General Stability | Organism |
---|---|
phosphate protects against thermal inactivation | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by AMP | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
90000 | - |
2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
174000 | - |
high speed sedimentation equilibrium | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, polyethylenglycol, DEAE-cellulose, CM-Sephadex | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.7 | - |
maltoheptaose | Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
4°C, 3.5 M (NH4)2SO4 in solution of neutral pH-value, several months, no loss of activity | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(maltodextrin)n-1 + glucose 1-phosphate | glucosylation at 96% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | (maltodextrin)n + phosphate | - |
r | |
amylopectin + glucose 1-phosphate | glucosylation at 23% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
amylopectin + glucose 1-phosphate | waxy maize amylopectin | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
amylose + glucose 1-phosphate | glucosylation at 7% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylose + phosphate | - |
? | |
glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
glycogen + glucose 1-phosphate | glucosylation at 14.4% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
maltoheptaose + glucose 1-phosphate | - |
Klebsiella pneumoniae | maltooctaose + phosphate | - |
r | |
maltotetraose + glucose 1-phosphate | glucosylation at 22% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | maltopentaose + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | no activity with pullulan | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | no activity with dextran | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
dimer | 2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
phosphate protects against thermal inactivation | Klebsiella pneumoniae |
30 | - |
30 min stable at pH 5.9-10.5 | Klebsiella pneumoniae |
43 | - |
10 min stable | Klebsiella pneumoniae |
55 | - |
loss of approx. 90% activity after 10 min in 500 mM potassium phosphate pH 7.3 | Klebsiella pneumoniae |
63 | - |
inactivation within 10 min | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.3 | Klebsiella pneumoniae |
5.9 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.1 | 7.5 | approx. half-maximal activity at pH 5.1 and 7.5 | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.9 | 10.5 | 30 min stable at 30°C | Klebsiella pneumoniae |
11.5 | - |
and above, denaturation | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol per mol enzyme | Klebsiella pneumoniae |