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Literature summary for 2.4.1.1 extracted from

  • Hsu, J.H.; Yang, C.C.; Su, J.C.; Lee, P.D.
    Purification and characterization of a cytosolic starch phosphorylase from etiolated rice seedlings (2004), Bot. Bull. Acad. Sin., 45, 187-196.
No PubMed abstract available
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Oryza sativa
DTT slight activation Oryza sativa
glutathione slight activation Oryza sativa
L-cysteine slight activation Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
ADP-glucose strong inhibition Oryza sativa
AMP slight inhibition Oryza sativa
Cyclodextrins
-
 Oryza sativa
D-fructose 6-phosphate strong inhibition Oryza sativa
D-mannose 1-phosphate strong inhibition Oryza sativa
IMP slight inhibition Oryza sativa
additional information no or poor inhibition by ribose 5-phosphate, glucose 6-phosphate, fructose-1,6-bisphosphate, ADP and UDP Oryza sativa
UDP-glucose strong inhibition Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
 amylopectin pH 5.5, 37°C, synthesis reaction Oryza sativa
0.0106
-
 soluble starch pH 5.5, 37°C, synthesis reaction Oryza sativa
0.0138
-
 glycogen pH 5.5, 37°C, synthesis reaction Oryza sativa
0.0261
-
 soluble starch pH 5.5, 37°C, synthesis reaction Oryza sativa
0.0418
-
 Dextrin pH 5.5, 37°C, synthesis reaction Oryza sativa
2.1
-
 alpha-D-glucose 1-phosphate pH 5.5, 37°C, synthesis reaction Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryza sativa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Na+, K+, Mg2+, Ca2+ Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1,4-alpha-D-glucosyl)n + phosphate Oryza sativa polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cv. Tainong 67, enzyme Pho1
-

Purification (Commentary)

Purification (Comment) Organism
from seedlings, 299fold to homogeneity by protamine sulfate and ammonium sulfate fractionation, anion exchange chromatography, and dextrin affinity chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated, low enzyme content Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Oryza sativa
1.8
-
 purified enzyme Oryza sativa

Storage Stability

Storage Stability Organism
purified enzyme in 50 mM imidazole, pH 7.0, 1 mM EDTA, 1 mM DTT, at 4°C, at least 2 weeks stable Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-glucosyl)n + phosphate polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme Oryza sativa (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
(1,4-alpha-D-glucosyl)n + phosphate polysaccharide substrates are soluble starch and Zulkowsky soluble starch, amylopectin, glycogen, and dextrin, amyylopectin is the best substrate Oryza sativa (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
amylopectin + phosphate
-
 Oryza sativa amylopectin + D-glucose-1-phosphate
-
 ?
dextrin + alpha-D-glucose 1-phosphate
-
 Oryza sativa dextrin + phosphate
-
 ?
glycogen + phosphate
-
 Oryza sativa alpha-D-glucan + alpha-D-glucose 1-phosphate
-
 r
maltodecaose + alpha-D-glucose 1-phosphate low activity Oryza sativa ? + phosphate
-
 r
maltoheptaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltooctaose + phosphate
-
 r
maltohexaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltoheptaose + phosphate
-
 r
maltononaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltodecaose + phosphate
-
 r
maltooctaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltononaose + phosphate
-
 r
maltopentaose + alpha-D-glucose 1-phosphate
-
 Oryza sativa maltohexaose + phosphate
-
 r
maltotetraose + alpha-D-glucose 1-phosphate
-
 Oryza sativa maltopentaose + phosphate
-
 r
additional information substrate specificity, no or nearly no activity with glucose, maltose, and maltotriose, overview Oryza sativa ?
-
 ?
soluble starch + phosphate
-
 Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-glucan phosphorylase
-
 Oryza sativa
PHO
-
 Oryza sativa
SP
-
 Oryza sativa
starch phosphorylase
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 synthesis reaction Oryza sativa
6
-
 assay at, phosphorolysis reaction Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 most stable at Oryza sativa