Protein Variants | Comment | Organism |
---|---|---|
D453A | 0.06% of wild-type activity | Streptomyces coelicolor |
R171L | 0.2% of wild-type activity, reduced expression at room temperature, expression of soluble protein at 15°C | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
x * 86000, GST-enzyme fusion protein, SDS-PAGE | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9RKU9 | expression in Escherichia coli | - |
Streptomyces coelicolor A3(2) | Q9RKU9 | expression in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.6 | - |
mutant D453A, pH 7.9 | Streptomyces coelicolor |
44.2 | - |
mutant R171L, pH 7.9 | Streptomyces coelicolor |
25090 | - |
wild-type, pH 7.9 | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glyoxylate + H2O | - |
Streptomyces coelicolor | (S)-malate + CoA | - |
? | |
acetyl-CoA + glyoxylate + H2O | - |
Streptomyces coelicolor A3(2) | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 86000, GST-enzyme fusion protein, SDS-PAGE | Streptomyces coelicolor |