
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UDP-glucose:hydroxyanthraquinone O-glucosyltransferase
A range of anthraquinones and some flavones can act as acceptors; best substrates are emodin, anthrapurpurin, quinizarin, 2,6-dihydroanthraquinone and 1,8-dihydroxyanthraquinone.
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UDP-alpha-D-glucose + 1,8-dihydroxyanthraquinone
UDP + 1-O-beta-D-glucopyranosyl-1,8-dihydroxyanthraquinone
Cinchona succirubra
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Substrates: -
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UDP-alpha-D-glucose + 2,6-dihydroxyanthracene-9,10-dione
UDP + 6-hydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl beta-D-glucopyranoside
Cinchona succirubra
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Substrates: -
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UDP-alpha-D-glucose + anthrapurpurin
UDP + 2,7-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
Cinchona succirubra
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Substrates: -
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UDP-alpha-D-glucose + emodin
UDP + 7,8-dihydroxy-3-methyl-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
Cinchona succirubra
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Substrates: -
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UDP-alpha-D-glucose + quinizarin
UDP + 4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
Cinchona succirubra
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Substrates: -
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UDP-glucose + a hydroxyanthraquinone
UDP + a glucosyloxyanthraquinone
Cinchona succirubra
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Substrates: a range of anthraquinones and some flavones can act as acceptors
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UDP-glucose + a hydroxyanthraquinone
UDP + a glucosyloxyanthraquinone
Cinchona succirubra
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Substrates: a range of anthraquinones and some flavones can act as acceptors
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additional information
Cinchona succirubra
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no cation requirement
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Co2+
Cinchona succirubra
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60% inhibition at 1 mM
Cu2+
Cinchona succirubra
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50% inhibition at 1 mM
iodoacetamide
Cinchona succirubra
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60% inhibition at 1 mM
iodoacetate
Cinchona succirubra
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30% inhibition at 1 mM
Mn2+
Cinchona succirubra
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45% inhibition at 10 mM
N-ethylmaleimide
Cinchona succirubra
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92% inhibition at 1 mM
p-chloromercuribenzoate
Cinchona succirubra
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47% inhibition at 1 mM, 10 mM dithiothreitol protects
phenylmercuric acetate
Cinchona succirubra
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80% inhibition at 1 mM, 10 mM dithiothreitol protects
Zn2+
Cinchona succirubra
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77% inhibition at 1 mM
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dithiothreitol
Cinchona succirubra
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63% activation at 10 mM
EDTA
Cinchona succirubra
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30% activation at 1 mM
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0.01
1,8-dihydroxyanthraquinone
Cinchona succirubra
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isoform V
0.01
2,6-dihydroxyanthracene-9,10-dione
Cinchona succirubra
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isoform IV
0.01
anthrapurpurin
Cinchona succirubra
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isoform II
0.01
emodin
Cinchona succirubra
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isoform I
0.01
quinizarin
Cinchona succirubra
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isoform III
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additional information
Cinchona succirubra
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7
Cinchona succirubra
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for isoforms I-V
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30
Cinchona succirubra
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assay at
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Cinchona succirubra
5 distinct glucosylating activities with different pI values and substrate specificities, named isoforms I-V
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brenda
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Cinchona succirubra
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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50000
Cinchona succirubra
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gel filtration
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in absence of SH-group protectors, 50% loss of activity after 48 h, in presence of 14 mM 2-mercaptoethanol, 50% loss of activity after 2 weeks
Cinchona succirubra
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-20°C, 25 mM histidine/HCl buffer, pH 7.0, 10% glycerol, 10 mM dithioerythritol, stable for 2 months
Cinchona succirubra
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Khouri, H.E.; Ibrahim, R.K.
Purification and some properties of five anthraquinone-specific glucosyltransferases from Cinchona succirubra cell suspension culture
Phytochemistry
26
2531-2535
1987
Cinchona succirubra
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brenda
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