Cloned (Comment) | Organism |
---|---|
construction of a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system, and functional expression of His-FLAG-tagged human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region. The enzyme is secreted. The enzyme expressed in silkworm is glycosylated, the structure of N-glycans in the recombinant hGnTII is suggested to be of the complex type, pauci-mannosidic-type glycans, and the removal of the glycans does not affect the enzymatic activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme mutant HF-hGnTIIDELTA29 lacking the N-terminal cytosolic tail and transmembrane region | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | GnTII is a Golgi-localized type II transmembrane enzyme | Homo sapiens | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q10469 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | analysis of N-glycan attached to HF-hGnTIIDELTA29 mutant enzyme recombinantly expressed in silkworm using Endoglycosidase H (Endo H) and peptide:N-glycanase F (PNGase F) | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-FLAG-tagged hGnTII from silkworm larval hemolymph in tandem affinity purification and ultrafiltration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme exhibits glycosyltransferase activity and strict substrate specificity. The acceptor substrate specificity of enzyme mutant HF-hGnTIIDELTA29 is examined using UDP-GlcNAc as a donor substrate and MM-PA, GnM-PA, GnGn-PA, and p-nitrophenyl-alpha-mannopyranoside as acceptor substrates instead of MGn-PA, overview | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
beta-1,2-N-acetylglucosaminyltransferase II | - |
Homo sapiens |
GnTII | - |
Homo sapiens |
Mgat2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | beta-1,2-N-acetylglucosaminyltransferase II (GnTII) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans | Homo sapiens |