Cloned (Comment) | Organism |
---|---|
since chitin synthase 2 can not expressed in bacterial cells or insect cells in an active form, Saccharomyces cerevisiae is used as the host for overexpression | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Nikkomycin | nikkomycin Z | Saccharomyces cerevisiae | |
nikkomycin Z | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | significant chitin synthase activity in the presence of Mg2+ or Mn2+, even without trypsin treatment | Saccharomyces cerevisiae | |
Mn2+ | significant chitin synthase activity in the presence of Mg2+ or Mn2+, even without trypsin treatment | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
chitin synthetase 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | chitin synthase 2 activity is independent of the N-terminal 193 amino acid truncation, because partially purified full length enzyme also exhibits the activity without trypsin treatment in the presence of appropriate cations | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | - |
Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
nikkomycin Z | - |
Saccharomyces cerevisiae |